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Evaluation of Acute Supplementation With the Ketone Ester (R)-3-Hydroxybutyl-(R)-3-Hydroxybutyrate (deltaG) in Healthy Volunteers by Cardiac and Skeletal Muscle (31)P Magnetic Resonance Spectroscopy

In this acute intervention study, we investigated the potential benefit of ketone supplementation in humans by studying cardiac phosphocreatine to adenosine-triphosphate ratios (PCr/ATP) and skeletal muscle PCr recovery using phosphorus magnetic resonance spectroscopy ((31)P-MRS) before and after in...

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Autores principales: Cameron, Donnie, Soto-Mota, Adrian, Willis, David R., Ellis, Jane, Procter, Nathan E. K., Greenwood, Richard, Saunders, Neil, Schulte, Rolf F., Vassiliou, Vassilios S., Tyler, Damian J., Schmid, Albrecht Ingo, Rodgers, Christopher T., Malcolm, Paul N., Clarke, Kieran, Frenneaux, Michael P., Valkovič, Ladislav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841822/
https://www.ncbi.nlm.nih.gov/pubmed/35173629
http://dx.doi.org/10.3389/fphys.2022.793987
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author Cameron, Donnie
Soto-Mota, Adrian
Willis, David R.
Ellis, Jane
Procter, Nathan E. K.
Greenwood, Richard
Saunders, Neil
Schulte, Rolf F.
Vassiliou, Vassilios S.
Tyler, Damian J.
Schmid, Albrecht Ingo
Rodgers, Christopher T.
Malcolm, Paul N.
Clarke, Kieran
Frenneaux, Michael P.
Valkovič, Ladislav
author_facet Cameron, Donnie
Soto-Mota, Adrian
Willis, David R.
Ellis, Jane
Procter, Nathan E. K.
Greenwood, Richard
Saunders, Neil
Schulte, Rolf F.
Vassiliou, Vassilios S.
Tyler, Damian J.
Schmid, Albrecht Ingo
Rodgers, Christopher T.
Malcolm, Paul N.
Clarke, Kieran
Frenneaux, Michael P.
Valkovič, Ladislav
author_sort Cameron, Donnie
collection PubMed
description In this acute intervention study, we investigated the potential benefit of ketone supplementation in humans by studying cardiac phosphocreatine to adenosine-triphosphate ratios (PCr/ATP) and skeletal muscle PCr recovery using phosphorus magnetic resonance spectroscopy ((31)P-MRS) before and after ingestion of a ketone ester drink. We recruited 28 healthy individuals: 12 aged 23–70 years for cardiac (31)P-MRS, and 16 aged 60–75 years for skeletal muscle (31)P-MRS. Baseline and post-intervention resting cardiac and dynamic skeletal muscle (31)P-MRS scans were performed in one visit, where 25 g of the ketone monoester, deltaG(®), was administered after the baseline scan. Administration was timed so that post-intervention (31)P-MRS would take place 30 min after deltaG(®) ingestion. The deltaG(®) ketone drink was well-tolerated by all participants. In participants who provided blood samples, post-intervention blood glucose, lactate and non-esterified fatty acid concentrations decreased significantly (−28.8%, p ≪ 0.001; −28.2%, p = 0.02; and −49.1%, p ≪ 0.001, respectively), while levels of the ketone body D-beta-hydroxybutyrate significantly increased from mean (standard deviation) 0.7 (0.3) to 4.0 (1.1) mmol/L after 30 min (p ≪ 0.001). There were no significant changes in cardiac PCr/ATP or skeletal muscle metabolic parameters between baseline and post-intervention. Acute ketone supplementation caused mild ketosis in blood, with drops in glucose, lactate, and free fatty acids; however, such changes were not associated with changes in (31)P-MRS measures in the heart or in skeletal muscle. Future work may focus on the effect of longer-term ketone supplementation on tissue energetics in groups with compromised mitochondrial function.
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spelling pubmed-88418222022-02-15 Evaluation of Acute Supplementation With the Ketone Ester (R)-3-Hydroxybutyl-(R)-3-Hydroxybutyrate (deltaG) in Healthy Volunteers by Cardiac and Skeletal Muscle (31)P Magnetic Resonance Spectroscopy Cameron, Donnie Soto-Mota, Adrian Willis, David R. Ellis, Jane Procter, Nathan E. K. Greenwood, Richard Saunders, Neil Schulte, Rolf F. Vassiliou, Vassilios S. Tyler, Damian J. Schmid, Albrecht Ingo Rodgers, Christopher T. Malcolm, Paul N. Clarke, Kieran Frenneaux, Michael P. Valkovič, Ladislav Front Physiol Physiology In this acute intervention study, we investigated the potential benefit of ketone supplementation in humans by studying cardiac phosphocreatine to adenosine-triphosphate ratios (PCr/ATP) and skeletal muscle PCr recovery using phosphorus magnetic resonance spectroscopy ((31)P-MRS) before and after ingestion of a ketone ester drink. We recruited 28 healthy individuals: 12 aged 23–70 years for cardiac (31)P-MRS, and 16 aged 60–75 years for skeletal muscle (31)P-MRS. Baseline and post-intervention resting cardiac and dynamic skeletal muscle (31)P-MRS scans were performed in one visit, where 25 g of the ketone monoester, deltaG(®), was administered after the baseline scan. Administration was timed so that post-intervention (31)P-MRS would take place 30 min after deltaG(®) ingestion. The deltaG(®) ketone drink was well-tolerated by all participants. In participants who provided blood samples, post-intervention blood glucose, lactate and non-esterified fatty acid concentrations decreased significantly (−28.8%, p ≪ 0.001; −28.2%, p = 0.02; and −49.1%, p ≪ 0.001, respectively), while levels of the ketone body D-beta-hydroxybutyrate significantly increased from mean (standard deviation) 0.7 (0.3) to 4.0 (1.1) mmol/L after 30 min (p ≪ 0.001). There were no significant changes in cardiac PCr/ATP or skeletal muscle metabolic parameters between baseline and post-intervention. Acute ketone supplementation caused mild ketosis in blood, with drops in glucose, lactate, and free fatty acids; however, such changes were not associated with changes in (31)P-MRS measures in the heart or in skeletal muscle. Future work may focus on the effect of longer-term ketone supplementation on tissue energetics in groups with compromised mitochondrial function. Frontiers Media S.A. 2022-01-31 /pmc/articles/PMC8841822/ /pubmed/35173629 http://dx.doi.org/10.3389/fphys.2022.793987 Text en Copyright © 2022 Cameron, Soto-Mota, Willis, Ellis, Procter, Greenwood, Saunders, Schulte, Vassiliou, Tyler, Schmid, Rodgers, Malcolm, Clarke, Frenneaux and Valkovič. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Cameron, Donnie
Soto-Mota, Adrian
Willis, David R.
Ellis, Jane
Procter, Nathan E. K.
Greenwood, Richard
Saunders, Neil
Schulte, Rolf F.
Vassiliou, Vassilios S.
Tyler, Damian J.
Schmid, Albrecht Ingo
Rodgers, Christopher T.
Malcolm, Paul N.
Clarke, Kieran
Frenneaux, Michael P.
Valkovič, Ladislav
Evaluation of Acute Supplementation With the Ketone Ester (R)-3-Hydroxybutyl-(R)-3-Hydroxybutyrate (deltaG) in Healthy Volunteers by Cardiac and Skeletal Muscle (31)P Magnetic Resonance Spectroscopy
title Evaluation of Acute Supplementation With the Ketone Ester (R)-3-Hydroxybutyl-(R)-3-Hydroxybutyrate (deltaG) in Healthy Volunteers by Cardiac and Skeletal Muscle (31)P Magnetic Resonance Spectroscopy
title_full Evaluation of Acute Supplementation With the Ketone Ester (R)-3-Hydroxybutyl-(R)-3-Hydroxybutyrate (deltaG) in Healthy Volunteers by Cardiac and Skeletal Muscle (31)P Magnetic Resonance Spectroscopy
title_fullStr Evaluation of Acute Supplementation With the Ketone Ester (R)-3-Hydroxybutyl-(R)-3-Hydroxybutyrate (deltaG) in Healthy Volunteers by Cardiac and Skeletal Muscle (31)P Magnetic Resonance Spectroscopy
title_full_unstemmed Evaluation of Acute Supplementation With the Ketone Ester (R)-3-Hydroxybutyl-(R)-3-Hydroxybutyrate (deltaG) in Healthy Volunteers by Cardiac and Skeletal Muscle (31)P Magnetic Resonance Spectroscopy
title_short Evaluation of Acute Supplementation With the Ketone Ester (R)-3-Hydroxybutyl-(R)-3-Hydroxybutyrate (deltaG) in Healthy Volunteers by Cardiac and Skeletal Muscle (31)P Magnetic Resonance Spectroscopy
title_sort evaluation of acute supplementation with the ketone ester (r)-3-hydroxybutyl-(r)-3-hydroxybutyrate (deltag) in healthy volunteers by cardiac and skeletal muscle (31)p magnetic resonance spectroscopy
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841822/
https://www.ncbi.nlm.nih.gov/pubmed/35173629
http://dx.doi.org/10.3389/fphys.2022.793987
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