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Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong”

Overaccumulation of lignin (a physiological disorder known as granulation) often occurs during fruit ripening and postharvest storage in pomelo (Citrus grandis). It causes an unpleasant fruit texture and taste. Previous studies have shown that lignin metabolism is closely associated with the process...

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Autores principales: Li, Xiaoting, Wang, Naiyu, She, Wenqin, Guo, Zhixiong, Pan, Heli, Yu, Yuan, Ye, Jianwen, Pan, Dongming, Pan, Tengfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838788/
https://www.ncbi.nlm.nih.gov/pubmed/35161384
http://dx.doi.org/10.3390/plants11030403
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author Li, Xiaoting
Wang, Naiyu
She, Wenqin
Guo, Zhixiong
Pan, Heli
Yu, Yuan
Ye, Jianwen
Pan, Dongming
Pan, Tengfei
author_facet Li, Xiaoting
Wang, Naiyu
She, Wenqin
Guo, Zhixiong
Pan, Heli
Yu, Yuan
Ye, Jianwen
Pan, Dongming
Pan, Tengfei
author_sort Li, Xiaoting
collection PubMed
description Overaccumulation of lignin (a physiological disorder known as granulation) often occurs during fruit ripening and postharvest storage in pomelo (Citrus grandis). It causes an unpleasant fruit texture and taste. Previous studies have shown that lignin metabolism is closely associated with the process of juice sacs granulation. At present, the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a candidate NAC transcription factor, CgNAC043, that is involved in the regulation of lignin biosynthesis in Citrus grandis, which has homologs in Arabidopsis and other plants. We used the fruit juice sacs of ‘San hong’ as the material, the staining for lignin with HCl−phloroglucinol of fruit juice sacs became dark red from the various developmental stages at 172 to 212 days post anthesis (DPA). The RT-qPCR was used to analyze the gene expression of CgNAC043 and its target gene CgMYB46 in fruit sacs, it was found that the expression trend of CgNAC043 was basically same as CgMYB46, which increased gradually and peaked at 212 DPA. The expression level of CgNAC043 in juice sacs obtained away from the core was the lowest, while those near the core and granulated area were highly expressed. The transcriptional activation activity of CgNAC043 and CgMYB46 was analyzed by a yeast two-hybrid system, with only CgNAC043 showing transcriptional activation activity in Y2H Gold yeast. A transformation vector, p1301- CgNAC043, was transformed into the mesocarp of ‘San hong’ by Agrobacterium-mediated transformation. Results showed that the expression of transcription factors CgMYB58 and CgMYB46 are all upregulated. Further experiments proved that CgNAC043 not only can directly trans-activate the promoter of CgMYB46 but also trans-activate the promoters for the lignin biosynthesis-related genes CgCCoAOMT and CgC3H by dual luciferase assay. We isolated the CgNAC043 gene in pomelo and found CgNAC043 regulates target genes conferring the regulation of juice sacs granulation.
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spelling pubmed-88387882022-02-13 Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong” Li, Xiaoting Wang, Naiyu She, Wenqin Guo, Zhixiong Pan, Heli Yu, Yuan Ye, Jianwen Pan, Dongming Pan, Tengfei Plants (Basel) Article Overaccumulation of lignin (a physiological disorder known as granulation) often occurs during fruit ripening and postharvest storage in pomelo (Citrus grandis). It causes an unpleasant fruit texture and taste. Previous studies have shown that lignin metabolism is closely associated with the process of juice sacs granulation. At present, the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a candidate NAC transcription factor, CgNAC043, that is involved in the regulation of lignin biosynthesis in Citrus grandis, which has homologs in Arabidopsis and other plants. We used the fruit juice sacs of ‘San hong’ as the material, the staining for lignin with HCl−phloroglucinol of fruit juice sacs became dark red from the various developmental stages at 172 to 212 days post anthesis (DPA). The RT-qPCR was used to analyze the gene expression of CgNAC043 and its target gene CgMYB46 in fruit sacs, it was found that the expression trend of CgNAC043 was basically same as CgMYB46, which increased gradually and peaked at 212 DPA. The expression level of CgNAC043 in juice sacs obtained away from the core was the lowest, while those near the core and granulated area were highly expressed. The transcriptional activation activity of CgNAC043 and CgMYB46 was analyzed by a yeast two-hybrid system, with only CgNAC043 showing transcriptional activation activity in Y2H Gold yeast. A transformation vector, p1301- CgNAC043, was transformed into the mesocarp of ‘San hong’ by Agrobacterium-mediated transformation. Results showed that the expression of transcription factors CgMYB58 and CgMYB46 are all upregulated. Further experiments proved that CgNAC043 not only can directly trans-activate the promoter of CgMYB46 but also trans-activate the promoters for the lignin biosynthesis-related genes CgCCoAOMT and CgC3H by dual luciferase assay. We isolated the CgNAC043 gene in pomelo and found CgNAC043 regulates target genes conferring the regulation of juice sacs granulation. MDPI 2022-01-31 /pmc/articles/PMC8838788/ /pubmed/35161384 http://dx.doi.org/10.3390/plants11030403 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Xiaoting
Wang, Naiyu
She, Wenqin
Guo, Zhixiong
Pan, Heli
Yu, Yuan
Ye, Jianwen
Pan, Dongming
Pan, Tengfei
Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong”
title Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong”
title_full Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong”
title_fullStr Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong”
title_full_unstemmed Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong”
title_short Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong”
title_sort identification and functional analysis of the cgnac043 gene involved in lignin synthesis from citrus grandis “san hong”
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838788/
https://www.ncbi.nlm.nih.gov/pubmed/35161384
http://dx.doi.org/10.3390/plants11030403
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