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Three-dimensional multi-site random access photostimulation (3D-MAP)
Optical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-p...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843094/ https://www.ncbi.nlm.nih.gov/pubmed/35156923 http://dx.doi.org/10.7554/eLife.73266 |
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author | Xue, Yi Waller, Laura Adesnik, Hillel Pégard, Nicolas |
author_facet | Xue, Yi Waller, Laura Adesnik, Hillel Pégard, Nicolas |
author_sort | Xue, Yi |
collection | PubMed |
description | Optical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-power illumination that only addresses relatively small populations of neurons in parallel. Conversely, one-photon holographic techniques can stimulate more neurons with two to three orders lower power, but with limited resolution or addressable volume. Perhaps most problematically, two-photon holographic optogenetic systems are extremely expensive and sophisticated which has precluded their broader adoption in the neuroscience community. To address this technical gap, we introduce a new one-photon light sculpting technique, three-dimensional multi-site random access photostimulation (3D-MAP), that overcomes these limitations by modulating light dynamically, both in the spatial and in the angular domain at multi-kHz rates. We use 3D-MAP to interrogate neural circuits in 3D and demonstrate simultaneous photostimulation and imaging of dozens of user-selected neurons in the intact mouse brain in vivo with high spatio-temporal resolution. 3D-MAP can be broadly adopted for high-throughput all-optical interrogation of brain circuits owing to its powerful combination of scale, speed, simplicity, and cost. |
format | Online Article Text |
id | pubmed-8843094 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-88430942022-02-16 Three-dimensional multi-site random access photostimulation (3D-MAP) Xue, Yi Waller, Laura Adesnik, Hillel Pégard, Nicolas eLife Neuroscience Optical control of neural ensemble activity is crucial for understanding brain function and disease, yet no technology can achieve optogenetic control of very large numbers of neurons at an extremely fast rate over a large volume. State-of-the-art multiphoton holographic optogenetics requires high-power illumination that only addresses relatively small populations of neurons in parallel. Conversely, one-photon holographic techniques can stimulate more neurons with two to three orders lower power, but with limited resolution or addressable volume. Perhaps most problematically, two-photon holographic optogenetic systems are extremely expensive and sophisticated which has precluded their broader adoption in the neuroscience community. To address this technical gap, we introduce a new one-photon light sculpting technique, three-dimensional multi-site random access photostimulation (3D-MAP), that overcomes these limitations by modulating light dynamically, both in the spatial and in the angular domain at multi-kHz rates. We use 3D-MAP to interrogate neural circuits in 3D and demonstrate simultaneous photostimulation and imaging of dozens of user-selected neurons in the intact mouse brain in vivo with high spatio-temporal resolution. 3D-MAP can be broadly adopted for high-throughput all-optical interrogation of brain circuits owing to its powerful combination of scale, speed, simplicity, and cost. eLife Sciences Publications, Ltd 2022-02-14 /pmc/articles/PMC8843094/ /pubmed/35156923 http://dx.doi.org/10.7554/eLife.73266 Text en © 2022, Xue et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Xue, Yi Waller, Laura Adesnik, Hillel Pégard, Nicolas Three-dimensional multi-site random access photostimulation (3D-MAP) |
title | Three-dimensional multi-site random access photostimulation (3D-MAP) |
title_full | Three-dimensional multi-site random access photostimulation (3D-MAP) |
title_fullStr | Three-dimensional multi-site random access photostimulation (3D-MAP) |
title_full_unstemmed | Three-dimensional multi-site random access photostimulation (3D-MAP) |
title_short | Three-dimensional multi-site random access photostimulation (3D-MAP) |
title_sort | three-dimensional multi-site random access photostimulation (3d-map) |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843094/ https://www.ncbi.nlm.nih.gov/pubmed/35156923 http://dx.doi.org/10.7554/eLife.73266 |
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