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Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics

The present research investigates the tuber proteome of the ‘medicinal’ plant Jerusalem artichoke (abbreviated as JA) (Helianthus tuberosus L.) using a high-throughput proteomics technique. Although JA has been historically known to the Native Americans, it was introduced to Europe in the late 19th...

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Autores principales: Bakku, Ranjith Kumar, Gupta, Ravi, Min, Cheol-Woo, Kim, Sun-Tae, Takahashi, Genboku, Shibato, Junko, Shioda, Seiji, Takenoya, Fumiko, Agrawal, Ganesh Kumar, Rakwal, Randeep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840128/
https://www.ncbi.nlm.nih.gov/pubmed/35164374
http://dx.doi.org/10.3390/molecules27031111
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author Bakku, Ranjith Kumar
Gupta, Ravi
Min, Cheol-Woo
Kim, Sun-Tae
Takahashi, Genboku
Shibato, Junko
Shioda, Seiji
Takenoya, Fumiko
Agrawal, Ganesh Kumar
Rakwal, Randeep
author_facet Bakku, Ranjith Kumar
Gupta, Ravi
Min, Cheol-Woo
Kim, Sun-Tae
Takahashi, Genboku
Shibato, Junko
Shioda, Seiji
Takenoya, Fumiko
Agrawal, Ganesh Kumar
Rakwal, Randeep
author_sort Bakku, Ranjith Kumar
collection PubMed
description The present research investigates the tuber proteome of the ‘medicinal’ plant Jerusalem artichoke (abbreviated as JA) (Helianthus tuberosus L.) using a high-throughput proteomics technique. Although JA has been historically known to the Native Americans, it was introduced to Europe in the late 19th century and later spread to Japan (referred to as ‘kiku-imo’) as a folk remedy for diabetes. Genboku Takahashi research group has been working on the cultivation and utilization of kiku-imo tuber as a traditional/alternative medicine in daily life and researched on the lowering of blood sugar level, HbA1c, etc., in human subjects (unpublished data). Understanding the protein components of the tuber may shed light on its healing properties, especially related to diabetes. Using three commercially processed JA tuber products (dried powder and dried chips) we performed total protein extraction on the powdered samples using a label-free quantitate proteomic approach (mass spectrometry) and catalogued for the first time a comprehensive protein list for the JA tuber. A total of 2967 protein groups were identified, statistically analyzed, and further categorized into different protein classes using bioinformatics techniques. We discussed the association of these proteins to health and disease regulatory metabolism. Data are available via ProteomeXchange with identifier PXD030744.
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spelling pubmed-88401282022-02-13 Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics Bakku, Ranjith Kumar Gupta, Ravi Min, Cheol-Woo Kim, Sun-Tae Takahashi, Genboku Shibato, Junko Shioda, Seiji Takenoya, Fumiko Agrawal, Ganesh Kumar Rakwal, Randeep Molecules Article The present research investigates the tuber proteome of the ‘medicinal’ plant Jerusalem artichoke (abbreviated as JA) (Helianthus tuberosus L.) using a high-throughput proteomics technique. Although JA has been historically known to the Native Americans, it was introduced to Europe in the late 19th century and later spread to Japan (referred to as ‘kiku-imo’) as a folk remedy for diabetes. Genboku Takahashi research group has been working on the cultivation and utilization of kiku-imo tuber as a traditional/alternative medicine in daily life and researched on the lowering of blood sugar level, HbA1c, etc., in human subjects (unpublished data). Understanding the protein components of the tuber may shed light on its healing properties, especially related to diabetes. Using three commercially processed JA tuber products (dried powder and dried chips) we performed total protein extraction on the powdered samples using a label-free quantitate proteomic approach (mass spectrometry) and catalogued for the first time a comprehensive protein list for the JA tuber. A total of 2967 protein groups were identified, statistically analyzed, and further categorized into different protein classes using bioinformatics techniques. We discussed the association of these proteins to health and disease regulatory metabolism. Data are available via ProteomeXchange with identifier PXD030744. MDPI 2022-02-07 /pmc/articles/PMC8840128/ /pubmed/35164374 http://dx.doi.org/10.3390/molecules27031111 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bakku, Ranjith Kumar
Gupta, Ravi
Min, Cheol-Woo
Kim, Sun-Tae
Takahashi, Genboku
Shibato, Junko
Shioda, Seiji
Takenoya, Fumiko
Agrawal, Ganesh Kumar
Rakwal, Randeep
Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics
title Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics
title_full Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics
title_fullStr Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics
title_full_unstemmed Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics
title_short Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics
title_sort unravelling the helianthus tuberosus l. (jerusalem artichoke, kiku-imo) tuber proteome by label-free quantitative proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840128/
https://www.ncbi.nlm.nih.gov/pubmed/35164374
http://dx.doi.org/10.3390/molecules27031111
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