Cargando…

Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits

Methyl jasmonate hormone can stimulate the production of several metabolites responsible for improving fruit quality and nutritional attributes related to human health. In this context, efforts to manipulate tomatoes, such as using hormonal treatment to increase metabolite levels essential to plant...

Descripción completa

Detalles Bibliográficos
Autores principales: Meza, Silvia Leticia Rivero, de Castro Tobaruela, Eric, Pascoal, Grazieli Benedetti, Magalhães, Hilton César Rodrigues, Massaretto, Isabel Louro, Purgatto, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838126/
https://www.ncbi.nlm.nih.gov/pubmed/35161348
http://dx.doi.org/10.3390/plants11030366
_version_ 1784650048854818816
author Meza, Silvia Leticia Rivero
de Castro Tobaruela, Eric
Pascoal, Grazieli Benedetti
Magalhães, Hilton César Rodrigues
Massaretto, Isabel Louro
Purgatto, Eduardo
author_facet Meza, Silvia Leticia Rivero
de Castro Tobaruela, Eric
Pascoal, Grazieli Benedetti
Magalhães, Hilton César Rodrigues
Massaretto, Isabel Louro
Purgatto, Eduardo
author_sort Meza, Silvia Leticia Rivero
collection PubMed
description Methyl jasmonate hormone can stimulate the production of several metabolites responsible for improving fruit quality and nutritional attributes related to human health. In this context, efforts to manipulate tomatoes, such as using hormonal treatment to increase metabolite levels essential to plant growth and human nutrition, have received considerable attention. The aim of this study was to show the impact of metabolic profile on fruit quality and nutritional properties under exogenous methyl jasmonate during fruit ripening. The treatments were performed using 100 ppm of methyl jasmonate and 100 ppm of gaseous ethylene over 24 h. Ethylene emission, fruit surface color and metabolomics analysis were measured at 4, 10, and 21 days after harvest, considering the untreated fruits as control group. Methyl jasmonate induced the production of amino acids—mainly glutamine, glutamic acid and γ-aminobutyric acid (at least 14-fold higher)—and fatty acids—mainly oleic, linoleic, and linolenic acids (at least three-fold higher than untreated fruits); while exogenous ethylene predominantly affected sugar metabolism, increasing the levels of fructose, mannose and glucose to at least two-fold that levels in the untreated fruits. Additionally, methyl jasmonate significantly affected secondary metabolites, inducing by at least 80% the accumulation of α-tocopherol and β-sitosterol in fully ripe fruits. Our results suggest that the postharvest application of the hormone methyl jasmonate can contribute to the sensory characteristics and increase the nutritional value of the fruits since important changes related to the tomato metabolome were associated with compounds responsible for the fruit quality and health benefits.
format Online
Article
Text
id pubmed-8838126
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88381262022-02-13 Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits Meza, Silvia Leticia Rivero de Castro Tobaruela, Eric Pascoal, Grazieli Benedetti Magalhães, Hilton César Rodrigues Massaretto, Isabel Louro Purgatto, Eduardo Plants (Basel) Article Methyl jasmonate hormone can stimulate the production of several metabolites responsible for improving fruit quality and nutritional attributes related to human health. In this context, efforts to manipulate tomatoes, such as using hormonal treatment to increase metabolite levels essential to plant growth and human nutrition, have received considerable attention. The aim of this study was to show the impact of metabolic profile on fruit quality and nutritional properties under exogenous methyl jasmonate during fruit ripening. The treatments were performed using 100 ppm of methyl jasmonate and 100 ppm of gaseous ethylene over 24 h. Ethylene emission, fruit surface color and metabolomics analysis were measured at 4, 10, and 21 days after harvest, considering the untreated fruits as control group. Methyl jasmonate induced the production of amino acids—mainly glutamine, glutamic acid and γ-aminobutyric acid (at least 14-fold higher)—and fatty acids—mainly oleic, linoleic, and linolenic acids (at least three-fold higher than untreated fruits); while exogenous ethylene predominantly affected sugar metabolism, increasing the levels of fructose, mannose and glucose to at least two-fold that levels in the untreated fruits. Additionally, methyl jasmonate significantly affected secondary metabolites, inducing by at least 80% the accumulation of α-tocopherol and β-sitosterol in fully ripe fruits. Our results suggest that the postharvest application of the hormone methyl jasmonate can contribute to the sensory characteristics and increase the nutritional value of the fruits since important changes related to the tomato metabolome were associated with compounds responsible for the fruit quality and health benefits. MDPI 2022-01-28 /pmc/articles/PMC8838126/ /pubmed/35161348 http://dx.doi.org/10.3390/plants11030366 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meza, Silvia Leticia Rivero
de Castro Tobaruela, Eric
Pascoal, Grazieli Benedetti
Magalhães, Hilton César Rodrigues
Massaretto, Isabel Louro
Purgatto, Eduardo
Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits
title Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits
title_full Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits
title_fullStr Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits
title_full_unstemmed Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits
title_short Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits
title_sort induction of metabolic changes in amino acid, fatty acid, tocopherol, and phytosterol profiles by exogenous methyl jasmonate application in tomato fruits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838126/
https://www.ncbi.nlm.nih.gov/pubmed/35161348
http://dx.doi.org/10.3390/plants11030366
work_keys_str_mv AT mezasilvialeticiarivero inductionofmetabolicchangesinaminoacidfattyacidtocopherolandphytosterolprofilesbyexogenousmethyljasmonateapplicationintomatofruits
AT decastrotobaruelaeric inductionofmetabolicchangesinaminoacidfattyacidtocopherolandphytosterolprofilesbyexogenousmethyljasmonateapplicationintomatofruits
AT pascoalgrazielibenedetti inductionofmetabolicchangesinaminoacidfattyacidtocopherolandphytosterolprofilesbyexogenousmethyljasmonateapplicationintomatofruits
AT magalhaeshiltoncesarrodrigues inductionofmetabolicchangesinaminoacidfattyacidtocopherolandphytosterolprofilesbyexogenousmethyljasmonateapplicationintomatofruits
AT massarettoisabellouro inductionofmetabolicchangesinaminoacidfattyacidtocopherolandphytosterolprofilesbyexogenousmethyljasmonateapplicationintomatofruits
AT purgattoeduardo inductionofmetabolicchangesinaminoacidfattyacidtocopherolandphytosterolprofilesbyexogenousmethyljasmonateapplicationintomatofruits