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Evaluation of the Potential of a Lectin Extracted from Polygonum persicaria L. as a Biorational Agent against Sitophilus oryzae L.

Rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae), is one of the most destructive stored-product pests that is resistant to a wide range of chemical insecticides. In the present study, we investigated whether a lectin extracted from Polygonum persicaria L. (PPA) can be used as a bioratio...

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Autores principales: Khoobdel, Mehdi, Rahimi, Vahid, Ebadollahi, Asgar, Krutmuang, Patcharin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838870/
https://www.ncbi.nlm.nih.gov/pubmed/35164055
http://dx.doi.org/10.3390/molecules27030793
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author Khoobdel, Mehdi
Rahimi, Vahid
Ebadollahi, Asgar
Krutmuang, Patcharin
author_facet Khoobdel, Mehdi
Rahimi, Vahid
Ebadollahi, Asgar
Krutmuang, Patcharin
author_sort Khoobdel, Mehdi
collection PubMed
description Rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae), is one of the most destructive stored-product pests that is resistant to a wide range of chemical insecticides. In the present study, we investigated whether a lectin extracted from Polygonum persicaria L. (PPA) can be used as a biorational agent to control such insect pests. Along with the lethal digestive assay, the sub-lethal insecticidal activities of PPA, including the effects on digestive, detoxifying, and antioxidant enzyme activities, were evaluated against S. oryzae adults. The effect of feeding a diet containing PPA and carob extract as a food attractant on the mortality of S. oryzae adults was also investigated. Feeding on the diet containing PPA resulted in a significant mortality of S. oryzae adults with a LC(50) (Lethal Concentration to kill 50% of insects) of 3.68% (w/w). The activity of digestive enzymes, including α-amylase, α-glucosidase, TAG-lipase, trypsin, chymotrypsin, elastase, and carboxy- and aminopeptidase, were decreased by the sub-lethal concentration of PPA. Detoxifying and antioxidant enzymes, including esterase, superoxide dismutase, catalase, glutathione-S-transferase, ascorbate peroxidase, glucose 6-phosphate dehydrogenase, and malondialdehyde, were activated in adults affected by PPA. These findings indicated that PPA, in addition to causing digestive disorders, leads to oxidative stress in S. oryzae. The presence of carob extract had no effect on the PPA-induced mortality of the insect. According to the results of the present study, PPA has promising insecticidal efficiency against S. oryzae. In addition, the usage of PPA with a food attractant carob extract in bait traps can be recommended as a new biorational formulation in S. oryzae management.
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spelling pubmed-88388702022-02-13 Evaluation of the Potential of a Lectin Extracted from Polygonum persicaria L. as a Biorational Agent against Sitophilus oryzae L. Khoobdel, Mehdi Rahimi, Vahid Ebadollahi, Asgar Krutmuang, Patcharin Molecules Article Rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae), is one of the most destructive stored-product pests that is resistant to a wide range of chemical insecticides. In the present study, we investigated whether a lectin extracted from Polygonum persicaria L. (PPA) can be used as a biorational agent to control such insect pests. Along with the lethal digestive assay, the sub-lethal insecticidal activities of PPA, including the effects on digestive, detoxifying, and antioxidant enzyme activities, were evaluated against S. oryzae adults. The effect of feeding a diet containing PPA and carob extract as a food attractant on the mortality of S. oryzae adults was also investigated. Feeding on the diet containing PPA resulted in a significant mortality of S. oryzae adults with a LC(50) (Lethal Concentration to kill 50% of insects) of 3.68% (w/w). The activity of digestive enzymes, including α-amylase, α-glucosidase, TAG-lipase, trypsin, chymotrypsin, elastase, and carboxy- and aminopeptidase, were decreased by the sub-lethal concentration of PPA. Detoxifying and antioxidant enzymes, including esterase, superoxide dismutase, catalase, glutathione-S-transferase, ascorbate peroxidase, glucose 6-phosphate dehydrogenase, and malondialdehyde, were activated in adults affected by PPA. These findings indicated that PPA, in addition to causing digestive disorders, leads to oxidative stress in S. oryzae. The presence of carob extract had no effect on the PPA-induced mortality of the insect. According to the results of the present study, PPA has promising insecticidal efficiency against S. oryzae. In addition, the usage of PPA with a food attractant carob extract in bait traps can be recommended as a new biorational formulation in S. oryzae management. MDPI 2022-01-25 /pmc/articles/PMC8838870/ /pubmed/35164055 http://dx.doi.org/10.3390/molecules27030793 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khoobdel, Mehdi
Rahimi, Vahid
Ebadollahi, Asgar
Krutmuang, Patcharin
Evaluation of the Potential of a Lectin Extracted from Polygonum persicaria L. as a Biorational Agent against Sitophilus oryzae L.
title Evaluation of the Potential of a Lectin Extracted from Polygonum persicaria L. as a Biorational Agent against Sitophilus oryzae L.
title_full Evaluation of the Potential of a Lectin Extracted from Polygonum persicaria L. as a Biorational Agent against Sitophilus oryzae L.
title_fullStr Evaluation of the Potential of a Lectin Extracted from Polygonum persicaria L. as a Biorational Agent against Sitophilus oryzae L.
title_full_unstemmed Evaluation of the Potential of a Lectin Extracted from Polygonum persicaria L. as a Biorational Agent against Sitophilus oryzae L.
title_short Evaluation of the Potential of a Lectin Extracted from Polygonum persicaria L. as a Biorational Agent against Sitophilus oryzae L.
title_sort evaluation of the potential of a lectin extracted from polygonum persicaria l. as a biorational agent against sitophilus oryzae l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838870/
https://www.ncbi.nlm.nih.gov/pubmed/35164055
http://dx.doi.org/10.3390/molecules27030793
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