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DNA Barcoding Study of Representative Thymus Species in Bulgaria

We present a study on the taxonomy of eleven Thymus species, belonging to two sections and occurring naturally in Bulgaria. Four DNA barcoding markers—matK, rbcL, trnH-psbA and ITS—were applied to discriminate the species and to reveal their phylogenetic relationships. The results showed that rbcL h...

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Detalles Bibliográficos
Autores principales: Aneva, Ina, Zhelev, Petar, Bonchev, Georgi, Boycheva, Irina, Simeonova, Stiliana, Kancheva, Denitsa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840554/
https://www.ncbi.nlm.nih.gov/pubmed/35161250
http://dx.doi.org/10.3390/plants11030270
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author Aneva, Ina
Zhelev, Petar
Bonchev, Georgi
Boycheva, Irina
Simeonova, Stiliana
Kancheva, Denitsa
author_facet Aneva, Ina
Zhelev, Petar
Bonchev, Georgi
Boycheva, Irina
Simeonova, Stiliana
Kancheva, Denitsa
author_sort Aneva, Ina
collection PubMed
description We present a study on the taxonomy of eleven Thymus species, belonging to two sections and occurring naturally in Bulgaria. Four DNA barcoding markers—matK, rbcL, trnH-psbA and ITS—were applied to discriminate the species and to reveal their phylogenetic relationships. The results showed that rbcL has the lowest discriminating power regarding the studied species, while the other markers yielded results fitting better to the existing taxonomic schemes based on morphological traits. However, even in the case of better performing markers, the results were not straightforward—morphologically distinct species belonging to different sections were grouped together, and closely related species appeared genetically distinct. The results are typical for taxonomically complex groups, such as the genus Thymus, characterized in Bulgaria with great diversity, high percentage of endemism and still requiring a full and comprehensive taxonomic study. The results are discussed in the light of unresolved taxonomic problems and application of DNA barcoding methods.
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spelling pubmed-88405542022-02-13 DNA Barcoding Study of Representative Thymus Species in Bulgaria Aneva, Ina Zhelev, Petar Bonchev, Georgi Boycheva, Irina Simeonova, Stiliana Kancheva, Denitsa Plants (Basel) Article We present a study on the taxonomy of eleven Thymus species, belonging to two sections and occurring naturally in Bulgaria. Four DNA barcoding markers—matK, rbcL, trnH-psbA and ITS—were applied to discriminate the species and to reveal their phylogenetic relationships. The results showed that rbcL has the lowest discriminating power regarding the studied species, while the other markers yielded results fitting better to the existing taxonomic schemes based on morphological traits. However, even in the case of better performing markers, the results were not straightforward—morphologically distinct species belonging to different sections were grouped together, and closely related species appeared genetically distinct. The results are typical for taxonomically complex groups, such as the genus Thymus, characterized in Bulgaria with great diversity, high percentage of endemism and still requiring a full and comprehensive taxonomic study. The results are discussed in the light of unresolved taxonomic problems and application of DNA barcoding methods. MDPI 2022-01-20 /pmc/articles/PMC8840554/ /pubmed/35161250 http://dx.doi.org/10.3390/plants11030270 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aneva, Ina
Zhelev, Petar
Bonchev, Georgi
Boycheva, Irina
Simeonova, Stiliana
Kancheva, Denitsa
DNA Barcoding Study of Representative Thymus Species in Bulgaria
title DNA Barcoding Study of Representative Thymus Species in Bulgaria
title_full DNA Barcoding Study of Representative Thymus Species in Bulgaria
title_fullStr DNA Barcoding Study of Representative Thymus Species in Bulgaria
title_full_unstemmed DNA Barcoding Study of Representative Thymus Species in Bulgaria
title_short DNA Barcoding Study of Representative Thymus Species in Bulgaria
title_sort dna barcoding study of representative thymus species in bulgaria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840554/
https://www.ncbi.nlm.nih.gov/pubmed/35161250
http://dx.doi.org/10.3390/plants11030270
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