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Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig
Preadipocyte differentiation plays an important role in lipid deposition and affects fattening efficiency in pigs. In the present study, preadipocytes isolated from the subcutaneous adipose tissue of three Landrace piglets were induced into mature adipocytes in vitro. Gene clusters associated with f...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843850/ https://www.ncbi.nlm.nih.gov/pubmed/35178067 http://dx.doi.org/10.3389/fgene.2021.753725 |
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author | Zhao, Xitong Liu, Huatao Pan, Yongjie Liu, Yibing Zhang, Fengxia Ao, Hong Zhang, Jibin Xing, Kai Wang, Chuduan |
author_facet | Zhao, Xitong Liu, Huatao Pan, Yongjie Liu, Yibing Zhang, Fengxia Ao, Hong Zhang, Jibin Xing, Kai Wang, Chuduan |
author_sort | Zhao, Xitong |
collection | PubMed |
description | Preadipocyte differentiation plays an important role in lipid deposition and affects fattening efficiency in pigs. In the present study, preadipocytes isolated from the subcutaneous adipose tissue of three Landrace piglets were induced into mature adipocytes in vitro. Gene clusters associated with fat deposition were investigated using RNA sequencing data at four time points during preadipocyte differentiation. Twenty-seven co-expression modules were subsequently constructed using weighted gene co-expression network analysis. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses revealed three modules (blue, magenta, and brown) as being the most critical during preadipocyte differentiation. Based on these data and our previous differentially expressed gene analysis, angiopoietin-like 4 (ANGPTL4) was identified as a key regulator of preadipocyte differentiation and lipid metabolism. After inhibition of ANGPTL4, the expression of adipogenesis-related genes was reduced, except for that of lipoprotein lipase (LPL), which was negatively regulated by ANGPTL4 during preadipocyte differentiation. Our findings provide a new perspective to understand the mechanism of fat deposition. |
format | Online Article Text |
id | pubmed-8843850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88438502022-02-16 Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig Zhao, Xitong Liu, Huatao Pan, Yongjie Liu, Yibing Zhang, Fengxia Ao, Hong Zhang, Jibin Xing, Kai Wang, Chuduan Front Genet Genetics Preadipocyte differentiation plays an important role in lipid deposition and affects fattening efficiency in pigs. In the present study, preadipocytes isolated from the subcutaneous adipose tissue of three Landrace piglets were induced into mature adipocytes in vitro. Gene clusters associated with fat deposition were investigated using RNA sequencing data at four time points during preadipocyte differentiation. Twenty-seven co-expression modules were subsequently constructed using weighted gene co-expression network analysis. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses revealed three modules (blue, magenta, and brown) as being the most critical during preadipocyte differentiation. Based on these data and our previous differentially expressed gene analysis, angiopoietin-like 4 (ANGPTL4) was identified as a key regulator of preadipocyte differentiation and lipid metabolism. After inhibition of ANGPTL4, the expression of adipogenesis-related genes was reduced, except for that of lipoprotein lipase (LPL), which was negatively regulated by ANGPTL4 during preadipocyte differentiation. Our findings provide a new perspective to understand the mechanism of fat deposition. Frontiers Media S.A. 2022-02-01 /pmc/articles/PMC8843850/ /pubmed/35178067 http://dx.doi.org/10.3389/fgene.2021.753725 Text en Copyright © 2022 Zhao, Liu, Pan, Liu, Zhang, Ao, Zhang, Xing and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Zhao, Xitong Liu, Huatao Pan, Yongjie Liu, Yibing Zhang, Fengxia Ao, Hong Zhang, Jibin Xing, Kai Wang, Chuduan Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig |
title | Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig |
title_full | Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig |
title_fullStr | Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig |
title_full_unstemmed | Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig |
title_short | Identification of Potential Candidate Genes From Co-Expression Module Analysis During Preadipocyte Differentiation in Landrace Pig |
title_sort | identification of potential candidate genes from co-expression module analysis during preadipocyte differentiation in landrace pig |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843850/ https://www.ncbi.nlm.nih.gov/pubmed/35178067 http://dx.doi.org/10.3389/fgene.2021.753725 |
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