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Evaluation of the Effects of Cold Plasma on Cell Membrane Lipids and Oxidative Injury of Salmonella typhimurium

Salmonella typhimurium (S. typhimurium) is a major causative agent of foodborne illness worldwide. Cold plasma (CP) was used to inactivate S. typhimurium and to investigate the effect of CP on cell membrane lipids and oxidative injury of cells. Results indicated that the inactivation effect of CP on...

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Autores principales: Lv, Xiaoye, Cheng, Jun-Hu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838372/
https://www.ncbi.nlm.nih.gov/pubmed/35163904
http://dx.doi.org/10.3390/molecules27030640
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author Lv, Xiaoye
Cheng, Jun-Hu
author_facet Lv, Xiaoye
Cheng, Jun-Hu
author_sort Lv, Xiaoye
collection PubMed
description Salmonella typhimurium (S. typhimurium) is a major causative agent of foodborne illness worldwide. Cold plasma (CP) was used to inactivate S. typhimurium and to investigate the effect of CP on cell membrane lipids and oxidative injury of cells. Results indicated that the inactivation effect of CP on S. typhimurium was positively correlated with the treatment time and voltage. S. typhimurium was undetectable (total number of surviving colonies <2 log CFU/mL) after 5 min treatment with the voltage of 50 V. CP treatment caused damage to the cell membrane of S. typhimurium and the leakage of cell contents, and the relative content of unsaturated fatty acids in cell membrane decreased. Cell membrane lipids were oxidized; the malondialdehyde content increased from 0.219 nmol/mL to 0.658 nmol/mL; the catalase activity of S. typhimurium solution increased from 751 U/mL to 2542 U/mL; and the total superoxide dismutase activity increased from 3.076 U/mL to 4.54 U/mL, which confirmed the oxidative damage in S. typhimurium cell membrane caused by CP treatment. It was demonstrated that the potential application of plasma-mediated reactive oxygen species is suitable for destroying the structures of the cell membrane and ensuring the microbial safety of fresh food samples.
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spelling pubmed-88383722022-02-13 Evaluation of the Effects of Cold Plasma on Cell Membrane Lipids and Oxidative Injury of Salmonella typhimurium Lv, Xiaoye Cheng, Jun-Hu Molecules Article Salmonella typhimurium (S. typhimurium) is a major causative agent of foodborne illness worldwide. Cold plasma (CP) was used to inactivate S. typhimurium and to investigate the effect of CP on cell membrane lipids and oxidative injury of cells. Results indicated that the inactivation effect of CP on S. typhimurium was positively correlated with the treatment time and voltage. S. typhimurium was undetectable (total number of surviving colonies <2 log CFU/mL) after 5 min treatment with the voltage of 50 V. CP treatment caused damage to the cell membrane of S. typhimurium and the leakage of cell contents, and the relative content of unsaturated fatty acids in cell membrane decreased. Cell membrane lipids were oxidized; the malondialdehyde content increased from 0.219 nmol/mL to 0.658 nmol/mL; the catalase activity of S. typhimurium solution increased from 751 U/mL to 2542 U/mL; and the total superoxide dismutase activity increased from 3.076 U/mL to 4.54 U/mL, which confirmed the oxidative damage in S. typhimurium cell membrane caused by CP treatment. It was demonstrated that the potential application of plasma-mediated reactive oxygen species is suitable for destroying the structures of the cell membrane and ensuring the microbial safety of fresh food samples. MDPI 2022-01-19 /pmc/articles/PMC8838372/ /pubmed/35163904 http://dx.doi.org/10.3390/molecules27030640 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lv, Xiaoye
Cheng, Jun-Hu
Evaluation of the Effects of Cold Plasma on Cell Membrane Lipids and Oxidative Injury of Salmonella typhimurium
title Evaluation of the Effects of Cold Plasma on Cell Membrane Lipids and Oxidative Injury of Salmonella typhimurium
title_full Evaluation of the Effects of Cold Plasma on Cell Membrane Lipids and Oxidative Injury of Salmonella typhimurium
title_fullStr Evaluation of the Effects of Cold Plasma on Cell Membrane Lipids and Oxidative Injury of Salmonella typhimurium
title_full_unstemmed Evaluation of the Effects of Cold Plasma on Cell Membrane Lipids and Oxidative Injury of Salmonella typhimurium
title_short Evaluation of the Effects of Cold Plasma on Cell Membrane Lipids and Oxidative Injury of Salmonella typhimurium
title_sort evaluation of the effects of cold plasma on cell membrane lipids and oxidative injury of salmonella typhimurium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838372/
https://www.ncbi.nlm.nih.gov/pubmed/35163904
http://dx.doi.org/10.3390/molecules27030640
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