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Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma

Diffuse intrinsic pontine glioma (DIPG) is a lethal pediatric brain tumor. While there are a number of in vivo rodent models for evaluating tumor biology and response to therapy, these models require significant time and resources. Here, we established the chick-embryo chorioallantoic (CAM) assay as...

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Autores principales: Power, Erica A., Fernandez-Torres, Jenelys, Zhang, Liang, Yaun, Ruiyi, Lucien, Fabrice, Daniels, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843199/
https://www.ncbi.nlm.nih.gov/pubmed/35157705
http://dx.doi.org/10.1371/journal.pone.0263822
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author Power, Erica A.
Fernandez-Torres, Jenelys
Zhang, Liang
Yaun, Ruiyi
Lucien, Fabrice
Daniels, David J.
author_facet Power, Erica A.
Fernandez-Torres, Jenelys
Zhang, Liang
Yaun, Ruiyi
Lucien, Fabrice
Daniels, David J.
author_sort Power, Erica A.
collection PubMed
description Diffuse intrinsic pontine glioma (DIPG) is a lethal pediatric brain tumor. While there are a number of in vivo rodent models for evaluating tumor biology and response to therapy, these models require significant time and resources. Here, we established the chick-embryo chorioallantoic (CAM) assay as an affordable and time efficient xenograft model for testing a variety of treatment approaches for DIPG. We found that patient-derived DIPG tumors develop in the CAM and maintain the same genetic and epigenetic characteristics of native DIPG tumors. We monitored tumor response to pharmaco- and radiation therapy by 3-D ultrasound volumetric and vasculature analysis. In this study, we established and validated the CAM model as a potential intermediate xenograft model for DIPG and its use for testing novel treatment approaches that include pharmacotherapy or radiation.
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spelling pubmed-88431992022-02-15 Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma Power, Erica A. Fernandez-Torres, Jenelys Zhang, Liang Yaun, Ruiyi Lucien, Fabrice Daniels, David J. PLoS One Research Article Diffuse intrinsic pontine glioma (DIPG) is a lethal pediatric brain tumor. While there are a number of in vivo rodent models for evaluating tumor biology and response to therapy, these models require significant time and resources. Here, we established the chick-embryo chorioallantoic (CAM) assay as an affordable and time efficient xenograft model for testing a variety of treatment approaches for DIPG. We found that patient-derived DIPG tumors develop in the CAM and maintain the same genetic and epigenetic characteristics of native DIPG tumors. We monitored tumor response to pharmaco- and radiation therapy by 3-D ultrasound volumetric and vasculature analysis. In this study, we established and validated the CAM model as a potential intermediate xenograft model for DIPG and its use for testing novel treatment approaches that include pharmacotherapy or radiation. Public Library of Science 2022-02-14 /pmc/articles/PMC8843199/ /pubmed/35157705 http://dx.doi.org/10.1371/journal.pone.0263822 Text en © 2022 Power et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Power, Erica A.
Fernandez-Torres, Jenelys
Zhang, Liang
Yaun, Ruiyi
Lucien, Fabrice
Daniels, David J.
Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma
title Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma
title_full Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma
title_fullStr Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma
title_full_unstemmed Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma
title_short Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma
title_sort chorioallantoic membrane (cam) assay to study treatment effects in diffuse intrinsic pontine glioma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843199/
https://www.ncbi.nlm.nih.gov/pubmed/35157705
http://dx.doi.org/10.1371/journal.pone.0263822
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