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Evaluation of PCR pncA-restriction fragment length polymorphism and PCR amplification of genomic regions of difference for the identification of M. bovis strains in lymph nodes cultures

BACKGROUND: A rapid accurate identification of Mycobacterium bovis is essential for surveillance purposes. OBJECTIVES: A PCR pncA-Restriction Fragment Length Polymorphism (RFLP) and a multiplex PCR based on the detection of 3 regions of difference (RD-PCR): RD9, RD4 and RD1 were evaluated for the id...

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Detalles Bibliográficos
Autores principales: Bouzouita, Imen, Draoui, Henda, Mahdhi, Samia, Essalah, Leila, Saidi, Leila Slim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Makerere Medical School 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843293/
https://www.ncbi.nlm.nih.gov/pubmed/35222558
http://dx.doi.org/10.4314/ahs.v21i3.4