LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz

Liquid chromatography electrospray ionization tandem mass spectrometric (LC-ESI-MS/MS) qualitative and quantitative analysis of different extracts from the aerial parts and roots of Alchemilla acutiloba led to the identification of phenolic acids and flavonoids. To the best of our knowledge, isorham...

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Autores principales: Dos Santos Szewczyk, Katarzyna, Pietrzak, Wioleta, Klimek, Katarzyna, Grzywa-Celińska, Anna, Celiński, Rafał, Gogacz, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838776/
https://www.ncbi.nlm.nih.gov/pubmed/35163884
http://dx.doi.org/10.3390/molecules27030621
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author Dos Santos Szewczyk, Katarzyna
Pietrzak, Wioleta
Klimek, Katarzyna
Grzywa-Celińska, Anna
Celiński, Rafał
Gogacz, Marek
author_facet Dos Santos Szewczyk, Katarzyna
Pietrzak, Wioleta
Klimek, Katarzyna
Grzywa-Celińska, Anna
Celiński, Rafał
Gogacz, Marek
author_sort Dos Santos Szewczyk, Katarzyna
collection PubMed
description Liquid chromatography electrospray ionization tandem mass spectrometric (LC-ESI-MS/MS) qualitative and quantitative analysis of different extracts from the aerial parts and roots of Alchemilla acutiloba led to the identification of phenolic acids and flavonoids. To the best of our knowledge, isorhamnetin 3-glucoside, kaempferol 3-rutinoside, narcissoside, naringenin 7-glucoside, 3-O-methylquercetin, naringenin, eriodictyol, rhamnetin, and isorhamnetin were described for the first time in Alchemilla genus. In addition, the antioxidant, anti-inflammatory and cytotoxic activity of all extracts were evaluated. The results clearly showed that among analyzed extracts, the butanol extract of the aerial parts exhibited the highest biological activity comparable with the positive controls used.
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spelling pubmed-88387762022-02-13 LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz Dos Santos Szewczyk, Katarzyna Pietrzak, Wioleta Klimek, Katarzyna Grzywa-Celińska, Anna Celiński, Rafał Gogacz, Marek Molecules Article Liquid chromatography electrospray ionization tandem mass spectrometric (LC-ESI-MS/MS) qualitative and quantitative analysis of different extracts from the aerial parts and roots of Alchemilla acutiloba led to the identification of phenolic acids and flavonoids. To the best of our knowledge, isorhamnetin 3-glucoside, kaempferol 3-rutinoside, narcissoside, naringenin 7-glucoside, 3-O-methylquercetin, naringenin, eriodictyol, rhamnetin, and isorhamnetin were described for the first time in Alchemilla genus. In addition, the antioxidant, anti-inflammatory and cytotoxic activity of all extracts were evaluated. The results clearly showed that among analyzed extracts, the butanol extract of the aerial parts exhibited the highest biological activity comparable with the positive controls used. MDPI 2022-01-18 /pmc/articles/PMC8838776/ /pubmed/35163884 http://dx.doi.org/10.3390/molecules27030621 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dos Santos Szewczyk, Katarzyna
Pietrzak, Wioleta
Klimek, Katarzyna
Grzywa-Celińska, Anna
Celiński, Rafał
Gogacz, Marek
LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz
title LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz
title_full LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz
title_fullStr LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz
title_full_unstemmed LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz
title_short LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz
title_sort lc-esi-ms/ms identification of biologically active phenolics in different extracts of alchemilla acutiloba opiz
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838776/
https://www.ncbi.nlm.nih.gov/pubmed/35163884
http://dx.doi.org/10.3390/molecules27030621
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