Expression and Surface Display of an Acidic Cold-Active Chitosanase in Pichia pastoris Using Multi-Copy Expression and High-Density Cultivation

Chitosanase hydrolyzes β-(1,4)-linked glycosidic bonds are used in chitosan chains to release oligosaccharide mixtures. Here, we cloned and expressed a cold-adapted chitosanase (CDA, Genbank: MW094131) using multi-copy expression plasmids (CDA1/2/3/4) in Pichia pastoris. We identified elevated CDA e...

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Detalles Bibliográficos
Autores principales: Peng, Yanhong, Wang, Yaping, Liu, Xiaoyan, Zhou, Ronghua, Liao, Xianqing, Min, Yong, Ma, Lixin, Wang, Ying, Rao, Ben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839494/
https://www.ncbi.nlm.nih.gov/pubmed/35164064
http://dx.doi.org/10.3390/molecules27030800

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839494/
https://www.ncbi.nlm.nih.gov/pubmed/35164064
http://dx.doi.org/10.3390/molecules27030800

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