Cargando…

MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green

The “Spring-red-leaf” crabapple cultivar has young red leaves and mature green leaves. However, the mechanism of anthocyanin biosynthesis in crabapple leaves in spring remains unknown. In this study, Illumina RNA sequencing (RNA-Seq) was performed on Malus ‘Radiant’ leaf tissues in different stages...

Descripción completa

Detalles Bibliográficos
Autores principales: Meng, Jia-Xin, Wei, Jun, Chi, Ru-Fei, Qiao, Yu-Hang, Zhou, Jing, Wang, Yi-Lin, Wang, Han, Li, Hou-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843855/
https://www.ncbi.nlm.nih.gov/pubmed/35178062
http://dx.doi.org/10.3389/fpls.2022.822340
_version_ 1784651355597570048
author Meng, Jia-Xin
Wei, Jun
Chi, Ru-Fei
Qiao, Yu-Hang
Zhou, Jing
Wang, Yi-Lin
Wang, Han
Li, Hou-Hua
author_facet Meng, Jia-Xin
Wei, Jun
Chi, Ru-Fei
Qiao, Yu-Hang
Zhou, Jing
Wang, Yi-Lin
Wang, Han
Li, Hou-Hua
author_sort Meng, Jia-Xin
collection PubMed
description The “Spring-red-leaf” crabapple cultivar has young red leaves and mature green leaves. However, the mechanism of anthocyanin biosynthesis in crabapple leaves in spring remains unknown. In this study, Illumina RNA sequencing (RNA-Seq) was performed on Malus ‘Radiant’ leaf tissues in different stages of development. Twenty-two genes in the anthocyanin biosynthesis pathway and 44 MYB transcription factors (TFs) were significantly enriched among differentially expressed genes (DEGs). Three R2R3-MYB TFs in subgroup 22 of the MYB TF family, MrMYB44-like1, MrMYB44-like2, and MrMYB44-like3, were highly expressed in green leaves according to RNA-Seq and quantitative real-time quantitative PCR results. Their expression levels were negatively correlated with anthocyanin content. In transient assays, overexpression of MrMYB44-like1, MrMYB44-like2, or MrMYB44-like3 inhibited anthocyanin accumulation and reduced pigment in leaf disks of M. ‘Radiant’ and fruit peels of M. domestica ‘Fuji.’ When the conserved region of the three MrMYB44-likes was silenced, the anthocyanin biosynthesis pathway was activated and pigments increased in both tissues. Moreover, bimolecular fluorescence complementation assays showed MrMYB44-likes interacted with MrWRKY6 to form protein complexes that regulated anthocyanin biosynthesis.
format Online
Article
Text
id pubmed-8843855
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88438552022-02-16 MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green Meng, Jia-Xin Wei, Jun Chi, Ru-Fei Qiao, Yu-Hang Zhou, Jing Wang, Yi-Lin Wang, Han Li, Hou-Hua Front Plant Sci Plant Science The “Spring-red-leaf” crabapple cultivar has young red leaves and mature green leaves. However, the mechanism of anthocyanin biosynthesis in crabapple leaves in spring remains unknown. In this study, Illumina RNA sequencing (RNA-Seq) was performed on Malus ‘Radiant’ leaf tissues in different stages of development. Twenty-two genes in the anthocyanin biosynthesis pathway and 44 MYB transcription factors (TFs) were significantly enriched among differentially expressed genes (DEGs). Three R2R3-MYB TFs in subgroup 22 of the MYB TF family, MrMYB44-like1, MrMYB44-like2, and MrMYB44-like3, were highly expressed in green leaves according to RNA-Seq and quantitative real-time quantitative PCR results. Their expression levels were negatively correlated with anthocyanin content. In transient assays, overexpression of MrMYB44-like1, MrMYB44-like2, or MrMYB44-like3 inhibited anthocyanin accumulation and reduced pigment in leaf disks of M. ‘Radiant’ and fruit peels of M. domestica ‘Fuji.’ When the conserved region of the three MrMYB44-likes was silenced, the anthocyanin biosynthesis pathway was activated and pigments increased in both tissues. Moreover, bimolecular fluorescence complementation assays showed MrMYB44-likes interacted with MrWRKY6 to form protein complexes that regulated anthocyanin biosynthesis. Frontiers Media S.A. 2022-02-01 /pmc/articles/PMC8843855/ /pubmed/35178062 http://dx.doi.org/10.3389/fpls.2022.822340 Text en Copyright © 2022 Meng, Wei, Chi, Qiao, Zhou, Wang, Wang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Meng, Jia-Xin
Wei, Jun
Chi, Ru-Fei
Qiao, Yu-Hang
Zhou, Jing
Wang, Yi-Lin
Wang, Han
Li, Hou-Hua
MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green
title MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green
title_full MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green
title_fullStr MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green
title_full_unstemmed MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green
title_short MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green
title_sort mrmyb44-like negatively regulates anthocyanin biosynthesis and causes spring leaf color of malus ‘radiant’ to fade from red to green
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843855/
https://www.ncbi.nlm.nih.gov/pubmed/35178062
http://dx.doi.org/10.3389/fpls.2022.822340
work_keys_str_mv AT mengjiaxin mrmyb44likenegativelyregulatesanthocyaninbiosynthesisandcausesspringleafcolorofmalusradianttofadefromredtogreen
AT weijun mrmyb44likenegativelyregulatesanthocyaninbiosynthesisandcausesspringleafcolorofmalusradianttofadefromredtogreen
AT chirufei mrmyb44likenegativelyregulatesanthocyaninbiosynthesisandcausesspringleafcolorofmalusradianttofadefromredtogreen
AT qiaoyuhang mrmyb44likenegativelyregulatesanthocyaninbiosynthesisandcausesspringleafcolorofmalusradianttofadefromredtogreen
AT zhoujing mrmyb44likenegativelyregulatesanthocyaninbiosynthesisandcausesspringleafcolorofmalusradianttofadefromredtogreen
AT wangyilin mrmyb44likenegativelyregulatesanthocyaninbiosynthesisandcausesspringleafcolorofmalusradianttofadefromredtogreen
AT wanghan mrmyb44likenegativelyregulatesanthocyaninbiosynthesisandcausesspringleafcolorofmalusradianttofadefromredtogreen
AT lihouhua mrmyb44likenegativelyregulatesanthocyaninbiosynthesisandcausesspringleafcolorofmalusradianttofadefromredtogreen