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Putative Genes of Pathogenesis-Related Proteins and Coronatine-Insensitive Protein 1 in Ribes spp.

In response to pathogen attacks, plants activate a complex of defense mechanisms including an accumulation of the endogenous signaling compounds salicylic acid and jasmonic acid. The activity of pathogenesis-related genes (PRs) and coronatine-insensitive 1 (COI1) in defense-response pathways are est...

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Autores principales: Juškytė, Ana Dovilė, Mažeikienė, Ingrida, Stanys, Vidmantas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838371/
https://www.ncbi.nlm.nih.gov/pubmed/35161336
http://dx.doi.org/10.3390/plants11030355
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author Juškytė, Ana Dovilė
Mažeikienė, Ingrida
Stanys, Vidmantas
author_facet Juškytė, Ana Dovilė
Mažeikienė, Ingrida
Stanys, Vidmantas
author_sort Juškytė, Ana Dovilė
collection PubMed
description In response to pathogen attacks, plants activate a complex of defense mechanisms including an accumulation of the endogenous signaling compounds salicylic acid and jasmonic acid. The activity of pathogenesis-related genes (PRs) and coronatine-insensitive 1 (COI1) in defense-response pathways are established in plants. The aim of this study was to identify homologs of the PRs and COI1 in blackcurrants. Primers with degenerate nucleotides were designed based on the most conservative parts of PR1 and COI1 genes from other plants and applied for amplification of specific fragments of PRs and COI1 in Ribes spp. Seven heterogeneous sequences of PR with a diversity of 66.0–98.3% at nucleic acid level were found. The phylogenetic analysis revealed the dependence of R. nigrum PR homologs on the PR1 and PR6 families. Four heterogeneous sequences of R. nigrum COI1 with an identity of 95.9–98.8% at nucleic acid level were isolated. Specific primers for newly detected genes’ homologs were designed in this study and could be useful for evaluating the defense response to pathogen attacks in blackcurrants.
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spelling pubmed-88383712022-02-13 Putative Genes of Pathogenesis-Related Proteins and Coronatine-Insensitive Protein 1 in Ribes spp. Juškytė, Ana Dovilė Mažeikienė, Ingrida Stanys, Vidmantas Plants (Basel) Article In response to pathogen attacks, plants activate a complex of defense mechanisms including an accumulation of the endogenous signaling compounds salicylic acid and jasmonic acid. The activity of pathogenesis-related genes (PRs) and coronatine-insensitive 1 (COI1) in defense-response pathways are established in plants. The aim of this study was to identify homologs of the PRs and COI1 in blackcurrants. Primers with degenerate nucleotides were designed based on the most conservative parts of PR1 and COI1 genes from other plants and applied for amplification of specific fragments of PRs and COI1 in Ribes spp. Seven heterogeneous sequences of PR with a diversity of 66.0–98.3% at nucleic acid level were found. The phylogenetic analysis revealed the dependence of R. nigrum PR homologs on the PR1 and PR6 families. Four heterogeneous sequences of R. nigrum COI1 with an identity of 95.9–98.8% at nucleic acid level were isolated. Specific primers for newly detected genes’ homologs were designed in this study and could be useful for evaluating the defense response to pathogen attacks in blackcurrants. MDPI 2022-01-28 /pmc/articles/PMC8838371/ /pubmed/35161336 http://dx.doi.org/10.3390/plants11030355 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Juškytė, Ana Dovilė
Mažeikienė, Ingrida
Stanys, Vidmantas
Putative Genes of Pathogenesis-Related Proteins and Coronatine-Insensitive Protein 1 in Ribes spp.
title Putative Genes of Pathogenesis-Related Proteins and Coronatine-Insensitive Protein 1 in Ribes spp.
title_full Putative Genes of Pathogenesis-Related Proteins and Coronatine-Insensitive Protein 1 in Ribes spp.
title_fullStr Putative Genes of Pathogenesis-Related Proteins and Coronatine-Insensitive Protein 1 in Ribes spp.
title_full_unstemmed Putative Genes of Pathogenesis-Related Proteins and Coronatine-Insensitive Protein 1 in Ribes spp.
title_short Putative Genes of Pathogenesis-Related Proteins and Coronatine-Insensitive Protein 1 in Ribes spp.
title_sort putative genes of pathogenesis-related proteins and coronatine-insensitive protein 1 in ribes spp.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838371/
https://www.ncbi.nlm.nih.gov/pubmed/35161336
http://dx.doi.org/10.3390/plants11030355
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