Cargando…
Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product
Macrophages exhibit high plasticity to achieve their roles in maintaining tissue homeostasis, innate immunity, tissue repair and regeneration. Therefore, macrophages are being evaluated for cell-based therapeutics against inflammatory disorders and cancer. To overcome the limitation related to expan...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841343/ https://www.ncbi.nlm.nih.gov/pubmed/35173760 http://dx.doi.org/10.3389/fgene.2021.658862 |
_version_ | 1784650816358973440 |
---|---|
author | Jo, Hye-Yeong Seo, Hyang-Hee Gil, Dayeon Park, YoungChan Han, Hyeong-Jun Han, Hyo-Won Thimmulappa, Rajesh K. Kim, Sang Cheol Kim, Jung-Hyun |
author_facet | Jo, Hye-Yeong Seo, Hyang-Hee Gil, Dayeon Park, YoungChan Han, Hyeong-Jun Han, Hyo-Won Thimmulappa, Rajesh K. Kim, Sang Cheol Kim, Jung-Hyun |
author_sort | Jo, Hye-Yeong |
collection | PubMed |
description | Macrophages exhibit high plasticity to achieve their roles in maintaining tissue homeostasis, innate immunity, tissue repair and regeneration. Therefore, macrophages are being evaluated for cell-based therapeutics against inflammatory disorders and cancer. To overcome the limitation related to expansion of primary macrophages and cell numbers, human pluripotent stem cell (hPSC)-derived macrophages are considered as an alternative source of primary macrophages for clinical application. However, the quality of hPSC-derived macrophages with respect to the biological homogeneity remains still unclear. We previously reported a technique to produce hPSC-derived macrophages referred to as iMACs, which is amenable for scale-up. In this study, we have evaluated the biological homogeneity of the iMACs using a transcriptome dataset of 6,230 iMACs obtained by single-cell RNA sequencing. The dataset provides a valuable genomic profile for understanding the molecular characteristics of hPSC-derived macrophage cells and provide a measurement of transcriptomic homogeneity. Our study highlights the usefulness of single cell RNA-seq data in quality control of the cell-based therapy products. |
format | Online Article Text |
id | pubmed-8841343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88413432022-02-15 Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product Jo, Hye-Yeong Seo, Hyang-Hee Gil, Dayeon Park, YoungChan Han, Hyeong-Jun Han, Hyo-Won Thimmulappa, Rajesh K. Kim, Sang Cheol Kim, Jung-Hyun Front Genet Genetics Macrophages exhibit high plasticity to achieve their roles in maintaining tissue homeostasis, innate immunity, tissue repair and regeneration. Therefore, macrophages are being evaluated for cell-based therapeutics against inflammatory disorders and cancer. To overcome the limitation related to expansion of primary macrophages and cell numbers, human pluripotent stem cell (hPSC)-derived macrophages are considered as an alternative source of primary macrophages for clinical application. However, the quality of hPSC-derived macrophages with respect to the biological homogeneity remains still unclear. We previously reported a technique to produce hPSC-derived macrophages referred to as iMACs, which is amenable for scale-up. In this study, we have evaluated the biological homogeneity of the iMACs using a transcriptome dataset of 6,230 iMACs obtained by single-cell RNA sequencing. The dataset provides a valuable genomic profile for understanding the molecular characteristics of hPSC-derived macrophage cells and provide a measurement of transcriptomic homogeneity. Our study highlights the usefulness of single cell RNA-seq data in quality control of the cell-based therapy products. Frontiers Media S.A. 2022-01-31 /pmc/articles/PMC8841343/ /pubmed/35173760 http://dx.doi.org/10.3389/fgene.2021.658862 Text en Copyright © 2022 Jo, Seo, Gil, Park, Han, Han, Thimmulappa, Kim and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Jo, Hye-Yeong Seo, Hyang-Hee Gil, Dayeon Park, YoungChan Han, Hyeong-Jun Han, Hyo-Won Thimmulappa, Rajesh K. Kim, Sang Cheol Kim, Jung-Hyun Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product |
title | Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product |
title_full | Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product |
title_fullStr | Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product |
title_full_unstemmed | Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product |
title_short | Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product |
title_sort | single-cell rna sequencing of human pluripotent stem cell-derived macrophages for quality control of the cell therapy product |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841343/ https://www.ncbi.nlm.nih.gov/pubmed/35173760 http://dx.doi.org/10.3389/fgene.2021.658862 |
work_keys_str_mv | AT johyeyeong singlecellrnasequencingofhumanpluripotentstemcellderivedmacrophagesforqualitycontrolofthecelltherapyproduct AT seohyanghee singlecellrnasequencingofhumanpluripotentstemcellderivedmacrophagesforqualitycontrolofthecelltherapyproduct AT gildayeon singlecellrnasequencingofhumanpluripotentstemcellderivedmacrophagesforqualitycontrolofthecelltherapyproduct AT parkyoungchan singlecellrnasequencingofhumanpluripotentstemcellderivedmacrophagesforqualitycontrolofthecelltherapyproduct AT hanhyeongjun singlecellrnasequencingofhumanpluripotentstemcellderivedmacrophagesforqualitycontrolofthecelltherapyproduct AT hanhyowon singlecellrnasequencingofhumanpluripotentstemcellderivedmacrophagesforqualitycontrolofthecelltherapyproduct AT thimmulapparajeshk singlecellrnasequencingofhumanpluripotentstemcellderivedmacrophagesforqualitycontrolofthecelltherapyproduct AT kimsangcheol singlecellrnasequencingofhumanpluripotentstemcellderivedmacrophagesforqualitycontrolofthecelltherapyproduct AT kimjunghyun singlecellrnasequencingofhumanpluripotentstemcellderivedmacrophagesforqualitycontrolofthecelltherapyproduct |