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Combining Hyperspectral Reflectance Indices and Multivariate Analysis to Estimate Different Units of Chlorophyll Content of Spring Wheat under Salinity Conditions

Although plant chlorophyll (Chl) is one of the important elements in monitoring plant stress and reflects the photosynthetic capacity of plants, their measurement in the lab is generally time- and cost-inefficient and based on a small part of the leaf. This study examines the ability of canopy spect...

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Autores principales: El-Hendawy, Salah, Dewir, Yaser Hassan, Elsayed, Salah, Schmidhalter, Urs, Al-Gaadi, Khalid, Tola, ElKamil, Refay, Yahya, Tahir, Muhammad Usman, Hassan, Wael M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839343/
https://www.ncbi.nlm.nih.gov/pubmed/35161437
http://dx.doi.org/10.3390/plants11030456
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author El-Hendawy, Salah
Dewir, Yaser Hassan
Elsayed, Salah
Schmidhalter, Urs
Al-Gaadi, Khalid
Tola, ElKamil
Refay, Yahya
Tahir, Muhammad Usman
Hassan, Wael M.
author_facet El-Hendawy, Salah
Dewir, Yaser Hassan
Elsayed, Salah
Schmidhalter, Urs
Al-Gaadi, Khalid
Tola, ElKamil
Refay, Yahya
Tahir, Muhammad Usman
Hassan, Wael M.
author_sort El-Hendawy, Salah
collection PubMed
description Although plant chlorophyll (Chl) is one of the important elements in monitoring plant stress and reflects the photosynthetic capacity of plants, their measurement in the lab is generally time- and cost-inefficient and based on a small part of the leaf. This study examines the ability of canopy spectral reflectance data for the accurate estimation of the Chl content of two wheat genotypes grown under three salinity levels. The Chl content was quantified as content per area (Chl (area), μg cm(−2)), concentration per plant (Chl (plant), mg plant(−1)), and SPAD value (Chl (SPAD)). The performance of spectral reflectance indices (SRIs) with different algorithm forms, partial least square regression (PLSR), and stepwise multiple linear regression (SMLR) in estimating the three units of Chl content was compared. Results show that most indices within each SRI form performed better with Chl (area) and Chl (plant) and performed poorly with Chl (SPAD). The PLSR models, based on the four forms of SRIs individually or combined, still performed poorly in estimating Chl (SPAD), while they exhibited a strong relationship with Chl (plant) followed by Chl (area) in both the calibration (Cal.) and validation (Val.) datasets. The SMLR models extracted three to four indices from each SRI form as the most effective indices and explained 73–79%, 80–84%, and 39–43% of the total variability in Chl (area), Chl (plant), and Chl (SPAD), respectively. The performance of the various predictive models of SMLR for predicting Chl content depended on salinity level, genotype, season, and the units of Chl content. In summary, this study indicates that the Chl content measured in the lab and expressed on content (μg cm(−2)) or concentration (mg plant(−1)) can be accurately estimated at canopy level using spectral reflectance data.
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spelling pubmed-88393432022-02-13 Combining Hyperspectral Reflectance Indices and Multivariate Analysis to Estimate Different Units of Chlorophyll Content of Spring Wheat under Salinity Conditions El-Hendawy, Salah Dewir, Yaser Hassan Elsayed, Salah Schmidhalter, Urs Al-Gaadi, Khalid Tola, ElKamil Refay, Yahya Tahir, Muhammad Usman Hassan, Wael M. Plants (Basel) Article Although plant chlorophyll (Chl) is one of the important elements in monitoring plant stress and reflects the photosynthetic capacity of plants, their measurement in the lab is generally time- and cost-inefficient and based on a small part of the leaf. This study examines the ability of canopy spectral reflectance data for the accurate estimation of the Chl content of two wheat genotypes grown under three salinity levels. The Chl content was quantified as content per area (Chl (area), μg cm(−2)), concentration per plant (Chl (plant), mg plant(−1)), and SPAD value (Chl (SPAD)). The performance of spectral reflectance indices (SRIs) with different algorithm forms, partial least square regression (PLSR), and stepwise multiple linear regression (SMLR) in estimating the three units of Chl content was compared. Results show that most indices within each SRI form performed better with Chl (area) and Chl (plant) and performed poorly with Chl (SPAD). The PLSR models, based on the four forms of SRIs individually or combined, still performed poorly in estimating Chl (SPAD), while they exhibited a strong relationship with Chl (plant) followed by Chl (area) in both the calibration (Cal.) and validation (Val.) datasets. The SMLR models extracted three to four indices from each SRI form as the most effective indices and explained 73–79%, 80–84%, and 39–43% of the total variability in Chl (area), Chl (plant), and Chl (SPAD), respectively. The performance of the various predictive models of SMLR for predicting Chl content depended on salinity level, genotype, season, and the units of Chl content. In summary, this study indicates that the Chl content measured in the lab and expressed on content (μg cm(−2)) or concentration (mg plant(−1)) can be accurately estimated at canopy level using spectral reflectance data. MDPI 2022-02-07 /pmc/articles/PMC8839343/ /pubmed/35161437 http://dx.doi.org/10.3390/plants11030456 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
El-Hendawy, Salah
Dewir, Yaser Hassan
Elsayed, Salah
Schmidhalter, Urs
Al-Gaadi, Khalid
Tola, ElKamil
Refay, Yahya
Tahir, Muhammad Usman
Hassan, Wael M.
Combining Hyperspectral Reflectance Indices and Multivariate Analysis to Estimate Different Units of Chlorophyll Content of Spring Wheat under Salinity Conditions
title Combining Hyperspectral Reflectance Indices and Multivariate Analysis to Estimate Different Units of Chlorophyll Content of Spring Wheat under Salinity Conditions
title_full Combining Hyperspectral Reflectance Indices and Multivariate Analysis to Estimate Different Units of Chlorophyll Content of Spring Wheat under Salinity Conditions
title_fullStr Combining Hyperspectral Reflectance Indices and Multivariate Analysis to Estimate Different Units of Chlorophyll Content of Spring Wheat under Salinity Conditions
title_full_unstemmed Combining Hyperspectral Reflectance Indices and Multivariate Analysis to Estimate Different Units of Chlorophyll Content of Spring Wheat under Salinity Conditions
title_short Combining Hyperspectral Reflectance Indices and Multivariate Analysis to Estimate Different Units of Chlorophyll Content of Spring Wheat under Salinity Conditions
title_sort combining hyperspectral reflectance indices and multivariate analysis to estimate different units of chlorophyll content of spring wheat under salinity conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839343/
https://www.ncbi.nlm.nih.gov/pubmed/35161437
http://dx.doi.org/10.3390/plants11030456
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