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The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells

Nine samples of ethanolic extracts of poplar-type propolis (EEP) originated from South-Eastern Poland were analyzed in terms of the diversity of the flora around the apiary. The mineral composition, antioxidant properties, polyphenolic profile (HPTLC), and main polyphenolic constituents (HPLC-DAD) w...

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Autores principales: Miłek, Michał, Ciszkowicz, Ewa, Tomczyk, Monika, Sidor, Ewelina, Zaguła, Grzegorz, Lecka-Szlachta, Katarzyna, Pasternakiewicz, Anna, Dżugan, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840218/
https://www.ncbi.nlm.nih.gov/pubmed/35163989
http://dx.doi.org/10.3390/molecules27030725
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author Miłek, Michał
Ciszkowicz, Ewa
Tomczyk, Monika
Sidor, Ewelina
Zaguła, Grzegorz
Lecka-Szlachta, Katarzyna
Pasternakiewicz, Anna
Dżugan, Małgorzata
author_facet Miłek, Michał
Ciszkowicz, Ewa
Tomczyk, Monika
Sidor, Ewelina
Zaguła, Grzegorz
Lecka-Szlachta, Katarzyna
Pasternakiewicz, Anna
Dżugan, Małgorzata
author_sort Miłek, Michał
collection PubMed
description Nine samples of ethanolic extracts of poplar-type propolis (EEP) originated from South-Eastern Poland were analyzed in terms of the diversity of the flora around the apiary. The mineral composition, antioxidant properties, polyphenolic profile (HPTLC), and main polyphenolic constituents (HPLC-DAD) were determined. Only minor differences in chemical composition and antioxidant capacity between tested EEPs were found regardless of their botanical origin. However, the biological activity of the EEPs was more diversified. The tested EEPs showed stronger antibacterial activity against Gram-negative bacteria (Escherichia coli) compared to Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis). Staphylococci biofilm inhibition occurred as a result of exposure to the action of four out of nine EEPs (P1–P4). Due to the various compositions of individual EEPs, a different MCF-7 cellular response was observed according to inhibition of cells migration and proliferation. Almost every sample inhibited the migration of breast cancer cells at a low concentration (0.04 µg/mL) of propolis. Even at the lowest concentration (0.02 µg/mL), each EEP inhibited the proliferation of MCF-7 cells, however, the level of inhibition varied between samples.
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spelling pubmed-88402182022-02-13 The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells Miłek, Michał Ciszkowicz, Ewa Tomczyk, Monika Sidor, Ewelina Zaguła, Grzegorz Lecka-Szlachta, Katarzyna Pasternakiewicz, Anna Dżugan, Małgorzata Molecules Article Nine samples of ethanolic extracts of poplar-type propolis (EEP) originated from South-Eastern Poland were analyzed in terms of the diversity of the flora around the apiary. The mineral composition, antioxidant properties, polyphenolic profile (HPTLC), and main polyphenolic constituents (HPLC-DAD) were determined. Only minor differences in chemical composition and antioxidant capacity between tested EEPs were found regardless of their botanical origin. However, the biological activity of the EEPs was more diversified. The tested EEPs showed stronger antibacterial activity against Gram-negative bacteria (Escherichia coli) compared to Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis). Staphylococci biofilm inhibition occurred as a result of exposure to the action of four out of nine EEPs (P1–P4). Due to the various compositions of individual EEPs, a different MCF-7 cellular response was observed according to inhibition of cells migration and proliferation. Almost every sample inhibited the migration of breast cancer cells at a low concentration (0.04 µg/mL) of propolis. Even at the lowest concentration (0.02 µg/mL), each EEP inhibited the proliferation of MCF-7 cells, however, the level of inhibition varied between samples. MDPI 2022-01-22 /pmc/articles/PMC8840218/ /pubmed/35163989 http://dx.doi.org/10.3390/molecules27030725 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Miłek, Michał
Ciszkowicz, Ewa
Tomczyk, Monika
Sidor, Ewelina
Zaguła, Grzegorz
Lecka-Szlachta, Katarzyna
Pasternakiewicz, Anna
Dżugan, Małgorzata
The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells
title The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells
title_full The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells
title_fullStr The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells
title_full_unstemmed The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells
title_short The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells
title_sort study of chemical profile and antioxidant properties of poplar-type polish propolis considering local flora diversity in relation to antibacterial and anticancer activities in human breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840218/
https://www.ncbi.nlm.nih.gov/pubmed/35163989
http://dx.doi.org/10.3390/molecules27030725
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