Cargando…

Development of Novel Detection Method for Rutinosidase in Tartary Buckwheat (Fagopyrum tataricum Gaertn.)

Contamination of rutinosidase is a major problem for rutin-rich food due to the hydrolysis of the functional compound rutin and generation of strong bitterness caused by the hydrolyzed moiety quercetin. This problem sometimes occurs between the trace and normal rutinosidase Tartary buckwheat varieti...

Descripción completa

Detalles Bibliográficos
Autores principales: Suzuki, Tatsuro, Morishita, Toshikazu, Takigawa, Shigenobu, Noda, Takahiro, Ishiguro, Koji, Otsuka, Shiori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839646/
https://www.ncbi.nlm.nih.gov/pubmed/35161301
http://dx.doi.org/10.3390/plants11030320
_version_ 1784650419302039552
author Suzuki, Tatsuro
Morishita, Toshikazu
Takigawa, Shigenobu
Noda, Takahiro
Ishiguro, Koji
Otsuka, Shiori
author_facet Suzuki, Tatsuro
Morishita, Toshikazu
Takigawa, Shigenobu
Noda, Takahiro
Ishiguro, Koji
Otsuka, Shiori
author_sort Suzuki, Tatsuro
collection PubMed
description Contamination of rutinosidase is a major problem for rutin-rich food due to the hydrolysis of the functional compound rutin and generation of strong bitterness caused by the hydrolyzed moiety quercetin. This problem sometimes occurs between the trace and normal rutinosidase Tartary buckwheat varieties. Here, we developed a simple and rapid method for rutinosidase detection in ‘ripening seeds using UV light’ and in ‘dough using alum-flavonoid complexation’ from Tartary buckwheat (Fagopyrum tataricum Gaertn.). Normal rutinosidase seeds can be distinguished from trace-rutinosidase mature seeds and ripening young seeds using a rutin solution by comparing the muddiness, which corresponds to quercetin generation. In dough, we detected a threefold relative increase in rutinosidase activity corresponding to 1% contamination of normal rutinosidase flour with trace-rutinosidase flour within 65 min. These methods do not require expensive apparatuses and toxic chemicals and are therefore promising for detecting and preventing contamination by rutinosidase, e.g., in food processing factories.
format Online
Article
Text
id pubmed-8839646
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88396462022-02-13 Development of Novel Detection Method for Rutinosidase in Tartary Buckwheat (Fagopyrum tataricum Gaertn.) Suzuki, Tatsuro Morishita, Toshikazu Takigawa, Shigenobu Noda, Takahiro Ishiguro, Koji Otsuka, Shiori Plants (Basel) Communication Contamination of rutinosidase is a major problem for rutin-rich food due to the hydrolysis of the functional compound rutin and generation of strong bitterness caused by the hydrolyzed moiety quercetin. This problem sometimes occurs between the trace and normal rutinosidase Tartary buckwheat varieties. Here, we developed a simple and rapid method for rutinosidase detection in ‘ripening seeds using UV light’ and in ‘dough using alum-flavonoid complexation’ from Tartary buckwheat (Fagopyrum tataricum Gaertn.). Normal rutinosidase seeds can be distinguished from trace-rutinosidase mature seeds and ripening young seeds using a rutin solution by comparing the muddiness, which corresponds to quercetin generation. In dough, we detected a threefold relative increase in rutinosidase activity corresponding to 1% contamination of normal rutinosidase flour with trace-rutinosidase flour within 65 min. These methods do not require expensive apparatuses and toxic chemicals and are therefore promising for detecting and preventing contamination by rutinosidase, e.g., in food processing factories. MDPI 2022-01-25 /pmc/articles/PMC8839646/ /pubmed/35161301 http://dx.doi.org/10.3390/plants11030320 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Suzuki, Tatsuro
Morishita, Toshikazu
Takigawa, Shigenobu
Noda, Takahiro
Ishiguro, Koji
Otsuka, Shiori
Development of Novel Detection Method for Rutinosidase in Tartary Buckwheat (Fagopyrum tataricum Gaertn.)
title Development of Novel Detection Method for Rutinosidase in Tartary Buckwheat (Fagopyrum tataricum Gaertn.)
title_full Development of Novel Detection Method for Rutinosidase in Tartary Buckwheat (Fagopyrum tataricum Gaertn.)
title_fullStr Development of Novel Detection Method for Rutinosidase in Tartary Buckwheat (Fagopyrum tataricum Gaertn.)
title_full_unstemmed Development of Novel Detection Method for Rutinosidase in Tartary Buckwheat (Fagopyrum tataricum Gaertn.)
title_short Development of Novel Detection Method for Rutinosidase in Tartary Buckwheat (Fagopyrum tataricum Gaertn.)
title_sort development of novel detection method for rutinosidase in tartary buckwheat (fagopyrum tataricum gaertn.)
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8839646/
https://www.ncbi.nlm.nih.gov/pubmed/35161301
http://dx.doi.org/10.3390/plants11030320
work_keys_str_mv AT suzukitatsuro developmentofnoveldetectionmethodforrutinosidaseintartarybuckwheatfagopyrumtataricumgaertn
AT morishitatoshikazu developmentofnoveldetectionmethodforrutinosidaseintartarybuckwheatfagopyrumtataricumgaertn
AT takigawashigenobu developmentofnoveldetectionmethodforrutinosidaseintartarybuckwheatfagopyrumtataricumgaertn
AT nodatakahiro developmentofnoveldetectionmethodforrutinosidaseintartarybuckwheatfagopyrumtataricumgaertn
AT ishigurokoji developmentofnoveldetectionmethodforrutinosidaseintartarybuckwheatfagopyrumtataricumgaertn
AT otsukashiori developmentofnoveldetectionmethodforrutinosidaseintartarybuckwheatfagopyrumtataricumgaertn