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Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product

Macrophages exhibit high plasticity to achieve their roles in maintaining tissue homeostasis, innate immunity, tissue repair and regeneration. Therefore, macrophages are being evaluated for cell-based therapeutics against inflammatory disorders and cancer. To overcome the limitation related to expan...

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Autores principales: Jo, Hye-Yeong, Seo, Hyang-Hee, Gil, Dayeon, Park, YoungChan, Han, Hyeong-Jun, Han, Hyo-Won, Thimmulappa, Rajesh K., Kim, Sang Cheol, Kim, Jung-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841343/
https://www.ncbi.nlm.nih.gov/pubmed/35173760
http://dx.doi.org/10.3389/fgene.2021.658862
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author Jo, Hye-Yeong
Seo, Hyang-Hee
Gil, Dayeon
Park, YoungChan
Han, Hyeong-Jun
Han, Hyo-Won
Thimmulappa, Rajesh K.
Kim, Sang Cheol
Kim, Jung-Hyun
author_facet Jo, Hye-Yeong
Seo, Hyang-Hee
Gil, Dayeon
Park, YoungChan
Han, Hyeong-Jun
Han, Hyo-Won
Thimmulappa, Rajesh K.
Kim, Sang Cheol
Kim, Jung-Hyun
author_sort Jo, Hye-Yeong
collection PubMed
description Macrophages exhibit high plasticity to achieve their roles in maintaining tissue homeostasis, innate immunity, tissue repair and regeneration. Therefore, macrophages are being evaluated for cell-based therapeutics against inflammatory disorders and cancer. To overcome the limitation related to expansion of primary macrophages and cell numbers, human pluripotent stem cell (hPSC)-derived macrophages are considered as an alternative source of primary macrophages for clinical application. However, the quality of hPSC-derived macrophages with respect to the biological homogeneity remains still unclear. We previously reported a technique to produce hPSC-derived macrophages referred to as iMACs, which is amenable for scale-up. In this study, we have evaluated the biological homogeneity of the iMACs using a transcriptome dataset of 6,230 iMACs obtained by single-cell RNA sequencing. The dataset provides a valuable genomic profile for understanding the molecular characteristics of hPSC-derived macrophage cells and provide a measurement of transcriptomic homogeneity. Our study highlights the usefulness of single cell RNA-seq data in quality control of the cell-based therapy products.
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spelling pubmed-88413432022-02-15 Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product Jo, Hye-Yeong Seo, Hyang-Hee Gil, Dayeon Park, YoungChan Han, Hyeong-Jun Han, Hyo-Won Thimmulappa, Rajesh K. Kim, Sang Cheol Kim, Jung-Hyun Front Genet Genetics Macrophages exhibit high plasticity to achieve their roles in maintaining tissue homeostasis, innate immunity, tissue repair and regeneration. Therefore, macrophages are being evaluated for cell-based therapeutics against inflammatory disorders and cancer. To overcome the limitation related to expansion of primary macrophages and cell numbers, human pluripotent stem cell (hPSC)-derived macrophages are considered as an alternative source of primary macrophages for clinical application. However, the quality of hPSC-derived macrophages with respect to the biological homogeneity remains still unclear. We previously reported a technique to produce hPSC-derived macrophages referred to as iMACs, which is amenable for scale-up. In this study, we have evaluated the biological homogeneity of the iMACs using a transcriptome dataset of 6,230 iMACs obtained by single-cell RNA sequencing. The dataset provides a valuable genomic profile for understanding the molecular characteristics of hPSC-derived macrophage cells and provide a measurement of transcriptomic homogeneity. Our study highlights the usefulness of single cell RNA-seq data in quality control of the cell-based therapy products. Frontiers Media S.A. 2022-01-31 /pmc/articles/PMC8841343/ /pubmed/35173760 http://dx.doi.org/10.3389/fgene.2021.658862 Text en Copyright © 2022 Jo, Seo, Gil, Park, Han, Han, Thimmulappa, Kim and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Jo, Hye-Yeong
Seo, Hyang-Hee
Gil, Dayeon
Park, YoungChan
Han, Hyeong-Jun
Han, Hyo-Won
Thimmulappa, Rajesh K.
Kim, Sang Cheol
Kim, Jung-Hyun
Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product
title Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product
title_full Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product
title_fullStr Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product
title_full_unstemmed Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product
title_short Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product
title_sort single-cell rna sequencing of human pluripotent stem cell-derived macrophages for quality control of the cell therapy product
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841343/
https://www.ncbi.nlm.nih.gov/pubmed/35173760
http://dx.doi.org/10.3389/fgene.2021.658862
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