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Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release

Hypoalbuminemia can lead to poor and delayed wound healing, while it is also associated with acute myocardial infarction, heart failure, malignancies, and COVID-19. In elective surgery, patients with low albumin have high risks of postoperative wound complications. Here, we propose a novel cost-effe...

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Autores principales: Kocaaga, Banu, Kurkcuoglu, Ozge, Tatlier, Melkon, Dinler-Doganay, Gizem, Batirel, Saime, Güner, Fatma Seniha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839484/
https://www.ncbi.nlm.nih.gov/pubmed/35160450
http://dx.doi.org/10.3390/polym14030460
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author Kocaaga, Banu
Kurkcuoglu, Ozge
Tatlier, Melkon
Dinler-Doganay, Gizem
Batirel, Saime
Güner, Fatma Seniha
author_facet Kocaaga, Banu
Kurkcuoglu, Ozge
Tatlier, Melkon
Dinler-Doganay, Gizem
Batirel, Saime
Güner, Fatma Seniha
author_sort Kocaaga, Banu
collection PubMed
description Hypoalbuminemia can lead to poor and delayed wound healing, while it is also associated with acute myocardial infarction, heart failure, malignancies, and COVID-19. In elective surgery, patients with low albumin have high risks of postoperative wound complications. Here, we propose a novel cost-effective wound dressing material based on low-methoxy pectin and NaA-zeolite particles with controlled albumin release properties. We focused on both albumin adsorption and release phenomena for wounds with excess exudate. Firstly, we investigated albumin dynamics and calculated electrostatic surfaces at experimental pH values in water by using molecular dynamics methods. Then, we studied in detail pectin–zeolite hydrogels with both adsorption and diffusion into membrane methods using different pH values and albumin concentrations. To understand if uploaded albumin molecules preserved their secondary conformation in different formulations, we monitored the effect of pH and albumin concentration on the conformational changes in albumin after it was released from the hydrogels by using CD-UV spectroscopy analyses. Our results indicate that at pH 6.4, BSA-containing films preserved the protein’s folded structure while the protein was being released to the external buffer solutions. In vitro wound healing assay indicated that albumin-loaded hydrogels showed no toxic effects on the fibroblast cells.
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spelling pubmed-88394842022-02-13 Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release Kocaaga, Banu Kurkcuoglu, Ozge Tatlier, Melkon Dinler-Doganay, Gizem Batirel, Saime Güner, Fatma Seniha Polymers (Basel) Article Hypoalbuminemia can lead to poor and delayed wound healing, while it is also associated with acute myocardial infarction, heart failure, malignancies, and COVID-19. In elective surgery, patients with low albumin have high risks of postoperative wound complications. Here, we propose a novel cost-effective wound dressing material based on low-methoxy pectin and NaA-zeolite particles with controlled albumin release properties. We focused on both albumin adsorption and release phenomena for wounds with excess exudate. Firstly, we investigated albumin dynamics and calculated electrostatic surfaces at experimental pH values in water by using molecular dynamics methods. Then, we studied in detail pectin–zeolite hydrogels with both adsorption and diffusion into membrane methods using different pH values and albumin concentrations. To understand if uploaded albumin molecules preserved their secondary conformation in different formulations, we monitored the effect of pH and albumin concentration on the conformational changes in albumin after it was released from the hydrogels by using CD-UV spectroscopy analyses. Our results indicate that at pH 6.4, BSA-containing films preserved the protein’s folded structure while the protein was being released to the external buffer solutions. In vitro wound healing assay indicated that albumin-loaded hydrogels showed no toxic effects on the fibroblast cells. MDPI 2022-01-24 /pmc/articles/PMC8839484/ /pubmed/35160450 http://dx.doi.org/10.3390/polym14030460 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kocaaga, Banu
Kurkcuoglu, Ozge
Tatlier, Melkon
Dinler-Doganay, Gizem
Batirel, Saime
Güner, Fatma Seniha
Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release
title Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release
title_full Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release
title_fullStr Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release
title_full_unstemmed Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release
title_short Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release
title_sort pectin–zeolite-based wound dressings with controlled albumin release
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839484/
https://www.ncbi.nlm.nih.gov/pubmed/35160450
http://dx.doi.org/10.3390/polym14030460
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