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The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles
In recent years, silk fibroin nanoparticles (SFNs) have been consolidated as drug delivery systems (DDSs) with multiple applications in personalized medicine. The design of a simple, inexpensive, and scalable preparation method is an objective pursued by many research groups. When the objective is t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840090/ https://www.ncbi.nlm.nih.gov/pubmed/35160487 http://dx.doi.org/10.3390/polym14030498 |
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author | Asensio Ruiz, María Alejandra Fuster, Marta G. Martínez Martínez, Teresa Montalbán, Mercedes G. Cenis, José Luis Víllora, Gloria Lozano-Pérez, Antonio Abel |
author_facet | Asensio Ruiz, María Alejandra Fuster, Marta G. Martínez Martínez, Teresa Montalbán, Mercedes G. Cenis, José Luis Víllora, Gloria Lozano-Pérez, Antonio Abel |
author_sort | Asensio Ruiz, María Alejandra |
collection | PubMed |
description | In recent years, silk fibroin nanoparticles (SFNs) have been consolidated as drug delivery systems (DDSs) with multiple applications in personalized medicine. The design of a simple, inexpensive, and scalable preparation method is an objective pursued by many research groups. When the objective is to produce nanoparticles suitable for biomedical uses, their sterility is essential. To achieve sufficient control of all the crucial stages in the process and knowledge of their implications for the final characteristics of the nanoparticles, the present work focused on the final stage of sterilization. In this work, the sterilization of SFNs was studied by comparing the effect of different available treatments on the characteristics of the nanoparticles. Two different sterilization methods, gamma irradiation and autoclaving, were tested, and optimal conditions were identified to achieve the sterilization of SFNs by gamma irradiation. The minimum irradiation dose to achieve sterilization of the nanoparticle suspension without changes in the nanoparticle size, polydispersity, or Z-potential was determined to be 5 kiloGrays (kGy). These simple and safe methods were successfully implemented for the sterilization of SFNs in aqueous suspension and facilitate the application of these nanoparticles in medicine. |
format | Online Article Text |
id | pubmed-8840090 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88400902022-02-13 The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles Asensio Ruiz, María Alejandra Fuster, Marta G. Martínez Martínez, Teresa Montalbán, Mercedes G. Cenis, José Luis Víllora, Gloria Lozano-Pérez, Antonio Abel Polymers (Basel) Article In recent years, silk fibroin nanoparticles (SFNs) have been consolidated as drug delivery systems (DDSs) with multiple applications in personalized medicine. The design of a simple, inexpensive, and scalable preparation method is an objective pursued by many research groups. When the objective is to produce nanoparticles suitable for biomedical uses, their sterility is essential. To achieve sufficient control of all the crucial stages in the process and knowledge of their implications for the final characteristics of the nanoparticles, the present work focused on the final stage of sterilization. In this work, the sterilization of SFNs was studied by comparing the effect of different available treatments on the characteristics of the nanoparticles. Two different sterilization methods, gamma irradiation and autoclaving, were tested, and optimal conditions were identified to achieve the sterilization of SFNs by gamma irradiation. The minimum irradiation dose to achieve sterilization of the nanoparticle suspension without changes in the nanoparticle size, polydispersity, or Z-potential was determined to be 5 kiloGrays (kGy). These simple and safe methods were successfully implemented for the sterilization of SFNs in aqueous suspension and facilitate the application of these nanoparticles in medicine. MDPI 2022-01-26 /pmc/articles/PMC8840090/ /pubmed/35160487 http://dx.doi.org/10.3390/polym14030498 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Asensio Ruiz, María Alejandra Fuster, Marta G. Martínez Martínez, Teresa Montalbán, Mercedes G. Cenis, José Luis Víllora, Gloria Lozano-Pérez, Antonio Abel The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles |
title | The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles |
title_full | The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles |
title_fullStr | The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles |
title_full_unstemmed | The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles |
title_short | The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles |
title_sort | effect of sterilization on the characteristics of silk fibroin nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840090/ https://www.ncbi.nlm.nih.gov/pubmed/35160487 http://dx.doi.org/10.3390/polym14030498 |
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