Cargando…

Anti-Osteoporosis Effect of Perilla frutescens Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation

Osteoporosis is the result of an imbalance in the bone-remodeling process via an increase in osteoclastic activity and a decrease in osteoblastic activity. Our previous studies have shown that Perilla frutescens seed meal has anti-osteoclastogenic activity. However, the role of perilla leaf hexane f...

Descripción completa

Detalles Bibliográficos
Autores principales: Phromnoi, Kanokkarn, Yodkeeree, Supachai, Pintha, Komsak, Mapoung, Sariya, Suttajit, Maitree, Saenjum, Chalermpong, Dejkriengkraikul, Pornngarm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840259/
https://www.ncbi.nlm.nih.gov/pubmed/35164085
http://dx.doi.org/10.3390/molecules27030824
_version_ 1784650576122871808
author Phromnoi, Kanokkarn
Yodkeeree, Supachai
Pintha, Komsak
Mapoung, Sariya
Suttajit, Maitree
Saenjum, Chalermpong
Dejkriengkraikul, Pornngarm
author_facet Phromnoi, Kanokkarn
Yodkeeree, Supachai
Pintha, Komsak
Mapoung, Sariya
Suttajit, Maitree
Saenjum, Chalermpong
Dejkriengkraikul, Pornngarm
author_sort Phromnoi, Kanokkarn
collection PubMed
description Osteoporosis is the result of an imbalance in the bone-remodeling process via an increase in osteoclastic activity and a decrease in osteoblastic activity. Our previous studies have shown that Perilla frutescens seed meal has anti-osteoclastogenic activity. However, the role of perilla leaf hexane fraction (PLH) in osteoporosis has not yet been investigated and reported. In this study, we aimed to investigate the effects of PLH in osteoclast differentiation and osteogenic potential using cell-based experiments in vitro. From HPLC analysis, we found that PLH contained high luteolin and baicalein. PLH was shown to inhibit RANKL-induced ROS production and tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts. Moreover, PLH significantly downregulated the RANKL-induced MAPK and NF-κB signaling pathways, leading to the attenuation of NFATc1 and MMP-9 expression. In contrast, PLH enhanced osteoblast function by regulating alkaline phosphatase (ALP) and restoring TNF-α-suppressed osteoblast proliferation and osteogenic potential. Thus, luteolin and baicalein-rich PLH inhibits osteoclast differentiation but promotes the function of osteoblasts. Collectively, our data provide new evidence that suggests that PLH may be a valuable anti-osteoporosis agent.
format Online
Article
Text
id pubmed-8840259
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88402592022-02-13 Anti-Osteoporosis Effect of Perilla frutescens Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation Phromnoi, Kanokkarn Yodkeeree, Supachai Pintha, Komsak Mapoung, Sariya Suttajit, Maitree Saenjum, Chalermpong Dejkriengkraikul, Pornngarm Molecules Article Osteoporosis is the result of an imbalance in the bone-remodeling process via an increase in osteoclastic activity and a decrease in osteoblastic activity. Our previous studies have shown that Perilla frutescens seed meal has anti-osteoclastogenic activity. However, the role of perilla leaf hexane fraction (PLH) in osteoporosis has not yet been investigated and reported. In this study, we aimed to investigate the effects of PLH in osteoclast differentiation and osteogenic potential using cell-based experiments in vitro. From HPLC analysis, we found that PLH contained high luteolin and baicalein. PLH was shown to inhibit RANKL-induced ROS production and tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts. Moreover, PLH significantly downregulated the RANKL-induced MAPK and NF-κB signaling pathways, leading to the attenuation of NFATc1 and MMP-9 expression. In contrast, PLH enhanced osteoblast function by regulating alkaline phosphatase (ALP) and restoring TNF-α-suppressed osteoblast proliferation and osteogenic potential. Thus, luteolin and baicalein-rich PLH inhibits osteoclast differentiation but promotes the function of osteoblasts. Collectively, our data provide new evidence that suggests that PLH may be a valuable anti-osteoporosis agent. MDPI 2022-01-26 /pmc/articles/PMC8840259/ /pubmed/35164085 http://dx.doi.org/10.3390/molecules27030824 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Phromnoi, Kanokkarn
Yodkeeree, Supachai
Pintha, Komsak
Mapoung, Sariya
Suttajit, Maitree
Saenjum, Chalermpong
Dejkriengkraikul, Pornngarm
Anti-Osteoporosis Effect of Perilla frutescens Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation
title Anti-Osteoporosis Effect of Perilla frutescens Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation
title_full Anti-Osteoporosis Effect of Perilla frutescens Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation
title_fullStr Anti-Osteoporosis Effect of Perilla frutescens Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation
title_full_unstemmed Anti-Osteoporosis Effect of Perilla frutescens Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation
title_short Anti-Osteoporosis Effect of Perilla frutescens Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation
title_sort anti-osteoporosis effect of perilla frutescens leaf hexane fraction through regulating osteoclast and osteoblast differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840259/
https://www.ncbi.nlm.nih.gov/pubmed/35164085
http://dx.doi.org/10.3390/molecules27030824
work_keys_str_mv AT phromnoikanokkarn antiosteoporosiseffectofperillafrutescensleafhexanefractionthroughregulatingosteoclastandosteoblastdifferentiation
AT yodkeereesupachai antiosteoporosiseffectofperillafrutescensleafhexanefractionthroughregulatingosteoclastandosteoblastdifferentiation
AT pinthakomsak antiosteoporosiseffectofperillafrutescensleafhexanefractionthroughregulatingosteoclastandosteoblastdifferentiation
AT mapoungsariya antiosteoporosiseffectofperillafrutescensleafhexanefractionthroughregulatingosteoclastandosteoblastdifferentiation
AT suttajitmaitree antiosteoporosiseffectofperillafrutescensleafhexanefractionthroughregulatingosteoclastandosteoblastdifferentiation
AT saenjumchalermpong antiosteoporosiseffectofperillafrutescensleafhexanefractionthroughregulatingosteoclastandosteoblastdifferentiation
AT dejkriengkraikulpornngarm antiosteoporosiseffectofperillafrutescensleafhexanefractionthroughregulatingosteoclastandosteoblastdifferentiation