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Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue

Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, Brassica napus L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a...

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Autores principales: Luo, Jing, Huang, Sha, Wang, Min, Zhang, Ruimao, Zhao, Degang, Yang, Yuanyu, Wang, Fang, Wang, Zhuanzhuan, Tang, Rong, Wang, Lulu, Xiao, Huagui, Yang, Bin, Li, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838640/
https://www.ncbi.nlm.nih.gov/pubmed/35161394
http://dx.doi.org/10.3390/plants11030413
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author Luo, Jing
Huang, Sha
Wang, Min
Zhang, Ruimao
Zhao, Degang
Yang, Yuanyu
Wang, Fang
Wang, Zhuanzhuan
Tang, Rong
Wang, Lulu
Xiao, Huagui
Yang, Bin
Li, Chao
author_facet Luo, Jing
Huang, Sha
Wang, Min
Zhang, Ruimao
Zhao, Degang
Yang, Yuanyu
Wang, Fang
Wang, Zhuanzhuan
Tang, Rong
Wang, Lulu
Xiao, Huagui
Yang, Bin
Li, Chao
author_sort Luo, Jing
collection PubMed
description Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, Brassica napus L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a high yield, high oil content, and is suitable for mechanized production. To fully comprehend the dwarfing mechanism of DW871 and provide a theoretical foundation for future applications of the variety, we used transcriptome and proteome sequencing to identify genes and proteins associated with the dwarfing phenotype, using homologous high-stalk material HW871 as a control. By RNA-seq and iTRAQ, we discovered 8665 DEGs and 50 DAPs. Comprehensive transcription and translation level analysis revealed 25 correlations, 23 of which have the same expression trend, involving monolignin synthesis, pectin-lignin assembly, lignification, glucose modification, cell wall composition and architecture, cell morphology, vascular bundle development, and stalk tissue composition and architecture. As a result of these results, we can formulate a hypothesis about the DW871 dwarfing phenotype: plant hormone signal transduction, such as IAA and BRs, is linked to the formation of dwarf phenotypes, and metabolic pathways related to lignin synthesis, such as phenylpropane biosynthesis, also play a role. Our works will contribute to a better understanding of the genes and proteins involved in the rapeseed dwarf phenotype, and we will propose new insights into the dwarfing mechanism of Brassica napus L.
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spelling pubmed-88386402022-02-13 Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue Luo, Jing Huang, Sha Wang, Min Zhang, Ruimao Zhao, Degang Yang, Yuanyu Wang, Fang Wang, Zhuanzhuan Tang, Rong Wang, Lulu Xiao, Huagui Yang, Bin Li, Chao Plants (Basel) Article Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, Brassica napus L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a high yield, high oil content, and is suitable for mechanized production. To fully comprehend the dwarfing mechanism of DW871 and provide a theoretical foundation for future applications of the variety, we used transcriptome and proteome sequencing to identify genes and proteins associated with the dwarfing phenotype, using homologous high-stalk material HW871 as a control. By RNA-seq and iTRAQ, we discovered 8665 DEGs and 50 DAPs. Comprehensive transcription and translation level analysis revealed 25 correlations, 23 of which have the same expression trend, involving monolignin synthesis, pectin-lignin assembly, lignification, glucose modification, cell wall composition and architecture, cell morphology, vascular bundle development, and stalk tissue composition and architecture. As a result of these results, we can formulate a hypothesis about the DW871 dwarfing phenotype: plant hormone signal transduction, such as IAA and BRs, is linked to the formation of dwarf phenotypes, and metabolic pathways related to lignin synthesis, such as phenylpropane biosynthesis, also play a role. Our works will contribute to a better understanding of the genes and proteins involved in the rapeseed dwarf phenotype, and we will propose new insights into the dwarfing mechanism of Brassica napus L. MDPI 2022-02-02 /pmc/articles/PMC8838640/ /pubmed/35161394 http://dx.doi.org/10.3390/plants11030413 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luo, Jing
Huang, Sha
Wang, Min
Zhang, Ruimao
Zhao, Degang
Yang, Yuanyu
Wang, Fang
Wang, Zhuanzhuan
Tang, Rong
Wang, Lulu
Xiao, Huagui
Yang, Bin
Li, Chao
Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue
title Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue
title_full Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue
title_fullStr Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue
title_full_unstemmed Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue
title_short Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue
title_sort characterization of the transcriptome and proteome of brassica napus reveals the close relation between dw871 dwarfing phenotype and stalk tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838640/
https://www.ncbi.nlm.nih.gov/pubmed/35161394
http://dx.doi.org/10.3390/plants11030413
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