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MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green
The “Spring-red-leaf” crabapple cultivar has young red leaves and mature green leaves. However, the mechanism of anthocyanin biosynthesis in crabapple leaves in spring remains unknown. In this study, Illumina RNA sequencing (RNA-Seq) was performed on Malus ‘Radiant’ leaf tissues in different stages...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843855/ https://www.ncbi.nlm.nih.gov/pubmed/35178062 http://dx.doi.org/10.3389/fpls.2022.822340 |
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author | Meng, Jia-Xin Wei, Jun Chi, Ru-Fei Qiao, Yu-Hang Zhou, Jing Wang, Yi-Lin Wang, Han Li, Hou-Hua |
author_facet | Meng, Jia-Xin Wei, Jun Chi, Ru-Fei Qiao, Yu-Hang Zhou, Jing Wang, Yi-Lin Wang, Han Li, Hou-Hua |
author_sort | Meng, Jia-Xin |
collection | PubMed |
description | The “Spring-red-leaf” crabapple cultivar has young red leaves and mature green leaves. However, the mechanism of anthocyanin biosynthesis in crabapple leaves in spring remains unknown. In this study, Illumina RNA sequencing (RNA-Seq) was performed on Malus ‘Radiant’ leaf tissues in different stages of development. Twenty-two genes in the anthocyanin biosynthesis pathway and 44 MYB transcription factors (TFs) were significantly enriched among differentially expressed genes (DEGs). Three R2R3-MYB TFs in subgroup 22 of the MYB TF family, MrMYB44-like1, MrMYB44-like2, and MrMYB44-like3, were highly expressed in green leaves according to RNA-Seq and quantitative real-time quantitative PCR results. Their expression levels were negatively correlated with anthocyanin content. In transient assays, overexpression of MrMYB44-like1, MrMYB44-like2, or MrMYB44-like3 inhibited anthocyanin accumulation and reduced pigment in leaf disks of M. ‘Radiant’ and fruit peels of M. domestica ‘Fuji.’ When the conserved region of the three MrMYB44-likes was silenced, the anthocyanin biosynthesis pathway was activated and pigments increased in both tissues. Moreover, bimolecular fluorescence complementation assays showed MrMYB44-likes interacted with MrWRKY6 to form protein complexes that regulated anthocyanin biosynthesis. |
format | Online Article Text |
id | pubmed-8843855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88438552022-02-16 MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green Meng, Jia-Xin Wei, Jun Chi, Ru-Fei Qiao, Yu-Hang Zhou, Jing Wang, Yi-Lin Wang, Han Li, Hou-Hua Front Plant Sci Plant Science The “Spring-red-leaf” crabapple cultivar has young red leaves and mature green leaves. However, the mechanism of anthocyanin biosynthesis in crabapple leaves in spring remains unknown. In this study, Illumina RNA sequencing (RNA-Seq) was performed on Malus ‘Radiant’ leaf tissues in different stages of development. Twenty-two genes in the anthocyanin biosynthesis pathway and 44 MYB transcription factors (TFs) were significantly enriched among differentially expressed genes (DEGs). Three R2R3-MYB TFs in subgroup 22 of the MYB TF family, MrMYB44-like1, MrMYB44-like2, and MrMYB44-like3, were highly expressed in green leaves according to RNA-Seq and quantitative real-time quantitative PCR results. Their expression levels were negatively correlated with anthocyanin content. In transient assays, overexpression of MrMYB44-like1, MrMYB44-like2, or MrMYB44-like3 inhibited anthocyanin accumulation and reduced pigment in leaf disks of M. ‘Radiant’ and fruit peels of M. domestica ‘Fuji.’ When the conserved region of the three MrMYB44-likes was silenced, the anthocyanin biosynthesis pathway was activated and pigments increased in both tissues. Moreover, bimolecular fluorescence complementation assays showed MrMYB44-likes interacted with MrWRKY6 to form protein complexes that regulated anthocyanin biosynthesis. Frontiers Media S.A. 2022-02-01 /pmc/articles/PMC8843855/ /pubmed/35178062 http://dx.doi.org/10.3389/fpls.2022.822340 Text en Copyright © 2022 Meng, Wei, Chi, Qiao, Zhou, Wang, Wang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Meng, Jia-Xin Wei, Jun Chi, Ru-Fei Qiao, Yu-Hang Zhou, Jing Wang, Yi-Lin Wang, Han Li, Hou-Hua MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green |
title | MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green |
title_full | MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green |
title_fullStr | MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green |
title_full_unstemmed | MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green |
title_short | MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus ‘Radiant’ to Fade From Red to Green |
title_sort | mrmyb44-like negatively regulates anthocyanin biosynthesis and causes spring leaf color of malus ‘radiant’ to fade from red to green |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8843855/ https://www.ncbi.nlm.nih.gov/pubmed/35178062 http://dx.doi.org/10.3389/fpls.2022.822340 |
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