Cargando…

LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan

This study aimed to evaluate the antioxidant activity and total phenolic content (TPC) and total flavonoid content (TFC) of crude extracts obtained from three Asclepiadaceae species, namely, Calotropis procera L., Peruglaria tomentosa L., and Pentatropis spiralis (Forsk.) Decne. Both butanol and aq....

Descripción completa

Detalles Bibliográficos
Autores principales: Al-Dalahmeh, Yousef, Al-Bataineh, Nezar, Al-Balawi, Sara S., Lahham, Jamil N., Al-Momani, Idrees F., Al-Sheraideh, Mohammed S., Mayyas, Abdulraouf S., Abu Orabi, Sultan T., Al-Qudah, Mahmoud A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840462/
https://www.ncbi.nlm.nih.gov/pubmed/35164120
http://dx.doi.org/10.3390/molecules27030859
_version_ 1784650625495072768
author Al-Dalahmeh, Yousef
Al-Bataineh, Nezar
Al-Balawi, Sara S.
Lahham, Jamil N.
Al-Momani, Idrees F.
Al-Sheraideh, Mohammed S.
Mayyas, Abdulraouf S.
Abu Orabi, Sultan T.
Al-Qudah, Mahmoud A.
author_facet Al-Dalahmeh, Yousef
Al-Bataineh, Nezar
Al-Balawi, Sara S.
Lahham, Jamil N.
Al-Momani, Idrees F.
Al-Sheraideh, Mohammed S.
Mayyas, Abdulraouf S.
Abu Orabi, Sultan T.
Al-Qudah, Mahmoud A.
author_sort Al-Dalahmeh, Yousef
collection PubMed
description This study aimed to evaluate the antioxidant activity and total phenolic content (TPC) and total flavonoid content (TFC) of crude extracts obtained from three Asclepiadaceae species, namely, Calotropis procera L., Peruglaria tomentosa L., and Pentatropis spiralis (Forsk.) Decne. Both butanol and aq. methanol extracts of the three species showed the highest amount of phenol and flavonoid contents, which exhibited the greatest antioxidant activity in the scavenging of 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS), ferrous chelating effect (FIC), and hydroxyl radical (HDR) assays. Phytochemical screening of the extracts revealed the presence of alkaloids, tannins, sponins, flavonoids, terpenoids, and glycosides. LC-MS analysis was carried out to identify the major compounds from each crude extract. A total of 12 phenolic compounds in the extracts of the 3 species were identified and quantified, including 9 flavonoids, 2 hydroxybenzoic acids, and 3 hydroxycinnamic acids. The current study also revealed a good correlation between total phenolic contents and the observed antioxidant activity of the crude extracts.
format Online
Article
Text
id pubmed-8840462
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88404622022-02-13 LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan Al-Dalahmeh, Yousef Al-Bataineh, Nezar Al-Balawi, Sara S. Lahham, Jamil N. Al-Momani, Idrees F. Al-Sheraideh, Mohammed S. Mayyas, Abdulraouf S. Abu Orabi, Sultan T. Al-Qudah, Mahmoud A. Molecules Article This study aimed to evaluate the antioxidant activity and total phenolic content (TPC) and total flavonoid content (TFC) of crude extracts obtained from three Asclepiadaceae species, namely, Calotropis procera L., Peruglaria tomentosa L., and Pentatropis spiralis (Forsk.) Decne. Both butanol and aq. methanol extracts of the three species showed the highest amount of phenol and flavonoid contents, which exhibited the greatest antioxidant activity in the scavenging of 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS), ferrous chelating effect (FIC), and hydroxyl radical (HDR) assays. Phytochemical screening of the extracts revealed the presence of alkaloids, tannins, sponins, flavonoids, terpenoids, and glycosides. LC-MS analysis was carried out to identify the major compounds from each crude extract. A total of 12 phenolic compounds in the extracts of the 3 species were identified and quantified, including 9 flavonoids, 2 hydroxybenzoic acids, and 3 hydroxycinnamic acids. The current study also revealed a good correlation between total phenolic contents and the observed antioxidant activity of the crude extracts. MDPI 2022-01-27 /pmc/articles/PMC8840462/ /pubmed/35164120 http://dx.doi.org/10.3390/molecules27030859 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Dalahmeh, Yousef
Al-Bataineh, Nezar
Al-Balawi, Sara S.
Lahham, Jamil N.
Al-Momani, Idrees F.
Al-Sheraideh, Mohammed S.
Mayyas, Abdulraouf S.
Abu Orabi, Sultan T.
Al-Qudah, Mahmoud A.
LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan
title LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan
title_full LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan
title_fullStr LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan
title_full_unstemmed LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan
title_short LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan
title_sort lc-ms/ms screening, total phenolic, flavonoid and antioxidant contents of crude extracts from three asclepiadaceae species growing in jordan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840462/
https://www.ncbi.nlm.nih.gov/pubmed/35164120
http://dx.doi.org/10.3390/molecules27030859
work_keys_str_mv AT aldalahmehyousef lcmsmsscreeningtotalphenolicflavonoidandantioxidantcontentsofcrudeextractsfromthreeasclepiadaceaespeciesgrowinginjordan
AT albatainehnezar lcmsmsscreeningtotalphenolicflavonoidandantioxidantcontentsofcrudeextractsfromthreeasclepiadaceaespeciesgrowinginjordan
AT albalawisaras lcmsmsscreeningtotalphenolicflavonoidandantioxidantcontentsofcrudeextractsfromthreeasclepiadaceaespeciesgrowinginjordan
AT lahhamjamiln lcmsmsscreeningtotalphenolicflavonoidandantioxidantcontentsofcrudeextractsfromthreeasclepiadaceaespeciesgrowinginjordan
AT almomaniidreesf lcmsmsscreeningtotalphenolicflavonoidandantioxidantcontentsofcrudeextractsfromthreeasclepiadaceaespeciesgrowinginjordan
AT alsheraidehmohammeds lcmsmsscreeningtotalphenolicflavonoidandantioxidantcontentsofcrudeextractsfromthreeasclepiadaceaespeciesgrowinginjordan
AT mayyasabdulraoufs lcmsmsscreeningtotalphenolicflavonoidandantioxidantcontentsofcrudeextractsfromthreeasclepiadaceaespeciesgrowinginjordan
AT abuorabisultant lcmsmsscreeningtotalphenolicflavonoidandantioxidantcontentsofcrudeextractsfromthreeasclepiadaceaespeciesgrowinginjordan
AT alqudahmahmouda lcmsmsscreeningtotalphenolicflavonoidandantioxidantcontentsofcrudeextractsfromthreeasclepiadaceaespeciesgrowinginjordan