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Cytokinesis During the First Division of a Mouse Embryo

Cell division consists of nuclear division (mitosis for somatic cells and meiosis for germ cells) and cytoplasmic division (cytokinesis). Embryonic developments are highly programmed, and thus, each cellular event during early embryo development is stable. For mouse embryos, the first time of mitosi...

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Autores principales: Fan, Xiao-Yan, Wang, Xing-Hua, Xie, Feng-Yun, Ma, Jun-Yu, Ou, Xiang-Hong, Luo, Shi-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843819/
https://www.ncbi.nlm.nih.gov/pubmed/35178404
http://dx.doi.org/10.3389/fcell.2021.815599
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author Fan, Xiao-Yan
Wang, Xing-Hua
Xie, Feng-Yun
Ma, Jun-Yu
Ou, Xiang-Hong
Luo, Shi-Ming
author_facet Fan, Xiao-Yan
Wang, Xing-Hua
Xie, Feng-Yun
Ma, Jun-Yu
Ou, Xiang-Hong
Luo, Shi-Ming
author_sort Fan, Xiao-Yan
collection PubMed
description Cell division consists of nuclear division (mitosis for somatic cells and meiosis for germ cells) and cytoplasmic division (cytokinesis). Embryonic developments are highly programmed, and thus, each cellular event during early embryo development is stable. For mouse embryos, the first time of mitosis is completed about 22 h after fertilization. However, it remains unclear when the embryo completes its first cytokinesis. Here, we microinjected only one cell in the 2-cell stage mouse embryos with mRNA, which encodes green fluorescence protein (GFP). By monitoring the GFP protein transport dynamics between the two cells, we demonstrated that the first time of cytokinesis in mouse embryos is completed about 15 h after mitosis, namely 37 h after fertilization. In addition, our results indicate that the cytoplasmic protein transport between daughter cells is very effective, which relies on microtubules instead of microfilaments in 2-cell mouse embryos. These results should enrich people’s understanding of the first cell division and cytoskeleton in mouse embryos and then learn more about the mechanisms of early embryo development in mammals.
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spelling pubmed-88438192022-02-16 Cytokinesis During the First Division of a Mouse Embryo Fan, Xiao-Yan Wang, Xing-Hua Xie, Feng-Yun Ma, Jun-Yu Ou, Xiang-Hong Luo, Shi-Ming Front Cell Dev Biol Cell and Developmental Biology Cell division consists of nuclear division (mitosis for somatic cells and meiosis for germ cells) and cytoplasmic division (cytokinesis). Embryonic developments are highly programmed, and thus, each cellular event during early embryo development is stable. For mouse embryos, the first time of mitosis is completed about 22 h after fertilization. However, it remains unclear when the embryo completes its first cytokinesis. Here, we microinjected only one cell in the 2-cell stage mouse embryos with mRNA, which encodes green fluorescence protein (GFP). By monitoring the GFP protein transport dynamics between the two cells, we demonstrated that the first time of cytokinesis in mouse embryos is completed about 15 h after mitosis, namely 37 h after fertilization. In addition, our results indicate that the cytoplasmic protein transport between daughter cells is very effective, which relies on microtubules instead of microfilaments in 2-cell mouse embryos. These results should enrich people’s understanding of the first cell division and cytoskeleton in mouse embryos and then learn more about the mechanisms of early embryo development in mammals. Frontiers Media S.A. 2022-02-01 /pmc/articles/PMC8843819/ /pubmed/35178404 http://dx.doi.org/10.3389/fcell.2021.815599 Text en Copyright © 2022 Fan, Wang, Xie, Ma, Ou and Luo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Fan, Xiao-Yan
Wang, Xing-Hua
Xie, Feng-Yun
Ma, Jun-Yu
Ou, Xiang-Hong
Luo, Shi-Ming
Cytokinesis During the First Division of a Mouse Embryo
title Cytokinesis During the First Division of a Mouse Embryo
title_full Cytokinesis During the First Division of a Mouse Embryo
title_fullStr Cytokinesis During the First Division of a Mouse Embryo
title_full_unstemmed Cytokinesis During the First Division of a Mouse Embryo
title_short Cytokinesis During the First Division of a Mouse Embryo
title_sort cytokinesis during the first division of a mouse embryo
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843819/
https://www.ncbi.nlm.nih.gov/pubmed/35178404
http://dx.doi.org/10.3389/fcell.2021.815599
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