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Effects of Antibiotic Use on Saliva Antibody Content and Oral Microbiota in Sprague Dawley Rats
Antibiotics are often used to treat systemic diseases not associated with the oral cavity. This application of antibiotics may affect the healthy oral microbiota community, as it destroys the balance between specific bacterial populations throughout the ecosystem and may lead to dysbacteriosis. We h...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843035/ https://www.ncbi.nlm.nih.gov/pubmed/35174102 http://dx.doi.org/10.3389/fcimb.2022.721691 |
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author | Cheng, Xi He, Fuming Si, Misi Sun, Ping Chen, Qianming |
author_facet | Cheng, Xi He, Fuming Si, Misi Sun, Ping Chen, Qianming |
author_sort | Cheng, Xi |
collection | PubMed |
description | Antibiotics are often used to treat systemic diseases not associated with the oral cavity. This application of antibiotics may affect the healthy oral microbiota community, as it destroys the balance between specific bacterial populations throughout the ecosystem and may lead to dysbacteriosis. We hypothesized that the effects on antibiotics on oral microbiota regulation and function would affect antibody content in saliva, depending on the antibiotic type. To address this, a total of 24 Sprague Dawley rats (divided into 4 cages, 6 per pen) were administered amoxicillin (AMX), spiramycin (SP), metronidazole (MTZ), or water (control) daily for 14 days (gavage). After treatment was completed, high-throughput sequencing of 16S rRNA genes was used to determine changes in the composition, metabolic function, and diversity of oral microbiota in the rats. Enzyme-linked immunosorbent assay was used to detect antibodies in saliva, including SIgA, IgG, and IgM. Results showed that AMX, MTZ, and SP significantly affected oral microbiota composition, diversity, and metabolic function in rats. AMX induced substantial changes in the rat salivary antibody concentrations. At the genus level, the relative abundance of Rothia and Haemophilus was higher in the AMX group than in the other groups. In conclusion, antibiotics-induced changes in oral microbiota populations may be associated with changes in salivary antibody concentrations. However, the specific interaction mechanisms remain unknown, and it is still unclear whether significant changes in the oral microbiota cause changes in salivary antibody concentrations or vice versa. |
format | Online Article Text |
id | pubmed-8843035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88430352022-02-15 Effects of Antibiotic Use on Saliva Antibody Content and Oral Microbiota in Sprague Dawley Rats Cheng, Xi He, Fuming Si, Misi Sun, Ping Chen, Qianming Front Cell Infect Microbiol Cellular and Infection Microbiology Antibiotics are often used to treat systemic diseases not associated with the oral cavity. This application of antibiotics may affect the healthy oral microbiota community, as it destroys the balance between specific bacterial populations throughout the ecosystem and may lead to dysbacteriosis. We hypothesized that the effects on antibiotics on oral microbiota regulation and function would affect antibody content in saliva, depending on the antibiotic type. To address this, a total of 24 Sprague Dawley rats (divided into 4 cages, 6 per pen) were administered amoxicillin (AMX), spiramycin (SP), metronidazole (MTZ), or water (control) daily for 14 days (gavage). After treatment was completed, high-throughput sequencing of 16S rRNA genes was used to determine changes in the composition, metabolic function, and diversity of oral microbiota in the rats. Enzyme-linked immunosorbent assay was used to detect antibodies in saliva, including SIgA, IgG, and IgM. Results showed that AMX, MTZ, and SP significantly affected oral microbiota composition, diversity, and metabolic function in rats. AMX induced substantial changes in the rat salivary antibody concentrations. At the genus level, the relative abundance of Rothia and Haemophilus was higher in the AMX group than in the other groups. In conclusion, antibiotics-induced changes in oral microbiota populations may be associated with changes in salivary antibody concentrations. However, the specific interaction mechanisms remain unknown, and it is still unclear whether significant changes in the oral microbiota cause changes in salivary antibody concentrations or vice versa. Frontiers Media S.A. 2022-01-31 /pmc/articles/PMC8843035/ /pubmed/35174102 http://dx.doi.org/10.3389/fcimb.2022.721691 Text en Copyright © 2022 Cheng, He, Si, Sun and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Cheng, Xi He, Fuming Si, Misi Sun, Ping Chen, Qianming Effects of Antibiotic Use on Saliva Antibody Content and Oral Microbiota in Sprague Dawley Rats |
title | Effects of Antibiotic Use on Saliva Antibody Content and Oral Microbiota in Sprague Dawley Rats |
title_full | Effects of Antibiotic Use on Saliva Antibody Content and Oral Microbiota in Sprague Dawley Rats |
title_fullStr | Effects of Antibiotic Use on Saliva Antibody Content and Oral Microbiota in Sprague Dawley Rats |
title_full_unstemmed | Effects of Antibiotic Use on Saliva Antibody Content and Oral Microbiota in Sprague Dawley Rats |
title_short | Effects of Antibiotic Use on Saliva Antibody Content and Oral Microbiota in Sprague Dawley Rats |
title_sort | effects of antibiotic use on saliva antibody content and oral microbiota in sprague dawley rats |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843035/ https://www.ncbi.nlm.nih.gov/pubmed/35174102 http://dx.doi.org/10.3389/fcimb.2022.721691 |
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