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Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig

Preadipocyte differentiation plays an important role in lipid deposition and affects fattening efficiency in pigs. In the present study, preadipocytes isolated from the subcutaneous adipose tissue of three Landrace piglets were induced into mature adipocytes in vitro. Gene clusters associated with f...

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Autores principales: Zhao, Xitong, Liu, Huatao, Pan, Yongjie, Liu, Yibing, Zhang, Fengxia, Ao, Hong, Zhang, Jibin, Xing, Kai, Wang, Chuduan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843850/
https://www.ncbi.nlm.nih.gov/pubmed/35178067
http://dx.doi.org/10.3389/fgene.2021.753725
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author Zhao, Xitong
Liu, Huatao
Pan, Yongjie
Liu, Yibing
Zhang, Fengxia
Ao, Hong
Zhang, Jibin
Xing, Kai
Wang, Chuduan
author_facet Zhao, Xitong
Liu, Huatao
Pan, Yongjie
Liu, Yibing
Zhang, Fengxia
Ao, Hong
Zhang, Jibin
Xing, Kai
Wang, Chuduan
author_sort Zhao, Xitong
collection PubMed
description Preadipocyte differentiation plays an important role in lipid deposition and affects fattening efficiency in pigs. In the present study, preadipocytes isolated from the subcutaneous adipose tissue of three Landrace piglets were induced into mature adipocytes in vitro. Gene clusters associated with fat deposition were investigated using RNA sequencing data at four time points during preadipocyte differentiation. Twenty-seven co-expression modules were subsequently constructed using weighted gene co-expression network analysis. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses revealed three modules (blue, magenta, and brown) as being the most critical during preadipocyte differentiation. Based on these data and our previous differentially expressed gene analysis, angiopoietin-like 4 (ANGPTL4) was identified as a key regulator of preadipocyte differentiation and lipid metabolism. After inhibition of ANGPTL4, the expression of adipogenesis-related genes was reduced, except for that of lipoprotein lipase (LPL), which was negatively regulated by ANGPTL4 during preadipocyte differentiation. Our findings provide a new perspective to understand the mechanism of fat deposition.
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spelling pubmed-88438502022-02-16 Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig Zhao, Xitong Liu, Huatao Pan, Yongjie Liu, Yibing Zhang, Fengxia Ao, Hong Zhang, Jibin Xing, Kai Wang, Chuduan Front Genet Genetics Preadipocyte differentiation plays an important role in lipid deposition and affects fattening efficiency in pigs. In the present study, preadipocytes isolated from the subcutaneous adipose tissue of three Landrace piglets were induced into mature adipocytes in vitro. Gene clusters associated with fat deposition were investigated using RNA sequencing data at four time points during preadipocyte differentiation. Twenty-seven co-expression modules were subsequently constructed using weighted gene co-expression network analysis. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses revealed three modules (blue, magenta, and brown) as being the most critical during preadipocyte differentiation. Based on these data and our previous differentially expressed gene analysis, angiopoietin-like 4 (ANGPTL4) was identified as a key regulator of preadipocyte differentiation and lipid metabolism. After inhibition of ANGPTL4, the expression of adipogenesis-related genes was reduced, except for that of lipoprotein lipase (LPL), which was negatively regulated by ANGPTL4 during preadipocyte differentiation. Our findings provide a new perspective to understand the mechanism of fat deposition. Frontiers Media S.A. 2022-02-01 /pmc/articles/PMC8843850/ /pubmed/35178067 http://dx.doi.org/10.3389/fgene.2021.753725 Text en Copyright © 2022 Zhao, Liu, Pan, Liu, Zhang, Ao, Zhang, Xing and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhao, Xitong
Liu, Huatao
Pan, Yongjie
Liu, Yibing
Zhang, Fengxia
Ao, Hong
Zhang, Jibin
Xing, Kai
Wang, Chuduan
Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig
title Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig
title_full Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig
title_fullStr Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig
title_full_unstemmed Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig
title_short Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig
title_sort identification of potential candidate genes from co-expression module analysis during preadipocyte differentiation in landrace pig
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843850/
https://www.ncbi.nlm.nih.gov/pubmed/35178067
http://dx.doi.org/10.3389/fgene.2021.753725
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