The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity

Cancer metabolic reprogramming enhances its malignant behaviors and drug resistance, which is regulated by POU domain transcription factors. This study explored the effect of POU domain class 2 transcription factor 1 (POU2F1) on metabolic reprogramming in colon cancer. The POU2F1 expression was anal...

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Autores principales: Lin, Jinguan, Xia, Longzheng, Oyang, Linda, Liang, Jiaxin, Tan, Shiming, Wu, Nayiyuan, Yi, Pin, Pan, Qing, Rao, Shan, Han, Yaqian, Tang, Yanyan, Su, Min, Luo, Xia, Yang, Yiqing, Chen, Xiaohui, Yang, Lixia, Zhou, Yujuan, Liao, Qianjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837540/
https://www.ncbi.nlm.nih.gov/pubmed/34997215
http://dx.doi.org/10.1038/s41388-021-02148-y
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author Lin, Jinguan
Xia, Longzheng
Oyang, Linda
Liang, Jiaxin
Tan, Shiming
Wu, Nayiyuan
Yi, Pin
Pan, Qing
Rao, Shan
Han, Yaqian
Tang, Yanyan
Su, Min
Luo, Xia
Yang, Yiqing
Chen, Xiaohui
Yang, Lixia
Zhou, Yujuan
Liao, Qianjin
author_facet Lin, Jinguan
Xia, Longzheng
Oyang, Linda
Liang, Jiaxin
Tan, Shiming
Wu, Nayiyuan
Yi, Pin
Pan, Qing
Rao, Shan
Han, Yaqian
Tang, Yanyan
Su, Min
Luo, Xia
Yang, Yiqing
Chen, Xiaohui
Yang, Lixia
Zhou, Yujuan
Liao, Qianjin
author_sort Lin, Jinguan
collection PubMed
description Cancer metabolic reprogramming enhances its malignant behaviors and drug resistance, which is regulated by POU domain transcription factors. This study explored the effect of POU domain class 2 transcription factor 1 (POU2F1) on metabolic reprogramming in colon cancer. The POU2F1 expression was analyzed in GEO dataset, TCGA cohorts and human colon cancer tissues by bioinformatics and immunohistochemistry. The effects of altered POU2F1 expression on proliferation, glucose metabolism and oxaliplatin sensitivity of colon cancer cells were tested. The impacts of POU2F1 on aldolase A (ALDOA) expression and malignant behaviors of colon cancer cells were examined. We found that up-regulated POU2F1 expression was associated with worse prognosis and oxaliplatin resistance in colon cancer. POU2F1 enhanced the proliferation, aerobic glycolysis and the pentose phosphate pathway (PPP) activity, but reduced oxidative stress and apoptosis in colon cancer cells, dependent on up-regulating ALDOA expression. Mechanistically, POU2F1 directly bound to the ALDOA promoter to enhance the ALDOA promoter activity in colon cancer cells. Moreover, activation of the POU2F1-ALDOA axis decreased the sensitivity to oxaliplatin in colon cancer cells. These data indicate that the POU2F1-ALDOA axis promotes the progression and oxaliplatin resistance by enhancing metabolic reprogramming in colon cancer. Our findings suggest that the POU2F1-ALDOA axis may be new therapeutic targets to overcome oxaliplatin resistance in colon cancer.
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spelling pubmed-88375402022-02-24 The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity Lin, Jinguan Xia, Longzheng Oyang, Linda Liang, Jiaxin Tan, Shiming Wu, Nayiyuan Yi, Pin Pan, Qing Rao, Shan Han, Yaqian Tang, Yanyan Su, Min Luo, Xia Yang, Yiqing Chen, Xiaohui Yang, Lixia Zhou, Yujuan Liao, Qianjin Oncogene Article Cancer metabolic reprogramming enhances its malignant behaviors and drug resistance, which is regulated by POU domain transcription factors. This study explored the effect of POU domain class 2 transcription factor 1 (POU2F1) on metabolic reprogramming in colon cancer. The POU2F1 expression was analyzed in GEO dataset, TCGA cohorts and human colon cancer tissues by bioinformatics and immunohistochemistry. The effects of altered POU2F1 expression on proliferation, glucose metabolism and oxaliplatin sensitivity of colon cancer cells were tested. The impacts of POU2F1 on aldolase A (ALDOA) expression and malignant behaviors of colon cancer cells were examined. We found that up-regulated POU2F1 expression was associated with worse prognosis and oxaliplatin resistance in colon cancer. POU2F1 enhanced the proliferation, aerobic glycolysis and the pentose phosphate pathway (PPP) activity, but reduced oxidative stress and apoptosis in colon cancer cells, dependent on up-regulating ALDOA expression. Mechanistically, POU2F1 directly bound to the ALDOA promoter to enhance the ALDOA promoter activity in colon cancer cells. Moreover, activation of the POU2F1-ALDOA axis decreased the sensitivity to oxaliplatin in colon cancer cells. These data indicate that the POU2F1-ALDOA axis promotes the progression and oxaliplatin resistance by enhancing metabolic reprogramming in colon cancer. Our findings suggest that the POU2F1-ALDOA axis may be new therapeutic targets to overcome oxaliplatin resistance in colon cancer. Nature Publishing Group UK 2022-01-08 2022 /pmc/articles/PMC8837540/ /pubmed/34997215 http://dx.doi.org/10.1038/s41388-021-02148-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lin, Jinguan
Xia, Longzheng
Oyang, Linda
Liang, Jiaxin
Tan, Shiming
Wu, Nayiyuan
Yi, Pin
Pan, Qing
Rao, Shan
Han, Yaqian
Tang, Yanyan
Su, Min
Luo, Xia
Yang, Yiqing
Chen, Xiaohui
Yang, Lixia
Zhou, Yujuan
Liao, Qianjin
The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity
title The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity
title_full The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity
title_fullStr The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity
title_full_unstemmed The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity
title_short The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity
title_sort pou2f1-aldoa axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837540/
https://www.ncbi.nlm.nih.gov/pubmed/34997215
http://dx.doi.org/10.1038/s41388-021-02148-y
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