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Anticancer and antimicrobial activity of biosynthesized Red Sea marine algal silver nanoparticles

Biosynthesis of silver nanoparticles (AgNPs) is emerging as a simple and eco-friendly alternative to conventional chemical synthesis methods. The role of AgNPs is expanding as antimicrobial and anticancer agents, sensors, nanoelectronic devices, and imaging contrast agents. In this study, biogenic A...

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Autores principales: Algotiml, Rabaa, Gab-Alla, Ali, Seoudi, Roshdi, Abulreesh, Hussein H., El-Readi, Mahmoud Zaki, Elbanna, Khaled
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844081/
https://www.ncbi.nlm.nih.gov/pubmed/35165346
http://dx.doi.org/10.1038/s41598-022-06412-3
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author Algotiml, Rabaa
Gab-Alla, Ali
Seoudi, Roshdi
Abulreesh, Hussein H.
El-Readi, Mahmoud Zaki
Elbanna, Khaled
author_facet Algotiml, Rabaa
Gab-Alla, Ali
Seoudi, Roshdi
Abulreesh, Hussein H.
El-Readi, Mahmoud Zaki
Elbanna, Khaled
author_sort Algotiml, Rabaa
collection PubMed
description Biosynthesis of silver nanoparticles (AgNPs) is emerging as a simple and eco-friendly alternative to conventional chemical synthesis methods. The role of AgNPs is expanding as antimicrobial and anticancer agents, sensors, nanoelectronic devices, and imaging contrast agents. In this study, biogenic AgNPs were synthesized using extracts of different marine algae species, including Ulva rigida (green alga), Cystoseira myrica (brown alga), and Gracilaria foliifera (red alga), as reducing and capping agents. The Physiochemical properties, cytotoxicity, anticancer and antimicrobial activities of the biosynthesized AgNPs were assessed. Surface plasmonic bands of the biosynthesized AgNPs capped with U. rigida, C. myrica, and G. foliifera extracts were visually observed to determine a colour change, and their peaks were observed at 424 nm, 409 nm, and 415 nm, respectively, by UV–Vis spectroscopy; transmission electron microscopy (TEM) indicated an almost spherical shape of AgNPs with nanoscale sizes of 12 nm, 17 nm, and 24 nm, respectively. Fourier transform-infrared (FTIR) spectroscopy analysis suggested that different molecules attached to AgNPs through OH, C=O, and amide groups. The major constituents of the aqueous algal extracts included, terpenoids, polyphenols, sulfonates, polysaccharides, fatty acids, chlorophylls, amide proteins, flavonoids, carotenoids, aliphatic fluoro compounds, volatile compounds, alkalines, pyruvic acid and agar groups. The cytotoxicity and anticancer activities of the biosynthesized AgNPs were assessed using Artemia salina nauplii, normal skin cell lines (HFb-4), and breast cancer cell lines (MCF-7 cell line). The lethality was found to be directly proportional to the AgNP concentration. The IC(50) values of C. myrica and G. foliifera AgNPs against A. saline nauplii were 5 and 10 μg ml(−1) after 4 h and 16 h, respectively, whereas U. rigida AgNPs did not exhibit cytotoxic effects. Anticancer activity of the biosynthesized AgNPs was dose dependent. The IC(50) values of the biosynthesized AgNPs were 13, 13, and 43 µg ml(−1) for U. rigida, C. myrica, and G. foliifera, respectively. U. rigida AgNPs particularly exhibited potent anticancer activity (92.62%) against a human breast adenocarcinoma cell line (MCF-7) with high selectivity compared the normal cells (IC(50) = 13 µg/ml, SI = 3.2), followed by C. myrica AgNPs (IC(50) = 13 µg/ml, SI = 3.07). Furthermore, the biosynthesized AgNPs exhibited strong antifungal activity against dermatophyte pathogenic moulds and mild antibacterial activity against the food borne pathogen bacteria. The highest antimicrobial activity was recorded for the U. rigida AgNPs, followed by those capped with C. myrica and G. foliifera extracts, respectively. AgNPs capped with the U. rigida extract exhibited the highest antimicrobial activity against Trichophyton mantigrophytes (40 mm), followed by Trichosporon cataneum (30 mm) and E. coli (19 mm), with minimal lethal concentration of 32 and 64 μg ml(−1) respectively. The study finally revealed that extracts of marine algal species, particularly U. rigida extracts, could be effectively used as reducing agents for the green synthesis of AgNPs. These AgNPs are considered efficient alternative antidermatophytes for skin infections and anticancer agents against the MCF-7 cell line.
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spelling pubmed-88440812022-02-16 Anticancer and antimicrobial activity of biosynthesized Red Sea marine algal silver nanoparticles Algotiml, Rabaa Gab-Alla, Ali Seoudi, Roshdi Abulreesh, Hussein H. El-Readi, Mahmoud Zaki Elbanna, Khaled Sci Rep Article Biosynthesis of silver nanoparticles (AgNPs) is emerging as a simple and eco-friendly alternative to conventional chemical synthesis methods. The role of AgNPs is expanding as antimicrobial and anticancer agents, sensors, nanoelectronic devices, and imaging contrast agents. In this study, biogenic AgNPs were synthesized using extracts of different marine algae species, including Ulva rigida (green alga), Cystoseira myrica (brown alga), and Gracilaria foliifera (red alga), as reducing and capping agents. The Physiochemical properties, cytotoxicity, anticancer and antimicrobial activities of the biosynthesized AgNPs were assessed. Surface plasmonic bands of the biosynthesized AgNPs capped with U. rigida, C. myrica, and G. foliifera extracts were visually observed to determine a colour change, and their peaks were observed at 424 nm, 409 nm, and 415 nm, respectively, by UV–Vis spectroscopy; transmission electron microscopy (TEM) indicated an almost spherical shape of AgNPs with nanoscale sizes of 12 nm, 17 nm, and 24 nm, respectively. Fourier transform-infrared (FTIR) spectroscopy analysis suggested that different molecules attached to AgNPs through OH, C=O, and amide groups. The major constituents of the aqueous algal extracts included, terpenoids, polyphenols, sulfonates, polysaccharides, fatty acids, chlorophylls, amide proteins, flavonoids, carotenoids, aliphatic fluoro compounds, volatile compounds, alkalines, pyruvic acid and agar groups. The cytotoxicity and anticancer activities of the biosynthesized AgNPs were assessed using Artemia salina nauplii, normal skin cell lines (HFb-4), and breast cancer cell lines (MCF-7 cell line). The lethality was found to be directly proportional to the AgNP concentration. The IC(50) values of C. myrica and G. foliifera AgNPs against A. saline nauplii were 5 and 10 μg ml(−1) after 4 h and 16 h, respectively, whereas U. rigida AgNPs did not exhibit cytotoxic effects. Anticancer activity of the biosynthesized AgNPs was dose dependent. The IC(50) values of the biosynthesized AgNPs were 13, 13, and 43 µg ml(−1) for U. rigida, C. myrica, and G. foliifera, respectively. U. rigida AgNPs particularly exhibited potent anticancer activity (92.62%) against a human breast adenocarcinoma cell line (MCF-7) with high selectivity compared the normal cells (IC(50) = 13 µg/ml, SI = 3.2), followed by C. myrica AgNPs (IC(50) = 13 µg/ml, SI = 3.07). Furthermore, the biosynthesized AgNPs exhibited strong antifungal activity against dermatophyte pathogenic moulds and mild antibacterial activity against the food borne pathogen bacteria. The highest antimicrobial activity was recorded for the U. rigida AgNPs, followed by those capped with C. myrica and G. foliifera extracts, respectively. AgNPs capped with the U. rigida extract exhibited the highest antimicrobial activity against Trichophyton mantigrophytes (40 mm), followed by Trichosporon cataneum (30 mm) and E. coli (19 mm), with minimal lethal concentration of 32 and 64 μg ml(−1) respectively. The study finally revealed that extracts of marine algal species, particularly U. rigida extracts, could be effectively used as reducing agents for the green synthesis of AgNPs. These AgNPs are considered efficient alternative antidermatophytes for skin infections and anticancer agents against the MCF-7 cell line. Nature Publishing Group UK 2022-02-14 /pmc/articles/PMC8844081/ /pubmed/35165346 http://dx.doi.org/10.1038/s41598-022-06412-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Algotiml, Rabaa
Gab-Alla, Ali
Seoudi, Roshdi
Abulreesh, Hussein H.
El-Readi, Mahmoud Zaki
Elbanna, Khaled
Anticancer and antimicrobial activity of biosynthesized Red Sea marine algal silver nanoparticles
title Anticancer and antimicrobial activity of biosynthesized Red Sea marine algal silver nanoparticles
title_full Anticancer and antimicrobial activity of biosynthesized Red Sea marine algal silver nanoparticles
title_fullStr Anticancer and antimicrobial activity of biosynthesized Red Sea marine algal silver nanoparticles
title_full_unstemmed Anticancer and antimicrobial activity of biosynthesized Red Sea marine algal silver nanoparticles
title_short Anticancer and antimicrobial activity of biosynthesized Red Sea marine algal silver nanoparticles
title_sort anticancer and antimicrobial activity of biosynthesized red sea marine algal silver nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844081/
https://www.ncbi.nlm.nih.gov/pubmed/35165346
http://dx.doi.org/10.1038/s41598-022-06412-3
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