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Estimation of the Local Concentration of the Markedly Dense Hydroxyl Radical Generation Induced by X-rays in Water
The linear-density (number of molecules on an arbitrary distance) of X-ray-induced markedly dense hydroxyl radicals (•OH) in water was estimated based on EPR spin-trapping measurement. A lower (0.13 mM–2.3 M) concentration series of DMPO water solutions and higher (1.7–6.0 M) concentration series of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840408/ https://www.ncbi.nlm.nih.gov/pubmed/35163857 http://dx.doi.org/10.3390/molecules27030592 |
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author | Matsumoto, Ken-ichiro Ueno, Megumi Shoji, Yoshimi Nakanishi, Ikuo |
author_facet | Matsumoto, Ken-ichiro Ueno, Megumi Shoji, Yoshimi Nakanishi, Ikuo |
author_sort | Matsumoto, Ken-ichiro |
collection | PubMed |
description | The linear-density (number of molecules on an arbitrary distance) of X-ray-induced markedly dense hydroxyl radicals (•OH) in water was estimated based on EPR spin-trapping measurement. A lower (0.13 mM–2.3 M) concentration series of DMPO water solutions and higher (1.7–6.0 M) concentration series of DMPO water solutions plus neat DMPO liquid (8.8 M as DMPO) were irradiated with 32 Gy of X-rays. Then, the yield of DMPO-OH in DMPO water solutions and the total spin-adduct of DMPO in neat DMPO were quantified. For the higher concentration DMPO series, the EPR peak area was estimated by double integration, and the baseline correction of the integral spectrum is necessary for accurate estimation of the peak area. The preparation of a suitable standard sample corresponding to the electric permittivity according to DMPO concentration was quite important for quantification of DMPO-OH, especially in DMPO concentration beyond 2 M. The linear-density of •OH generation in water by X-ray irradiation was estimated from the inflection point on the plot of the DMPO-OH yield versus DMPO linear-density. The linear-density of X-ray-induced markedly dense •OH was estimated as 1168 μm(−1), which was converted to 0.86 nm as the intermolecular distance and 2.6 M as the local concentration. |
format | Online Article Text |
id | pubmed-8840408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88404082022-02-13 Estimation of the Local Concentration of the Markedly Dense Hydroxyl Radical Generation Induced by X-rays in Water Matsumoto, Ken-ichiro Ueno, Megumi Shoji, Yoshimi Nakanishi, Ikuo Molecules Article The linear-density (number of molecules on an arbitrary distance) of X-ray-induced markedly dense hydroxyl radicals (•OH) in water was estimated based on EPR spin-trapping measurement. A lower (0.13 mM–2.3 M) concentration series of DMPO water solutions and higher (1.7–6.0 M) concentration series of DMPO water solutions plus neat DMPO liquid (8.8 M as DMPO) were irradiated with 32 Gy of X-rays. Then, the yield of DMPO-OH in DMPO water solutions and the total spin-adduct of DMPO in neat DMPO were quantified. For the higher concentration DMPO series, the EPR peak area was estimated by double integration, and the baseline correction of the integral spectrum is necessary for accurate estimation of the peak area. The preparation of a suitable standard sample corresponding to the electric permittivity according to DMPO concentration was quite important for quantification of DMPO-OH, especially in DMPO concentration beyond 2 M. The linear-density of •OH generation in water by X-ray irradiation was estimated from the inflection point on the plot of the DMPO-OH yield versus DMPO linear-density. The linear-density of X-ray-induced markedly dense •OH was estimated as 1168 μm(−1), which was converted to 0.86 nm as the intermolecular distance and 2.6 M as the local concentration. MDPI 2022-01-18 /pmc/articles/PMC8840408/ /pubmed/35163857 http://dx.doi.org/10.3390/molecules27030592 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Matsumoto, Ken-ichiro Ueno, Megumi Shoji, Yoshimi Nakanishi, Ikuo Estimation of the Local Concentration of the Markedly Dense Hydroxyl Radical Generation Induced by X-rays in Water |
title | Estimation of the Local Concentration of the Markedly Dense Hydroxyl Radical Generation Induced by X-rays in Water |
title_full | Estimation of the Local Concentration of the Markedly Dense Hydroxyl Radical Generation Induced by X-rays in Water |
title_fullStr | Estimation of the Local Concentration of the Markedly Dense Hydroxyl Radical Generation Induced by X-rays in Water |
title_full_unstemmed | Estimation of the Local Concentration of the Markedly Dense Hydroxyl Radical Generation Induced by X-rays in Water |
title_short | Estimation of the Local Concentration of the Markedly Dense Hydroxyl Radical Generation Induced by X-rays in Water |
title_sort | estimation of the local concentration of the markedly dense hydroxyl radical generation induced by x-rays in water |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840408/ https://www.ncbi.nlm.nih.gov/pubmed/35163857 http://dx.doi.org/10.3390/molecules27030592 |
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