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RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection
Takifugu rubripes is commonly subjected to the disease-causing bacterium, Vibrio harveyi. However, the mechanism involved in the immune response of T. rubripes to V. harveyi infection is unclear. We conducted a transcriptomic analysis of the spleen and gill from T. rubripes infected with V. harveyi....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841829/ https://www.ncbi.nlm.nih.gov/pubmed/35174239 http://dx.doi.org/10.3389/fvets.2021.813988 |
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author | Gao, Dongxu Lei, Wei Wang, Chenshi Ni, Ping Cui, Xiaoyu Huang, Xindi Ye, Shigen |
author_facet | Gao, Dongxu Lei, Wei Wang, Chenshi Ni, Ping Cui, Xiaoyu Huang, Xindi Ye, Shigen |
author_sort | Gao, Dongxu |
collection | PubMed |
description | Takifugu rubripes is commonly subjected to the disease-causing bacterium, Vibrio harveyi. However, the mechanism involved in the immune response of T. rubripes to V. harveyi infection is unclear. We conducted a transcriptomic analysis of the spleen and gill from T. rubripes infected with V. harveyi. We obtained 60,981,357 and 60,760,550 clean reads from the control and infected spleens, and 57,407,586 and 57,536,651 clean reads from the control and infected gills, respectively. We also identified 1,560 and 1,213 differentially expressed genes in the spleen and gill, respectively. Gene ontology analysis revealed that the most enriched biological process in both the spleen and gill was “immune response”. The most enriched Kyoto Encyclopedia of Genes and Genomes immune response–related pathways were the NOD-like receptor signaling pathway in the spleen and cytokine–cytokine receptor interaction in the gill. We found 10 candidate immune-related genes in the spleen and gill. These putative immune pathways and candidate genes will provide insight into the immune response mechanisms of T. rubripes against V. harveyi. |
format | Online Article Text |
id | pubmed-8841829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88418292022-02-15 RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection Gao, Dongxu Lei, Wei Wang, Chenshi Ni, Ping Cui, Xiaoyu Huang, Xindi Ye, Shigen Front Vet Sci Veterinary Science Takifugu rubripes is commonly subjected to the disease-causing bacterium, Vibrio harveyi. However, the mechanism involved in the immune response of T. rubripes to V. harveyi infection is unclear. We conducted a transcriptomic analysis of the spleen and gill from T. rubripes infected with V. harveyi. We obtained 60,981,357 and 60,760,550 clean reads from the control and infected spleens, and 57,407,586 and 57,536,651 clean reads from the control and infected gills, respectively. We also identified 1,560 and 1,213 differentially expressed genes in the spleen and gill, respectively. Gene ontology analysis revealed that the most enriched biological process in both the spleen and gill was “immune response”. The most enriched Kyoto Encyclopedia of Genes and Genomes immune response–related pathways were the NOD-like receptor signaling pathway in the spleen and cytokine–cytokine receptor interaction in the gill. We found 10 candidate immune-related genes in the spleen and gill. These putative immune pathways and candidate genes will provide insight into the immune response mechanisms of T. rubripes against V. harveyi. Frontiers Media S.A. 2022-01-31 /pmc/articles/PMC8841829/ /pubmed/35174239 http://dx.doi.org/10.3389/fvets.2021.813988 Text en Copyright © 2022 Gao, Lei, Wang, Ni, Cui, Huang and Ye. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Gao, Dongxu Lei, Wei Wang, Chenshi Ni, Ping Cui, Xiaoyu Huang, Xindi Ye, Shigen RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection |
title | RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection |
title_full | RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection |
title_fullStr | RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection |
title_full_unstemmed | RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection |
title_short | RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection |
title_sort | rna-sequencing analysis of the spleen and gill of takifugu rubripes in response to vibrio harveyi infection |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841829/ https://www.ncbi.nlm.nih.gov/pubmed/35174239 http://dx.doi.org/10.3389/fvets.2021.813988 |
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