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Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages

[PURPOSE]: In this study, we investigated whether a 70% ethanolic (EtOH) extract of Sargassum horneri had antioxidant and anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated macrophage-like RAW 264.7 cells. [METHODS]: The proximate composition, fatty acids, amino acids, and dietary fibe...

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Autores principales: Kim, Min Ju, Jo, Hee Geun, Ramakrishna, Chilakala, Lee, Seung-Jae, Lee, Dong-Sung, Cheong, Sun Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Exercise Nutrition 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843843/
https://www.ncbi.nlm.nih.gov/pubmed/35152623
http://dx.doi.org/10.20463/pan.2021.0025
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author Kim, Min Ju
Jo, Hee Geun
Ramakrishna, Chilakala
Lee, Seung-Jae
Lee, Dong-Sung
Cheong, Sun Hee
author_facet Kim, Min Ju
Jo, Hee Geun
Ramakrishna, Chilakala
Lee, Seung-Jae
Lee, Dong-Sung
Cheong, Sun Hee
author_sort Kim, Min Ju
collection PubMed
description [PURPOSE]: In this study, we investigated whether a 70% ethanolic (EtOH) extract of Sargassum horneri had antioxidant and anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated macrophage-like RAW 264.7 cells. [METHODS]: The proximate composition, fatty acids, amino acids, and dietary fiber of S. horneri, various biologically active compounds, and antioxidant activity were analyzed. [RESULTS]: The DPPH and ABTS free radical scavenging activities, as well as the reduction power, of the S. horneri extract used here were significantly increased in a concentration-dependent manner. This indicates that S. horneri contains bioactive compounds, such as phenols and flavonoids, that have excellent antioxidant activity. The cellular viability and metabolic activity results confirmed that the extract had no discernible toxicity at concentrations up to 100 μg/mL. The levels of nitrites and cytokines (PGE(2), TNF-α and IL-6), which mediate pro-inflammatory effect, were significantly inhibited by treatment with either 50 or 100 μg/mL S. horneri extract, whereas that of IL-1β was significantly inhibited by treatment with 100 μg/mL of the extract. Similarly, the expression of iNOS and COX-2 proteins also decreased according to 50 or 100 μg/mL extract concentrations. NF-κB binding to DNA was also significantly inhibited by treatment with 100 μg/mL of extract. [CONCLUSION]: These results suggest that 70% EtOH extracts of S. horneri can relieve inflammation caused by disease or high intensity exercise.
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spelling pubmed-88438432022-02-24 Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages Kim, Min Ju Jo, Hee Geun Ramakrishna, Chilakala Lee, Seung-Jae Lee, Dong-Sung Cheong, Sun Hee Phys Act Nutr Original Article [PURPOSE]: In this study, we investigated whether a 70% ethanolic (EtOH) extract of Sargassum horneri had antioxidant and anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated macrophage-like RAW 264.7 cells. [METHODS]: The proximate composition, fatty acids, amino acids, and dietary fiber of S. horneri, various biologically active compounds, and antioxidant activity were analyzed. [RESULTS]: The DPPH and ABTS free radical scavenging activities, as well as the reduction power, of the S. horneri extract used here were significantly increased in a concentration-dependent manner. This indicates that S. horneri contains bioactive compounds, such as phenols and flavonoids, that have excellent antioxidant activity. The cellular viability and metabolic activity results confirmed that the extract had no discernible toxicity at concentrations up to 100 μg/mL. The levels of nitrites and cytokines (PGE(2), TNF-α and IL-6), which mediate pro-inflammatory effect, were significantly inhibited by treatment with either 50 or 100 μg/mL S. horneri extract, whereas that of IL-1β was significantly inhibited by treatment with 100 μg/mL of the extract. Similarly, the expression of iNOS and COX-2 proteins also decreased according to 50 or 100 μg/mL extract concentrations. NF-κB binding to DNA was also significantly inhibited by treatment with 100 μg/mL of extract. [CONCLUSION]: These results suggest that 70% EtOH extracts of S. horneri can relieve inflammation caused by disease or high intensity exercise. Korean Society for Exercise Nutrition 2021-12 2021-12-31 /pmc/articles/PMC8843843/ /pubmed/35152623 http://dx.doi.org/10.20463/pan.2021.0025 Text en Copyright © 2021 Korean Society for Exercise Nutrition https://creativecommons.org/licenses/by-nc/2.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/ (https://creativecommons.org/licenses/by-nc/2.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Min Ju
Jo, Hee Geun
Ramakrishna, Chilakala
Lee, Seung-Jae
Lee, Dong-Sung
Cheong, Sun Hee
Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages
title Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages
title_full Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages
title_fullStr Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages
title_full_unstemmed Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages
title_short Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages
title_sort anti-inflammatory and antioxidant activities of sargassum horneri extract in raw264.7 macrophages
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843843/
https://www.ncbi.nlm.nih.gov/pubmed/35152623
http://dx.doi.org/10.20463/pan.2021.0025
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