CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens

The cuticle protein (CP) encoded by CPR63 plays a role in deltamethrin resistance in Culex pipiens pallens. Herein, we investigated the distribution of CPR63 transcripts in this organism and observed high expression levels in legs and wings. Furthermore, expression of CPR63 in the legs of deltamethr...

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Autores principales: Xu, Yang, Xu, Jingwei, Zhou, Yang, Li, Xixi, Meng, Yufen, Ma, Lei, Zhou, Dan, Shen, Bo, Sun, Yan, Zhu, Changliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8842966/
https://www.ncbi.nlm.nih.gov/pubmed/35164827
http://dx.doi.org/10.1186/s13071-022-05175-0
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author Xu, Yang
Xu, Jingwei
Zhou, Yang
Li, Xixi
Meng, Yufen
Ma, Lei
Zhou, Dan
Shen, Bo
Sun, Yan
Zhu, Changliang
author_facet Xu, Yang
Xu, Jingwei
Zhou, Yang
Li, Xixi
Meng, Yufen
Ma, Lei
Zhou, Dan
Shen, Bo
Sun, Yan
Zhu, Changliang
author_sort Xu, Yang
collection PubMed
description The cuticle protein (CP) encoded by CPR63 plays a role in deltamethrin resistance in Culex pipiens pallens. Herein, we investigated the distribution of CPR63 transcripts in this organism and observed high expression levels in legs and wings. Furthermore, expression of CPR63 in the legs of deltamethrin-resistant (DR) strains was 2.17-fold higher than in deltamethrin-susceptible (DS) strains. Cuticle analysis of small interfering RNA (siRNA) groups by scanning electron microscopy (SEM) revealed a significantly thinner cuticle of the tarsi in the siCPR63 group than in the siNC (negative control siRNA) group. Transmission electron microscopy (TEM) revealed that the exocuticle and endocuticle thickness of the tarsi were significantly thinner, which contributes the thinner procuticle of tarsi in the siCPR63 group than in the siNC group. Our results suggested that CPR63 might contribute to the resistance phenotype by thickening the cuticle and thereby possibly increasing the tolerance of mosquitoes to deltamethrin. [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-022-05175-0.
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spelling pubmed-88429662022-02-16 CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens Xu, Yang Xu, Jingwei Zhou, Yang Li, Xixi Meng, Yufen Ma, Lei Zhou, Dan Shen, Bo Sun, Yan Zhu, Changliang Parasit Vectors Research The cuticle protein (CP) encoded by CPR63 plays a role in deltamethrin resistance in Culex pipiens pallens. Herein, we investigated the distribution of CPR63 transcripts in this organism and observed high expression levels in legs and wings. Furthermore, expression of CPR63 in the legs of deltamethrin-resistant (DR) strains was 2.17-fold higher than in deltamethrin-susceptible (DS) strains. Cuticle analysis of small interfering RNA (siRNA) groups by scanning electron microscopy (SEM) revealed a significantly thinner cuticle of the tarsi in the siCPR63 group than in the siNC (negative control siRNA) group. Transmission electron microscopy (TEM) revealed that the exocuticle and endocuticle thickness of the tarsi were significantly thinner, which contributes the thinner procuticle of tarsi in the siCPR63 group than in the siNC group. Our results suggested that CPR63 might contribute to the resistance phenotype by thickening the cuticle and thereby possibly increasing the tolerance of mosquitoes to deltamethrin. [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-022-05175-0. BioMed Central 2022-02-14 /pmc/articles/PMC8842966/ /pubmed/35164827 http://dx.doi.org/10.1186/s13071-022-05175-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Xu, Yang
Xu, Jingwei
Zhou, Yang
Li, Xixi
Meng, Yufen
Ma, Lei
Zhou, Dan
Shen, Bo
Sun, Yan
Zhu, Changliang
CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens
title CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens
title_full CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens
title_fullStr CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens
title_full_unstemmed CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens
title_short CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens
title_sort cpr63 promotes pyrethroid resistance by increasing cuticle thickness in culex pipiens pallens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8842966/
https://www.ncbi.nlm.nih.gov/pubmed/35164827
http://dx.doi.org/10.1186/s13071-022-05175-0
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