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Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming

Stress priming is an important strategy for enhancing plant defense capacity to deal with environmental challenges and involves reprogrammed transcriptional responses. Although ultraviolet (UV) light exposure is a widely adopted approach to elicit stress memory and tolerance in plants, the molecular...

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Autores principales: Martins, Andressa Cunha Quintana, Mota, Ana Paula Zotta, Carvalho, Paula Andrea Sampaio Vasconcelos, Passos, Mario Alfredo Saraiva, Gimenes, Marcos Aparecido, Guimaraes, Patricia Messenberg, Brasileiro, Ana Cristina Miranda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838480/
https://www.ncbi.nlm.nih.gov/pubmed/35161389
http://dx.doi.org/10.3390/plants11030408
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author Martins, Andressa Cunha Quintana
Mota, Ana Paula Zotta
Carvalho, Paula Andrea Sampaio Vasconcelos
Passos, Mario Alfredo Saraiva
Gimenes, Marcos Aparecido
Guimaraes, Patricia Messenberg
Brasileiro, Ana Cristina Miranda
author_facet Martins, Andressa Cunha Quintana
Mota, Ana Paula Zotta
Carvalho, Paula Andrea Sampaio Vasconcelos
Passos, Mario Alfredo Saraiva
Gimenes, Marcos Aparecido
Guimaraes, Patricia Messenberg
Brasileiro, Ana Cristina Miranda
author_sort Martins, Andressa Cunha Quintana
collection PubMed
description Stress priming is an important strategy for enhancing plant defense capacity to deal with environmental challenges and involves reprogrammed transcriptional responses. Although ultraviolet (UV) light exposure is a widely adopted approach to elicit stress memory and tolerance in plants, the molecular mechanisms underlying UV-mediated plant priming tolerance are not fully understood. Here, we investigated the changes in the global transcriptome profile of wild Arachis stenosperma leaves in response to UV-C exposure. A total of 5751 differentially expressed genes (DEGs) were identified, with the majority associated with cell signaling, protein dynamics, hormonal and transcriptional regulation, and secondary metabolic pathways. The expression profiles of DEGs known as indicators of priming state, such as transcription factors, transcriptional regulators and protein kinases, were further characterized. A meta-analysis, followed by qRT-PCR validation, identified 18 metaDEGs as being commonly regulated in response to UV and other primary stresses. These genes are involved in secondary metabolism, basal immunity, cell wall structure and integrity, and may constitute important players in the general defense processes and establishment of a priming state in A. stenosperma. Our findings contribute to a better understanding of transcriptional dynamics involved in wild Arachis adaptation to stressful conditions of their natural habitats.
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spelling pubmed-88384802022-02-13 Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming Martins, Andressa Cunha Quintana Mota, Ana Paula Zotta Carvalho, Paula Andrea Sampaio Vasconcelos Passos, Mario Alfredo Saraiva Gimenes, Marcos Aparecido Guimaraes, Patricia Messenberg Brasileiro, Ana Cristina Miranda Plants (Basel) Article Stress priming is an important strategy for enhancing plant defense capacity to deal with environmental challenges and involves reprogrammed transcriptional responses. Although ultraviolet (UV) light exposure is a widely adopted approach to elicit stress memory and tolerance in plants, the molecular mechanisms underlying UV-mediated plant priming tolerance are not fully understood. Here, we investigated the changes in the global transcriptome profile of wild Arachis stenosperma leaves in response to UV-C exposure. A total of 5751 differentially expressed genes (DEGs) were identified, with the majority associated with cell signaling, protein dynamics, hormonal and transcriptional regulation, and secondary metabolic pathways. The expression profiles of DEGs known as indicators of priming state, such as transcription factors, transcriptional regulators and protein kinases, were further characterized. A meta-analysis, followed by qRT-PCR validation, identified 18 metaDEGs as being commonly regulated in response to UV and other primary stresses. These genes are involved in secondary metabolism, basal immunity, cell wall structure and integrity, and may constitute important players in the general defense processes and establishment of a priming state in A. stenosperma. Our findings contribute to a better understanding of transcriptional dynamics involved in wild Arachis adaptation to stressful conditions of their natural habitats. MDPI 2022-02-02 /pmc/articles/PMC8838480/ /pubmed/35161389 http://dx.doi.org/10.3390/plants11030408 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martins, Andressa Cunha Quintana
Mota, Ana Paula Zotta
Carvalho, Paula Andrea Sampaio Vasconcelos
Passos, Mario Alfredo Saraiva
Gimenes, Marcos Aparecido
Guimaraes, Patricia Messenberg
Brasileiro, Ana Cristina Miranda
Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming
title Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming
title_full Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming
title_fullStr Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming
title_full_unstemmed Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming
title_short Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming
title_sort transcriptome responses of wild arachis to uv-c exposure reveal genes involved in general plant defense and priming
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838480/
https://www.ncbi.nlm.nih.gov/pubmed/35161389
http://dx.doi.org/10.3390/plants11030408
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