Development of the PARMS Marker of the Dominant Genic Male Sterility (DGMS) Line and Its Utilization in Rapeseed (Brassica napus L.) Breeding

The 8029AB line is a dominant genic male sterility (DGMS) two-type line in Brassica napus L., which can be used in a three-line approach for the seed production of rapeseed hybrids. Genetic analyses have demonstrated that the sterility of 8029A is controlled by a single dominant nuclear gene (BnMS5(e)) interacting with one recessive gene (BnMS5(c)). Six pairs of penta-primer amplification refractory mutation system (PARMS) markers were designed according to the sequence of BnMS5(a), BnMS5(c) and BnMS5(e). Two pairs of these PARMS markers were successfully identified and validated. The PARMS markers MS5-1Fc/MS5-1Ft/MS5-1R12 could distinguish BnMS5(c) from BnMS5(a)/BnMS5(e), and the PARMS markers MS5-2Ft/MS5-2Fa/MS5-1R12 could genotype BnMS5(a) and BnMS5(c)/BnMS5(e). The combination of these two pairs of PARMS markers could be used to identify the presence or absence of BnMS5(a)/BnMS5(c)/BnMS5(e) effectively. Consequently, marker-assisted selection can be carried out in the early generation to shorten the breeding period and improve the breeding efficiency.