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Three-dimensional multi-site random access photostimulation (3D-MAP)

Optical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-p...

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Detalles Bibliográficos
Autores principales: Xue, Yi, Waller, Laura, Adesnik, Hillel, Pégard, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843094/
https://www.ncbi.nlm.nih.gov/pubmed/35156923
http://dx.doi.org/10.7554/eLife.73266
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author Xue, Yi
Waller, Laura
Adesnik, Hillel
Pégard, Nicolas
author_facet Xue, Yi
Waller, Laura
Adesnik, Hillel
Pégard, Nicolas
author_sort Xue, Yi
collection PubMed
description Optical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-power illumination that only addresses relatively small populations of neurons in parallel. Conversely, one-photon holographic techniques can stimulate more neurons with two to three orders lower power, but with limited resolution or addressable volume. Perhaps most problematically, two-photon holographic optogenetic systems are extremely expensive and sophisticated which has precluded their broader adoption in the neuroscience community. To address this technical gap, we introduce a new one-photon light sculpting technique, three-dimensional multi-site random access photostimulation (3D-MAP), that overcomes these limitations by modulating light dynamically, both in the spatial and in the angular domain at multi-kHz rates. We use 3D-MAP to interrogate neural circuits in 3D and demonstrate simultaneous photostimulation and imaging of dozens of user-selected neurons in the intact mouse brain in vivo with high spatio-temporal resolution. 3D-MAP can be broadly adopted for high-throughput all-optical interrogation of brain circuits owing to its powerful combination of scale, speed, simplicity, and cost.
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spelling pubmed-88430942022-02-16 Three-dimensional multi-site random access photostimulation (3D-MAP) Xue, Yi Waller, Laura Adesnik, Hillel Pégard, Nicolas eLife Neuroscience Optical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-power illumination that only addresses relatively small populations of neurons in parallel. Conversely, one-photon holographic techniques can stimulate more neurons with two to three orders lower power, but with limited resolution or addressable volume. Perhaps most problematically, two-photon holographic optogenetic systems are extremely expensive and sophisticated which has precluded their broader adoption in the neuroscience community. To address this technical gap, we introduce a new one-photon light sculpting technique, three-dimensional multi-site random access photostimulation (3D-MAP), that overcomes these limitations by modulating light dynamically, both in the spatial and in the angular domain at multi-kHz rates. We use 3D-MAP to interrogate neural circuits in 3D and demonstrate simultaneous photostimulation and imaging of dozens of user-selected neurons in the intact mouse brain in vivo with high spatio-temporal resolution. 3D-MAP can be broadly adopted for high-throughput all-optical interrogation of brain circuits owing to its powerful combination of scale, speed, simplicity, and cost. eLife Sciences Publications, Ltd 2022-02-14 /pmc/articles/PMC8843094/ /pubmed/35156923 http://dx.doi.org/10.7554/eLife.73266 Text en © 2022, Xue et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Xue, Yi
Waller, Laura
Adesnik, Hillel
Pégard, Nicolas
Three-dimensional multi-site random access photostimulation (3D-MAP)
title Three-dimensional multi-site random access photostimulation (3D-MAP)
title_full Three-dimensional multi-site random access photostimulation (3D-MAP)
title_fullStr Three-dimensional multi-site random access photostimulation (3D-MAP)
title_full_unstemmed Three-dimensional multi-site random access photostimulation (3D-MAP)
title_short Three-dimensional multi-site random access photostimulation (3D-MAP)
title_sort three-dimensional multi-site random access photostimulation (3d-map)
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843094/
https://www.ncbi.nlm.nih.gov/pubmed/35156923
http://dx.doi.org/10.7554/eLife.73266
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