Complete genome sequence of a novel secovirid infecting cassava in the Americas

We report the complete genome sequence of a field isolate of a novel bipartite secovirid infecting cassava in Colombia, provisionally named "cassava torrado-like virus" (CsTLV). The genome sequence was obtained using Oxford Nanopore Technology, and the 5’ ends were confirmed by RACE. The R...

Descripción completa

Detalles Bibliográficos
Autores principales: Leiva, Ana M., Jimenez, Jenyfer, Sandoval, Hector, Perez, Shirley, Cuellar, Wilmer J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2022
Materias:
Annotated Sequence Record
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844172/
https://www.ncbi.nlm.nih.gov/pubmed/34977988
http://dx.doi.org/10.1007/s00705-021-05325-2
Descripción
Sumario:We report the complete genome sequence of a field isolate of a novel bipartite secovirid infecting cassava in Colombia, provisionally named "cassava torrado-like virus" (CsTLV). The genome sequence was obtained using Oxford Nanopore Technology, and the 5’ ends were confirmed by RACE. The RNA1 is 7252 nucleotides (nt) long, encoding a polyprotein of 2336 amino acids (aa) containing the typical “replication block”, conserved torradovirus motifs, and a Maf/Ham1 domain, which is not commonly found in viral genomes. The RNA2 is 4469 nt long and contains two overlapping ORFs encoding proteins of 226 and 1179 aa, showing the characteristic genome arrangement of members of the genus Torradovirus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-05325-2.

Ejemplares similares