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The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles

In recent years, silk fibroin nanoparticles (SFNs) have been consolidated as drug delivery systems (DDSs) with multiple applications in personalized medicine. The design of a simple, inexpensive, and scalable preparation method is an objective pursued by many research groups. When the objective is t...

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Autores principales: Asensio Ruiz, María Alejandra, Fuster, Marta G., Martínez Martínez, Teresa, Montalbán, Mercedes G., Cenis, José Luis, Víllora, Gloria, Lozano-Pérez, Antonio Abel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840090/
https://www.ncbi.nlm.nih.gov/pubmed/35160487
http://dx.doi.org/10.3390/polym14030498
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author Asensio Ruiz, María Alejandra
Fuster, Marta G.
Martínez Martínez, Teresa
Montalbán, Mercedes G.
Cenis, José Luis
Víllora, Gloria
Lozano-Pérez, Antonio Abel
author_facet Asensio Ruiz, María Alejandra
Fuster, Marta G.
Martínez Martínez, Teresa
Montalbán, Mercedes G.
Cenis, José Luis
Víllora, Gloria
Lozano-Pérez, Antonio Abel
author_sort Asensio Ruiz, María Alejandra
collection PubMed
description In recent years, silk fibroin nanoparticles (SFNs) have been consolidated as drug delivery systems (DDSs) with multiple applications in personalized medicine. The design of a simple, inexpensive, and scalable preparation method is an objective pursued by many research groups. When the objective is to produce nanoparticles suitable for biomedical uses, their sterility is essential. To achieve sufficient control of all the crucial stages in the process and knowledge of their implications for the final characteristics of the nanoparticles, the present work focused on the final stage of sterilization. In this work, the sterilization of SFNs was studied by comparing the effect of different available treatments on the characteristics of the nanoparticles. Two different sterilization methods, gamma irradiation and autoclaving, were tested, and optimal conditions were identified to achieve the sterilization of SFNs by gamma irradiation. The minimum irradiation dose to achieve sterilization of the nanoparticle suspension without changes in the nanoparticle size, polydispersity, or Z-potential was determined to be 5 kiloGrays (kGy). These simple and safe methods were successfully implemented for the sterilization of SFNs in aqueous suspension and facilitate the application of these nanoparticles in medicine.
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spelling pubmed-88400902022-02-13 The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles Asensio Ruiz, María Alejandra Fuster, Marta G. Martínez Martínez, Teresa Montalbán, Mercedes G. Cenis, José Luis Víllora, Gloria Lozano-Pérez, Antonio Abel Polymers (Basel) Article In recent years, silk fibroin nanoparticles (SFNs) have been consolidated as drug delivery systems (DDSs) with multiple applications in personalized medicine. The design of a simple, inexpensive, and scalable preparation method is an objective pursued by many research groups. When the objective is to produce nanoparticles suitable for biomedical uses, their sterility is essential. To achieve sufficient control of all the crucial stages in the process and knowledge of their implications for the final characteristics of the nanoparticles, the present work focused on the final stage of sterilization. In this work, the sterilization of SFNs was studied by comparing the effect of different available treatments on the characteristics of the nanoparticles. Two different sterilization methods, gamma irradiation and autoclaving, were tested, and optimal conditions were identified to achieve the sterilization of SFNs by gamma irradiation. The minimum irradiation dose to achieve sterilization of the nanoparticle suspension without changes in the nanoparticle size, polydispersity, or Z-potential was determined to be 5 kiloGrays (kGy). These simple and safe methods were successfully implemented for the sterilization of SFNs in aqueous suspension and facilitate the application of these nanoparticles in medicine. MDPI 2022-01-26 /pmc/articles/PMC8840090/ /pubmed/35160487 http://dx.doi.org/10.3390/polym14030498 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Asensio Ruiz, María Alejandra
Fuster, Marta G.
Martínez Martínez, Teresa
Montalbán, Mercedes G.
Cenis, José Luis
Víllora, Gloria
Lozano-Pérez, Antonio Abel
The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles
title The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles
title_full The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles
title_fullStr The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles
title_full_unstemmed The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles
title_short The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles
title_sort effect of sterilization on the characteristics of silk fibroin nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840090/
https://www.ncbi.nlm.nih.gov/pubmed/35160487
http://dx.doi.org/10.3390/polym14030498
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