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Phytochemical analysis and anti-microbial activities of Artemisia spp. and rapid isolation methods of artemisinin
Artemisia species are important traditional medicinal plants of Ethiopia which are used for the treatment of infection and non-infection health problems. The genus Artemisia (Astraceae) consists of about 500 species worldwide. In this study, the main aim was to investigate chemical components of Art...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840944/ https://www.ncbi.nlm.nih.gov/pubmed/35150378 http://dx.doi.org/10.1186/s13568-022-01346-5 |
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author | Mohammed, Seid Dekabo, Aman Hailu, Tilahun |
author_facet | Mohammed, Seid Dekabo, Aman Hailu, Tilahun |
author_sort | Mohammed, Seid |
collection | PubMed |
description | Artemisia species are important traditional medicinal plants of Ethiopia which are used for the treatment of infection and non-infection health problems. The genus Artemisia (Astraceae) consists of about 500 species worldwide. In this study, the main aim was to investigate chemical components of Artemisia spp. (A. abyssinica, A. absinthium and A. annua) and evaluate their antimicrobial activities against bacterial strains. The results indicated that the crude extract of these plants was effective against selected bacterial strains. Here we isolated the well-known antimalarial drug artemisinin (7 mg, 0.004%) from Artemisia annua leaves using a rapid n-hexane fractionation method. The n-hexane extract of A. abyssinica, ethyl acetate extract of A. absinthium and n-hexane of A. annua showed varying degrees of inhibiting effect against bacterial strains such as Staphylococcus aureus ATCC 25923( T), Salmonella enteritidis ATCC13076(T), Klebsiella pneumoniae ATCC1053(T), boydii ATCC1233(T), Escherichia coli ATCC 25922( T), hospital acquired Acinetobacter baumannii. The ethyl acetate extract of Artemisia absinthium (A.ab(e)) showed the maximum inhibiting effect (35 mm) against A. baumannii. The minimum zone of inhibition (< 3 mm) was recorded for test extract of A.ap against Klebsiella pneumoniae ATCC1053(T). Ethyl acetate extract of A, absinthium (A.abe) was more effective against these selected bacterial strains and the zone of inhibition ranged from 5 to 35 mm. The minimum inhibition zone (8 mm) was detected against S. typhimurium ATCC 13311( T) for both A.ac and n-hexane- EtOAc fraction (8:2) of A, abyssinica. The maximum zone of inhibition (25 mm) for fraction (A.ach F4) of A, abyssinica obtained by column chromatography was recorded against S. pyogen ATCC 19,615. However, there was no zone of inhibition detected for boydii ATCC1233(T) due to these test extracts. Significant variations (P ≥ 0.05) were observed between all test extracts of these medicinal plants at 95% of confidence intervals. In this study, clear zones of inhibition were detected for the positive control (standard impregnated disks). Based on our results we recommend that various species of Artemisia spp. seem to have the potential for in-depth investigation for various antimicrobial activities that assisting the effort in searching for antimicrobial lead compounds. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-022-01346-5. |
format | Online Article Text |
id | pubmed-8840944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-88409442022-02-23 Phytochemical analysis and anti-microbial activities of Artemisia spp. and rapid isolation methods of artemisinin Mohammed, Seid Dekabo, Aman Hailu, Tilahun AMB Express Original Article Artemisia species are important traditional medicinal plants of Ethiopia which are used for the treatment of infection and non-infection health problems. The genus Artemisia (Astraceae) consists of about 500 species worldwide. In this study, the main aim was to investigate chemical components of Artemisia spp. (A. abyssinica, A. absinthium and A. annua) and evaluate their antimicrobial activities against bacterial strains. The results indicated that the crude extract of these plants was effective against selected bacterial strains. Here we isolated the well-known antimalarial drug artemisinin (7 mg, 0.004%) from Artemisia annua leaves using a rapid n-hexane fractionation method. The n-hexane extract of A. abyssinica, ethyl acetate extract of A. absinthium and n-hexane of A. annua showed varying degrees of inhibiting effect against bacterial strains such as Staphylococcus aureus ATCC 25923( T), Salmonella enteritidis ATCC13076(T), Klebsiella pneumoniae ATCC1053(T), boydii ATCC1233(T), Escherichia coli ATCC 25922( T), hospital acquired Acinetobacter baumannii. The ethyl acetate extract of Artemisia absinthium (A.ab(e)) showed the maximum inhibiting effect (35 mm) against A. baumannii. The minimum zone of inhibition (< 3 mm) was recorded for test extract of A.ap against Klebsiella pneumoniae ATCC1053(T). Ethyl acetate extract of A, absinthium (A.abe) was more effective against these selected bacterial strains and the zone of inhibition ranged from 5 to 35 mm. The minimum inhibition zone (8 mm) was detected against S. typhimurium ATCC 13311( T) for both A.ac and n-hexane- EtOAc fraction (8:2) of A, abyssinica. The maximum zone of inhibition (25 mm) for fraction (A.ach F4) of A, abyssinica obtained by column chromatography was recorded against S. pyogen ATCC 19,615. However, there was no zone of inhibition detected for boydii ATCC1233(T) due to these test extracts. Significant variations (P ≥ 0.05) were observed between all test extracts of these medicinal plants at 95% of confidence intervals. In this study, clear zones of inhibition were detected for the positive control (standard impregnated disks). Based on our results we recommend that various species of Artemisia spp. seem to have the potential for in-depth investigation for various antimicrobial activities that assisting the effort in searching for antimicrobial lead compounds. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-022-01346-5. Springer Berlin Heidelberg 2022-02-12 /pmc/articles/PMC8840944/ /pubmed/35150378 http://dx.doi.org/10.1186/s13568-022-01346-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Mohammed, Seid Dekabo, Aman Hailu, Tilahun Phytochemical analysis and anti-microbial activities of Artemisia spp. and rapid isolation methods of artemisinin |
title | Phytochemical analysis and anti-microbial activities of Artemisia spp. and rapid isolation methods of artemisinin |
title_full | Phytochemical analysis and anti-microbial activities of Artemisia spp. and rapid isolation methods of artemisinin |
title_fullStr | Phytochemical analysis and anti-microbial activities of Artemisia spp. and rapid isolation methods of artemisinin |
title_full_unstemmed | Phytochemical analysis and anti-microbial activities of Artemisia spp. and rapid isolation methods of artemisinin |
title_short | Phytochemical analysis and anti-microbial activities of Artemisia spp. and rapid isolation methods of artemisinin |
title_sort | phytochemical analysis and anti-microbial activities of artemisia spp. and rapid isolation methods of artemisinin |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8840944/ https://www.ncbi.nlm.nih.gov/pubmed/35150378 http://dx.doi.org/10.1186/s13568-022-01346-5 |
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