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De Novo Transcriptome of Mammillaria bombycina (Cactaceae) under In Vitro Conditions and Identification of Glyoxalase Genes
Mammillaria bombycina is a cactus distributed in the central region of Mexico. Cactaceae have the particularity of surviving drought and high temperatures, which is why in vitro propagation studies have been carried out successfully to preserve this species and use it as a study model in cacti. In t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838482/ https://www.ncbi.nlm.nih.gov/pubmed/35161380 http://dx.doi.org/10.3390/plants11030399 |
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author | Enríquez-González, Carolina Garcidueñas-Piña, Cristina Castellanos-Hernández, Osvaldo Adrián Enríquez-Aranda, Sergio Loera-Muro, Abraham Ocampo, Gilberto Pérez-Molphe Balch, Eugenio Morales-Domínguez, José Francisco |
author_facet | Enríquez-González, Carolina Garcidueñas-Piña, Cristina Castellanos-Hernández, Osvaldo Adrián Enríquez-Aranda, Sergio Loera-Muro, Abraham Ocampo, Gilberto Pérez-Molphe Balch, Eugenio Morales-Domínguez, José Francisco |
author_sort | Enríquez-González, Carolina |
collection | PubMed |
description | Mammillaria bombycina is a cactus distributed in the central region of Mexico. Cactaceae have the particularity of surviving drought and high temperatures, which is why in vitro propagation studies have been carried out successfully to preserve this species and use it as a study model in cacti. In this contribution, a de novo transcriptome of M. bombycina was produced under in vitro conditions for the identification and expression of genes related to abiotic stress. Samples were sequenced using an Illumina platform, averaging 24 million clean readings. From assembly and annotation, 84,975 transcripts were generated, 55% of which were unigenes. Among these, the presence of 13 isoforms of genes belonging to glyoxalase I, II and III were identified. An analysis of the qRT-PCR expression of these genes was performed under in vitro and ex vitro conditions and dehydration at 6 and 24 h. The highest expression was observed under greenhouse conditions and dehydration at 24 h, according to the control. The de novo assembly of the M. bombycina transcriptome remains a study model for future work in cacti. |
format | Online Article Text |
id | pubmed-8838482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88384822022-02-13 De Novo Transcriptome of Mammillaria bombycina (Cactaceae) under In Vitro Conditions and Identification of Glyoxalase Genes Enríquez-González, Carolina Garcidueñas-Piña, Cristina Castellanos-Hernández, Osvaldo Adrián Enríquez-Aranda, Sergio Loera-Muro, Abraham Ocampo, Gilberto Pérez-Molphe Balch, Eugenio Morales-Domínguez, José Francisco Plants (Basel) Article Mammillaria bombycina is a cactus distributed in the central region of Mexico. Cactaceae have the particularity of surviving drought and high temperatures, which is why in vitro propagation studies have been carried out successfully to preserve this species and use it as a study model in cacti. In this contribution, a de novo transcriptome of M. bombycina was produced under in vitro conditions for the identification and expression of genes related to abiotic stress. Samples were sequenced using an Illumina platform, averaging 24 million clean readings. From assembly and annotation, 84,975 transcripts were generated, 55% of which were unigenes. Among these, the presence of 13 isoforms of genes belonging to glyoxalase I, II and III were identified. An analysis of the qRT-PCR expression of these genes was performed under in vitro and ex vitro conditions and dehydration at 6 and 24 h. The highest expression was observed under greenhouse conditions and dehydration at 24 h, according to the control. The de novo assembly of the M. bombycina transcriptome remains a study model for future work in cacti. MDPI 2022-01-31 /pmc/articles/PMC8838482/ /pubmed/35161380 http://dx.doi.org/10.3390/plants11030399 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Enríquez-González, Carolina Garcidueñas-Piña, Cristina Castellanos-Hernández, Osvaldo Adrián Enríquez-Aranda, Sergio Loera-Muro, Abraham Ocampo, Gilberto Pérez-Molphe Balch, Eugenio Morales-Domínguez, José Francisco De Novo Transcriptome of Mammillaria bombycina (Cactaceae) under In Vitro Conditions and Identification of Glyoxalase Genes |
title | De Novo Transcriptome of Mammillaria bombycina (Cactaceae) under In Vitro Conditions and Identification of Glyoxalase Genes |
title_full | De Novo Transcriptome of Mammillaria bombycina (Cactaceae) under In Vitro Conditions and Identification of Glyoxalase Genes |
title_fullStr | De Novo Transcriptome of Mammillaria bombycina (Cactaceae) under In Vitro Conditions and Identification of Glyoxalase Genes |
title_full_unstemmed | De Novo Transcriptome of Mammillaria bombycina (Cactaceae) under In Vitro Conditions and Identification of Glyoxalase Genes |
title_short | De Novo Transcriptome of Mammillaria bombycina (Cactaceae) under In Vitro Conditions and Identification of Glyoxalase Genes |
title_sort | de novo transcriptome of mammillaria bombycina (cactaceae) under in vitro conditions and identification of glyoxalase genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838482/ https://www.ncbi.nlm.nih.gov/pubmed/35161380 http://dx.doi.org/10.3390/plants11030399 |
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