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Total Phenolic Content and Antioxidant and Antimicrobial Activities of Papaver rhoeas L. Organ Extracts Growing in Taounate Region, Morocco

The objective of this study is to valorize Papaver rhoeas L. from the Taounate region of Morocco by determining the total polyphenol content (TPC), the total flavonoid content (TFC) and the antioxidant and antimicrobial activities of four organs. The quantification of TPC and TFC in root, stem, leaf...

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Detalles Bibliográficos
Autores principales: Hmamou, Anouar, Eloutassi, Noureddine, Alshawwa, Samar Zuhair, Al kamaly, Omkulthom, Kara, Mohammed, Bendaoud, Ahmed, El-Assri, El-Mehdi, Tlemcani, Sara, El Khomsi, Mostafa, Lahkimi, Amal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840091/
https://www.ncbi.nlm.nih.gov/pubmed/35164114
http://dx.doi.org/10.3390/molecules27030854
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author Hmamou, Anouar
Eloutassi, Noureddine
Alshawwa, Samar Zuhair
Al kamaly, Omkulthom
Kara, Mohammed
Bendaoud, Ahmed
El-Assri, El-Mehdi
Tlemcani, Sara
El Khomsi, Mostafa
Lahkimi, Amal
author_facet Hmamou, Anouar
Eloutassi, Noureddine
Alshawwa, Samar Zuhair
Al kamaly, Omkulthom
Kara, Mohammed
Bendaoud, Ahmed
El-Assri, El-Mehdi
Tlemcani, Sara
El Khomsi, Mostafa
Lahkimi, Amal
author_sort Hmamou, Anouar
collection PubMed
description The objective of this study is to valorize Papaver rhoeas L. from the Taounate region of Morocco by determining the total polyphenol content (TPC), the total flavonoid content (TFC) and the antioxidant and antimicrobial activities of four organs. The quantification of TPC and TFC in root, stem, leaf and flower extracts (RE, SE, LE and FE, respectively) was estimated by the Folin–Ciocalteu reaction and the aluminum trichloride method, respectively. Two tests were used to assess antioxidant power: the DPPH test and TAC assay. The antimicrobial activity was studied against five pathogenic bacteria and yeast, using two methods: disk diffusion and microdilution. The TPC in LE and LF was twice as high as that in RE and SE (24.24 and 22.10 mg GAE/g, respectively). The TFC values in the four extracts were very close and varied between 4.50 mg QE/g in the FE and 4.38 mg QE/g in the RE. The LE and FE showed low DPPH values with IC50 = 0.50 and 0.52 mg/mL, respectively. The TAC measurement revealed the presence of a significant amount of antioxidants in the studied extracts, mainly in LE and FE (6.60 and 5.53 mg AAE/g, respectively). The antimicrobial activity results revealed significant activity on almost all of the tested strains. The MIC of FE and SE against E. coli 57 was 1.56 and 0.78 mg/mL, respectively, while against the S. aureus it was 50 and 25 mg/mL, respectively. The low MLC value (1.56 mg/mL) was recorded against E. coli 57 by RE and SE.
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spelling pubmed-88400912022-02-13 Total Phenolic Content and Antioxidant and Antimicrobial Activities of Papaver rhoeas L. Organ Extracts Growing in Taounate Region, Morocco Hmamou, Anouar Eloutassi, Noureddine Alshawwa, Samar Zuhair Al kamaly, Omkulthom Kara, Mohammed Bendaoud, Ahmed El-Assri, El-Mehdi Tlemcani, Sara El Khomsi, Mostafa Lahkimi, Amal Molecules Article The objective of this study is to valorize Papaver rhoeas L. from the Taounate region of Morocco by determining the total polyphenol content (TPC), the total flavonoid content (TFC) and the antioxidant and antimicrobial activities of four organs. The quantification of TPC and TFC in root, stem, leaf and flower extracts (RE, SE, LE and FE, respectively) was estimated by the Folin–Ciocalteu reaction and the aluminum trichloride method, respectively. Two tests were used to assess antioxidant power: the DPPH test and TAC assay. The antimicrobial activity was studied against five pathogenic bacteria and yeast, using two methods: disk diffusion and microdilution. The TPC in LE and LF was twice as high as that in RE and SE (24.24 and 22.10 mg GAE/g, respectively). The TFC values in the four extracts were very close and varied between 4.50 mg QE/g in the FE and 4.38 mg QE/g in the RE. The LE and FE showed low DPPH values with IC50 = 0.50 and 0.52 mg/mL, respectively. The TAC measurement revealed the presence of a significant amount of antioxidants in the studied extracts, mainly in LE and FE (6.60 and 5.53 mg AAE/g, respectively). The antimicrobial activity results revealed significant activity on almost all of the tested strains. The MIC of FE and SE against E. coli 57 was 1.56 and 0.78 mg/mL, respectively, while against the S. aureus it was 50 and 25 mg/mL, respectively. The low MLC value (1.56 mg/mL) was recorded against E. coli 57 by RE and SE. MDPI 2022-01-27 /pmc/articles/PMC8840091/ /pubmed/35164114 http://dx.doi.org/10.3390/molecules27030854 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hmamou, Anouar
Eloutassi, Noureddine
Alshawwa, Samar Zuhair
Al kamaly, Omkulthom
Kara, Mohammed
Bendaoud, Ahmed
El-Assri, El-Mehdi
Tlemcani, Sara
El Khomsi, Mostafa
Lahkimi, Amal
Total Phenolic Content and Antioxidant and Antimicrobial Activities of Papaver rhoeas L. Organ Extracts Growing in Taounate Region, Morocco
title Total Phenolic Content and Antioxidant and Antimicrobial Activities of Papaver rhoeas L. Organ Extracts Growing in Taounate Region, Morocco
title_full Total Phenolic Content and Antioxidant and Antimicrobial Activities of Papaver rhoeas L. Organ Extracts Growing in Taounate Region, Morocco
title_fullStr Total Phenolic Content and Antioxidant and Antimicrobial Activities of Papaver rhoeas L. Organ Extracts Growing in Taounate Region, Morocco
title_full_unstemmed Total Phenolic Content and Antioxidant and Antimicrobial Activities of Papaver rhoeas L. Organ Extracts Growing in Taounate Region, Morocco
title_short Total Phenolic Content and Antioxidant and Antimicrobial Activities of Papaver rhoeas L. Organ Extracts Growing in Taounate Region, Morocco
title_sort total phenolic content and antioxidant and antimicrobial activities of papaver rhoeas l. organ extracts growing in taounate region, morocco
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840091/
https://www.ncbi.nlm.nih.gov/pubmed/35164114
http://dx.doi.org/10.3390/molecules27030854
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