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Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults

From tilapia (Oreochromis niloticus) farming, the by-products have been identified as a source of collagen that could be used for the development of dermocosmetics or pharmaceutical products. However, the characteristics of collagen related to a specific strain or culture must be well defined prior...

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Autores principales: Reátegui-Pinedo, Nataly, Salirrosas, David, Sánchez-Tuesta, Linda, Quiñones, Claudio, Jáuregui-Rosas, Segundo R., Barraza, Gabriela, Cabrera, Angelita, Ayala-Jara, Carmen, Martinez, Renata Miliani, Baby, André Rolim, Prieto, Zulita Adriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838504/
https://www.ncbi.nlm.nih.gov/pubmed/35164387
http://dx.doi.org/10.3390/molecules27031123
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author Reátegui-Pinedo, Nataly
Salirrosas, David
Sánchez-Tuesta, Linda
Quiñones, Claudio
Jáuregui-Rosas, Segundo R.
Barraza, Gabriela
Cabrera, Angelita
Ayala-Jara, Carmen
Martinez, Renata Miliani
Baby, André Rolim
Prieto, Zulita Adriana
author_facet Reátegui-Pinedo, Nataly
Salirrosas, David
Sánchez-Tuesta, Linda
Quiñones, Claudio
Jáuregui-Rosas, Segundo R.
Barraza, Gabriela
Cabrera, Angelita
Ayala-Jara, Carmen
Martinez, Renata Miliani
Baby, André Rolim
Prieto, Zulita Adriana
author_sort Reátegui-Pinedo, Nataly
collection PubMed
description From tilapia (Oreochromis niloticus) farming, the by-products have been identified as a source of collagen that could be used for the development of dermocosmetics or pharmaceutical products. However, the characteristics of collagen related to a specific strain or culture must be well defined prior to its application. Collagen was extracted from the skin of three strains of tilapia: red YY males (YY: two Y-type sex chromosomes), XX gray females, and the F1: offspring of crossing red YY males with XX gray females; at different ages in the adult phase, using acetic acid and pepsin enzyme. The characteristics of acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were shown by SDS-PAGE band profiles to be similar to bovine collagen type I (SIGMA), the PSC of gray tilapia being more fragile to temperature changes, consistent with the results of fractional viscosity. The characteristics of the F1 progeny were prioritized for being a commercially productive and sustainable source for the extraction of collagen, and the ASC form, being the one with the greatest stability and advantage over PSC, of importance to our investigations, leads to a controlled digestion as in the case of peptide induction, and also in the development of natural products in the pharmaceutical and/or dermocosmetic industry. Evaluations of the triple helix structure by FT-IR, X-ray diffraction and UV–visible spectroscopy give similar results between the strains: red, gray, and F1, and between ages in the adult form F1 (15, 24, and 36 months of age). Consequently, the skin of tilapia in adult form is recommended sustainably for up to 24 months of age where the collagen is obtained with the use of acetic acid without enzymatic treatment.
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spelling pubmed-88385042022-02-13 Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults Reátegui-Pinedo, Nataly Salirrosas, David Sánchez-Tuesta, Linda Quiñones, Claudio Jáuregui-Rosas, Segundo R. Barraza, Gabriela Cabrera, Angelita Ayala-Jara, Carmen Martinez, Renata Miliani Baby, André Rolim Prieto, Zulita Adriana Molecules Article From tilapia (Oreochromis niloticus) farming, the by-products have been identified as a source of collagen that could be used for the development of dermocosmetics or pharmaceutical products. However, the characteristics of collagen related to a specific strain or culture must be well defined prior to its application. Collagen was extracted from the skin of three strains of tilapia: red YY males (YY: two Y-type sex chromosomes), XX gray females, and the F1: offspring of crossing red YY males with XX gray females; at different ages in the adult phase, using acetic acid and pepsin enzyme. The characteristics of acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were shown by SDS-PAGE band profiles to be similar to bovine collagen type I (SIGMA), the PSC of gray tilapia being more fragile to temperature changes, consistent with the results of fractional viscosity. The characteristics of the F1 progeny were prioritized for being a commercially productive and sustainable source for the extraction of collagen, and the ASC form, being the one with the greatest stability and advantage over PSC, of importance to our investigations, leads to a controlled digestion as in the case of peptide induction, and also in the development of natural products in the pharmaceutical and/or dermocosmetic industry. Evaluations of the triple helix structure by FT-IR, X-ray diffraction and UV–visible spectroscopy give similar results between the strains: red, gray, and F1, and between ages in the adult form F1 (15, 24, and 36 months of age). Consequently, the skin of tilapia in adult form is recommended sustainably for up to 24 months of age where the collagen is obtained with the use of acetic acid without enzymatic treatment. MDPI 2022-02-08 /pmc/articles/PMC8838504/ /pubmed/35164387 http://dx.doi.org/10.3390/molecules27031123 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reátegui-Pinedo, Nataly
Salirrosas, David
Sánchez-Tuesta, Linda
Quiñones, Claudio
Jáuregui-Rosas, Segundo R.
Barraza, Gabriela
Cabrera, Angelita
Ayala-Jara, Carmen
Martinez, Renata Miliani
Baby, André Rolim
Prieto, Zulita Adriana
Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults
title Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults
title_full Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults
title_fullStr Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults
title_full_unstemmed Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults
title_short Characterization of Collagen from Three Genetic Lines (Gray, Red and F1) of Oreochromis niloticus (Tilapia) Skin in Young and Old Adults
title_sort characterization of collagen from three genetic lines (gray, red and f1) of oreochromis niloticus (tilapia) skin in young and old adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838504/
https://www.ncbi.nlm.nih.gov/pubmed/35164387
http://dx.doi.org/10.3390/molecules27031123
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