Cargando…
Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue
Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, Brassica napus L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838640/ https://www.ncbi.nlm.nih.gov/pubmed/35161394 http://dx.doi.org/10.3390/plants11030413 |
_version_ | 1784650176276725760 |
---|---|
author | Luo, Jing Huang, Sha Wang, Min Zhang, Ruimao Zhao, Degang Yang, Yuanyu Wang, Fang Wang, Zhuanzhuan Tang, Rong Wang, Lulu Xiao, Huagui Yang, Bin Li, Chao |
author_facet | Luo, Jing Huang, Sha Wang, Min Zhang, Ruimao Zhao, Degang Yang, Yuanyu Wang, Fang Wang, Zhuanzhuan Tang, Rong Wang, Lulu Xiao, Huagui Yang, Bin Li, Chao |
author_sort | Luo, Jing |
collection | PubMed |
description | Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, Brassica napus L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a high yield, high oil content, and is suitable for mechanized production. To fully comprehend the dwarfing mechanism of DW871 and provide a theoretical foundation for future applications of the variety, we used transcriptome and proteome sequencing to identify genes and proteins associated with the dwarfing phenotype, using homologous high-stalk material HW871 as a control. By RNA-seq and iTRAQ, we discovered 8665 DEGs and 50 DAPs. Comprehensive transcription and translation level analysis revealed 25 correlations, 23 of which have the same expression trend, involving monolignin synthesis, pectin-lignin assembly, lignification, glucose modification, cell wall composition and architecture, cell morphology, vascular bundle development, and stalk tissue composition and architecture. As a result of these results, we can formulate a hypothesis about the DW871 dwarfing phenotype: plant hormone signal transduction, such as IAA and BRs, is linked to the formation of dwarf phenotypes, and metabolic pathways related to lignin synthesis, such as phenylpropane biosynthesis, also play a role. Our works will contribute to a better understanding of the genes and proteins involved in the rapeseed dwarf phenotype, and we will propose new insights into the dwarfing mechanism of Brassica napus L. |
format | Online Article Text |
id | pubmed-8838640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88386402022-02-13 Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue Luo, Jing Huang, Sha Wang, Min Zhang, Ruimao Zhao, Degang Yang, Yuanyu Wang, Fang Wang, Zhuanzhuan Tang, Rong Wang, Lulu Xiao, Huagui Yang, Bin Li, Chao Plants (Basel) Article Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, Brassica napus L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a high yield, high oil content, and is suitable for mechanized production. To fully comprehend the dwarfing mechanism of DW871 and provide a theoretical foundation for future applications of the variety, we used transcriptome and proteome sequencing to identify genes and proteins associated with the dwarfing phenotype, using homologous high-stalk material HW871 as a control. By RNA-seq and iTRAQ, we discovered 8665 DEGs and 50 DAPs. Comprehensive transcription and translation level analysis revealed 25 correlations, 23 of which have the same expression trend, involving monolignin synthesis, pectin-lignin assembly, lignification, glucose modification, cell wall composition and architecture, cell morphology, vascular bundle development, and stalk tissue composition and architecture. As a result of these results, we can formulate a hypothesis about the DW871 dwarfing phenotype: plant hormone signal transduction, such as IAA and BRs, is linked to the formation of dwarf phenotypes, and metabolic pathways related to lignin synthesis, such as phenylpropane biosynthesis, also play a role. Our works will contribute to a better understanding of the genes and proteins involved in the rapeseed dwarf phenotype, and we will propose new insights into the dwarfing mechanism of Brassica napus L. MDPI 2022-02-02 /pmc/articles/PMC8838640/ /pubmed/35161394 http://dx.doi.org/10.3390/plants11030413 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Luo, Jing Huang, Sha Wang, Min Zhang, Ruimao Zhao, Degang Yang, Yuanyu Wang, Fang Wang, Zhuanzhuan Tang, Rong Wang, Lulu Xiao, Huagui Yang, Bin Li, Chao Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
title | Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
title_full | Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
title_fullStr | Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
title_full_unstemmed | Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
title_short | Characterization of the Transcriptome and Proteome of Brassica napus Reveals the Close Relation between DW871 Dwarfing Phenotype and Stalk Tissue |
title_sort | characterization of the transcriptome and proteome of brassica napus reveals the close relation between dw871 dwarfing phenotype and stalk tissue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838640/ https://www.ncbi.nlm.nih.gov/pubmed/35161394 http://dx.doi.org/10.3390/plants11030413 |
work_keys_str_mv | AT luojing characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT huangsha characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT wangmin characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT zhangruimao characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT zhaodegang characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT yangyuanyu characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT wangfang characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT wangzhuanzhuan characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT tangrong characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT wanglulu characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT xiaohuagui characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT yangbin characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue AT lichao characterizationofthetranscriptomeandproteomeofbrassicanapusrevealsthecloserelationbetweendw871dwarfingphenotypeandstalktissue |