Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples

Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol...

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Detalles Bibliográficos
Autores principales: Yang, Xiaoqi, Fox, Alisa, Powell, Rebecca L.R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841166/
https://www.ncbi.nlm.nih.gov/pubmed/35284838
http://dx.doi.org/10.1016/j.xpro.2022.101203
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author Yang, Xiaoqi
Fox, Alisa
Powell, Rebecca L.R.
author_facet Yang, Xiaoqi
Fox, Alisa
Powell, Rebecca L.R.
author_sort Yang, Xiaoqi
collection PubMed
description Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol, we describe an optimized indirect ELISA assay aimed to measure SARS-CoV-2-reactive antibodies in human milk, which can be used as a rapid screen on undiluted samples or to designate samples as relatively low, moderate, or high titer. For complete details on the use and execution of this protocol, please refer to Fox et al. (2020).
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spelling pubmed-88411662022-02-14 Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples Yang, Xiaoqi Fox, Alisa Powell, Rebecca L.R. STAR Protoc Protocol Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol, we describe an optimized indirect ELISA assay aimed to measure SARS-CoV-2-reactive antibodies in human milk, which can be used as a rapid screen on undiluted samples or to designate samples as relatively low, moderate, or high titer. For complete details on the use and execution of this protocol, please refer to Fox et al. (2020). Elsevier 2022-02-14 /pmc/articles/PMC8841166/ /pubmed/35284838 http://dx.doi.org/10.1016/j.xpro.2022.101203 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yang, Xiaoqi
Fox, Alisa
Powell, Rebecca L.R.
Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples
title Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples
title_full Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples
title_fullStr Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples
title_full_unstemmed Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples
title_short Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples
title_sort qualitative immunoassay for the detection of anti-sars-cov-2 spike antibody in human milk samples
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841166/
https://www.ncbi.nlm.nih.gov/pubmed/35284838
http://dx.doi.org/10.1016/j.xpro.2022.101203
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