Cargando…

Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells

Reliable diagnosis is critical to identify infections of SARS-CoV-2 as well as to evaluate the immune response to virus and vaccines. Consequently, it becomes crucial the isolation of sensitive antibodies to use as immunocapture elements of diagnostic tools. The final bottleneck to achieve these res...

Descripción completa

Detalles Bibliográficos
Autores principales:	De March, Matteo, Terdoslavich, Michela, Polez, Sulena, Guarnaccia, Corrado, Poggianella, Monica, Marcello, Alessandro, Skoko, Nataša, de Marco, Ario
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Inc. 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841003/ https://www.ncbi.nlm.nih.gov/pubmed/35172194 http://dx.doi.org/10.1016/j.pep.2022.106071

Ejemplares similares

Exploring Parametric and Mechanistic Differences between Expi293F(TM) and ExpiCHO-S(TM) Cells for Transient Antibody Production Optimization
por: Zhou, Jing, et al.
Publicado: (2023)

A Simplified and Efficient Process for Insulin Production in Pichia pastoris
por: Polez, Sulena, et al.
Publicado: (2016)

Inhibitors of Protein Glycosylation Are Active against the Coronavirus Severe Acute Respiratory Syndrome Coronavirus SARS-CoV-2
por: Rajasekharan, Sreejith, et al.
Publicado: (2021)

Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin
por: Gurramkonda, Chandrasekhar, et al.
Publicado: (2010)

High Throughput miRNA Screening Identifies miR-574-3p Hyperproductive Effect in CHO Cells
por: Švab, Živa, et al.
Publicado: (2021)

Efficient and inexpensive transient expression of multispecific multivalent antibodies in Expi293 cells
por: Fang, Xiaotian T., et al.
Publicado: (2017)

Interface-Based Design of High-Affinity Affibody Ligands for the Purification of RBD from Spike Proteins
por: Song, Siyuan, et al.
Publicado: (2023)

Antibody Light Chains: Key to Increased Monoclonal Antibody Yields in Expi293 Cells?
por: Gong, Siqi, et al.
Publicado: (2022)

Production and purification of TGFb-1 in CHO-Cells
por: Abdulkerim, Estabraq, et al.
Publicado: (2011)

ExpI and PhzI Are Descendants of the Long Lost Cognate Signal Synthase for SdiA
por: Sabag-Daigle, Anice, et al.
Publicado: (2012)

A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cells
por: Siddiqui, Asim Azhar, et al.
Publicado: (2023)

Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein Expressed in Yeast
por: Nagar, Gaurav, et al.
Publicado: (2023)

Nanobodies Selectively Binding to the Idiotype of a Dengue Virus Neutralizing Antibody Do Not Necessarily Mimic the Viral Epitope
por: Poggianella, Monica, et al.
Publicado: (2023)

Plant phenolic volatiles inhibit quorum sensing in pectobacteria and reduce their virulence by potential binding to ExpI and ExpR proteins
por: Joshi, Janak Raj, et al.
Publicado: (2016)

Multiepitope Proteins for the Differential Detection of IgG Antibodies against RBD of the Spike Protein and Non-RBD Regions of SARS-CoV-2
por: Gomes, Larissa R., et al.
Publicado: (2021)

CHO-produced RBD-Fc subunit vaccines with alternative adjuvants generate immune responses against SARS-CoV-2
por: Laotee, Sedthawut, et al.
Publicado: (2023)

Woodchuck Hepatitis Virus Post-Transcriptional Regulation Element (WPRE) Promotes Anti-CD19 BiTE Expression in Expi293 Cells
por: Moazzami, Reza, et al.
Publicado: (2021)

A step ahead: combining protein purification and correct folding selection
por: de Marco, Ario
Publicado: (2004)

Novel human neutralizing mAbs specific for Spike-RBD of SARS-CoV-2
por: Passariello, Margherita, et al.
Publicado: (2021)

Expression, purification, and study on the efficiency of a new potent recombinant scFv antibody against the SARS-CoV-2 spike RBD in E. coli BL21
por: Yaghoobizadeh, Fatemeh, et al.
Publicado: (2023)

Assessment of mutations on RBD in the Spike protein of SARS-CoV-2 Alpha, Delta and Omicron variants
por: da Costa, Clauber Henrique Souza, et al.
Publicado: (2022)

Interactions of Spike-RBD of SARS-CoV-2 and Platelet Factor 4: New Insights in the Etiopathogenesis of Thrombosis
por: Passariello, Margherita, et al.
Publicado: (2021)

Pilot‐scale process for magnetic bead purification of antibodies directly from non‐clarified CHO cell culture
por: Brechmann, Nils A., et al.
Publicado: (2019)

Neohesperidin and spike RBD interaction in omicron and its sub-variants: In silico, structural and simulation studies
por: Singh, Jaikee Kumar, et al.
Publicado: (2023)

SIM imaging resolves endocytosis of SARS-CoV-2 spike RBD in living cells
por: Miao, Lu, et al.
Publicado: (2023)

Bacillus subtilis spores displaying RBD domain of SARS-CoV-2 spike protein
por: Vetráková, A., et al.
Publicado: (2023)

Effect of Delta and Omicron Mutations on the RBD-SD1 Domain of the Spike Protein in SARS-CoV-2 and the Omicron Mutations on RBD-ACE2 Interface Complex
por: Ching, Wai-Yim, et al.
Publicado: (2022)

Optimizing the production and affinity purification of HIV-1 envelope glycoprotein SOSIP trimers from transiently transfected CHO cells
por: Cupo, Albert, et al.
Publicado: (2019)

Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays
por: Mehalko, Jennifer, et al.
Publicado: (2020)

Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays
por: Mehalko, Jennifer, et al.
Publicado: (2021)

Comparative structural analyses of selected spike protein-RBD mutations in SARS-CoV-2 lineages
por: Roy, Urmi
Publicado: (2021)

Development of multivalent nanobodies blocking SARS-CoV-2 infection by targeting RBD of spike protein
por: Lu, Qizhong, et al.
Publicado: (2021)

Screening and Characterization of Shark-Derived VNARs against SARS-CoV-2 Spike RBD Protein
por: Chen, Yu-Lei, et al.
Publicado: (2022)

Improved Expression of SARS-CoV-2 Spike RBD Using the Insect Cell-Baculovirus System
por: Poodts, Joaquín, et al.
Publicado: (2022)

Anti-Entry Activity of Natural Flavonoids against SARS-CoV-2 by Targeting Spike RBD
por: Meng, Jie-Ru, et al.
Publicado: (2023)

Distinct anti-NP, anti-RBD and anti-Spike antibody profiles discriminate death from survival in COVID-19
por: Servian, Carolina do Prado, et al.
Publicado: (2023)

Production and Purification of LTB-RBD: A Potential Antigen for Mucosal Vaccine Development against SARS-CoV-2
por: Solis-Andrade, Karla I., et al.
Publicado: (2022)

Reduced Intracortical Facilitation to TMS in Both Isolated REM Sleep Behavior Disorder (RBD) and Early Parkinson’s Disease with RBD
por: Lanza, Giuseppe, et al.
Publicado: (2022)

Development and characterization of a novel mAb against bilitranslocase - a new biomarker of renal carcinoma
por: Montanic, Sendi, et al.
Publicado: (2013)

Predictors of RBD progression and conversion to synucleinopathies
por: de Natale, Edoardo Rosario, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_7u4v7fchegcdjlpjej77k8v2op