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The Pathways to Create Containers for Bacteriophage Delivery
Antimicrobial resistance is a global public health threat. One of the possible ways to solve this problem is phage therapy, but the instability of bacteriophages hinders the development of this approach. A bacteriophage delivery system that stabilizes the phage is one of the possible solutions to th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840248/ https://www.ncbi.nlm.nih.gov/pubmed/35160602 http://dx.doi.org/10.3390/polym14030613 |
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author | Musin, Egor V. Kim, Aleksandr L. Dubrovskii, Alexey V. Ariskina, Elena V. Kudryashova, Ekaterina B. Tikhonenko, Sergey A. |
author_facet | Musin, Egor V. Kim, Aleksandr L. Dubrovskii, Alexey V. Ariskina, Elena V. Kudryashova, Ekaterina B. Tikhonenko, Sergey A. |
author_sort | Musin, Egor V. |
collection | PubMed |
description | Antimicrobial resistance is a global public health threat. One of the possible ways to solve this problem is phage therapy, but the instability of bacteriophages hinders the development of this approach. A bacteriophage delivery system that stabilizes the phage is one of the possible solutions to this problem. This study is dedicated to exploring methods to create encapsulated forms of bacteriophages for delivery. We studied the effect of proteolytic enzymes on the destruction of the polyelectrolyte microcapsule shell and revealed that protease from Streptomyces griseus was able to destroy the membrane of the microcapsule (dextran sulfate/polyarginine)(3) ((DS/PArg)(3)). In addition, the protease decreased the activity of the bacteriophage in the second hour of incubation, and the phage lost activity after 16 h. It was found that a medium with pH 9.02 did not affect the survival of the bacteriophage or E. coli. The bacteriophages were encapsulated into polyelectrolyte microcapsules (DS/PArg)(3). It was established that it is impossible to use microcapsules as a means of delivering bacteriophages since the bacteriophages are inactivated. When bacteriophages were included inside a CaCO(3) core, it was demonstrated that the phage retained activity before and after the dissolution of the CaCO(3) particle. From the results of this study, we recommend using CaCO(3) microparticles as a container for bacteriophage delivery through the acidic stomach barrier. |
format | Online Article Text |
id | pubmed-8840248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88402482022-02-13 The Pathways to Create Containers for Bacteriophage Delivery Musin, Egor V. Kim, Aleksandr L. Dubrovskii, Alexey V. Ariskina, Elena V. Kudryashova, Ekaterina B. Tikhonenko, Sergey A. Polymers (Basel) Article Antimicrobial resistance is a global public health threat. One of the possible ways to solve this problem is phage therapy, but the instability of bacteriophages hinders the development of this approach. A bacteriophage delivery system that stabilizes the phage is one of the possible solutions to this problem. This study is dedicated to exploring methods to create encapsulated forms of bacteriophages for delivery. We studied the effect of proteolytic enzymes on the destruction of the polyelectrolyte microcapsule shell and revealed that protease from Streptomyces griseus was able to destroy the membrane of the microcapsule (dextran sulfate/polyarginine)(3) ((DS/PArg)(3)). In addition, the protease decreased the activity of the bacteriophage in the second hour of incubation, and the phage lost activity after 16 h. It was found that a medium with pH 9.02 did not affect the survival of the bacteriophage or E. coli. The bacteriophages were encapsulated into polyelectrolyte microcapsules (DS/PArg)(3). It was established that it is impossible to use microcapsules as a means of delivering bacteriophages since the bacteriophages are inactivated. When bacteriophages were included inside a CaCO(3) core, it was demonstrated that the phage retained activity before and after the dissolution of the CaCO(3) particle. From the results of this study, we recommend using CaCO(3) microparticles as a container for bacteriophage delivery through the acidic stomach barrier. MDPI 2022-02-04 /pmc/articles/PMC8840248/ /pubmed/35160602 http://dx.doi.org/10.3390/polym14030613 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Musin, Egor V. Kim, Aleksandr L. Dubrovskii, Alexey V. Ariskina, Elena V. Kudryashova, Ekaterina B. Tikhonenko, Sergey A. The Pathways to Create Containers for Bacteriophage Delivery |
title | The Pathways to Create Containers for Bacteriophage Delivery |
title_full | The Pathways to Create Containers for Bacteriophage Delivery |
title_fullStr | The Pathways to Create Containers for Bacteriophage Delivery |
title_full_unstemmed | The Pathways to Create Containers for Bacteriophage Delivery |
title_short | The Pathways to Create Containers for Bacteriophage Delivery |
title_sort | pathways to create containers for bacteriophage delivery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840248/ https://www.ncbi.nlm.nih.gov/pubmed/35160602 http://dx.doi.org/10.3390/polym14030613 |
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