Complete genome sequence of a novel secovirid infecting cassava in the Americas

We report the complete genome sequence of a field isolate of a novel bipartite secovirid infecting cassava in Colombia, provisionally named "cassava torrado-like virus" (CsTLV). The genome sequence was obtained using Oxford Nanopore Technology, and the 5’ ends were confirmed by RACE. The R...

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Autores principales: Leiva, Ana M., Jimenez, Jenyfer, Sandoval, Hector, Perez, Shirley, Cuellar, Wilmer J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2022
Materias:
Annotated Sequence Record
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844172/
https://www.ncbi.nlm.nih.gov/pubmed/34977988
http://dx.doi.org/10.1007/s00705-021-05325-2
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author Leiva, Ana M.
Jimenez, Jenyfer
Sandoval, Hector
Perez, Shirley
Cuellar, Wilmer J.
author_facet Leiva, Ana M.
Jimenez, Jenyfer
Sandoval, Hector
Perez, Shirley
Cuellar, Wilmer J.
author_sort Leiva, Ana M.
collection PubMed
description We report the complete genome sequence of a field isolate of a novel bipartite secovirid infecting cassava in Colombia, provisionally named "cassava torrado-like virus" (CsTLV). The genome sequence was obtained using Oxford Nanopore Technology, and the 5’ ends were confirmed by RACE. The RNA1 is 7252 nucleotides (nt) long, encoding a polyprotein of 2336 amino acids (aa) containing the typical “replication block”, conserved torradovirus motifs, and a Maf/Ham1 domain, which is not commonly found in viral genomes. The RNA2 is 4469 nt long and contains two overlapping ORFs encoding proteins of 226 and 1179 aa, showing the characteristic genome arrangement of members of the genus Torradovirus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-05325-2.
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spelling pubmed-88441722022-02-23 Complete genome sequence of a novel secovirid infecting cassava in the Americas Leiva, Ana M. Jimenez, Jenyfer Sandoval, Hector Perez, Shirley Cuellar, Wilmer J. Arch Virol Annotated Sequence Record We report the complete genome sequence of a field isolate of a novel bipartite secovirid infecting cassava in Colombia, provisionally named "cassava torrado-like virus" (CsTLV). The genome sequence was obtained using Oxford Nanopore Technology, and the 5’ ends were confirmed by RACE. The RNA1 is 7252 nucleotides (nt) long, encoding a polyprotein of 2336 amino acids (aa) containing the typical “replication block”, conserved torradovirus motifs, and a Maf/Ham1 domain, which is not commonly found in viral genomes. The RNA2 is 4469 nt long and contains two overlapping ORFs encoding proteins of 226 and 1179 aa, showing the characteristic genome arrangement of members of the genus Torradovirus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-05325-2. Springer Vienna 2022-01-03 2022 /pmc/articles/PMC8844172/ /pubmed/34977988 http://dx.doi.org/10.1007/s00705-021-05325-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Annotated Sequence Record
Leiva, Ana M.
Jimenez, Jenyfer
Sandoval, Hector
Perez, Shirley
Cuellar, Wilmer J.
Complete genome sequence of a novel secovirid infecting cassava in the Americas
title Complete genome sequence of a novel secovirid infecting cassava in the Americas
title_full Complete genome sequence of a novel secovirid infecting cassava in the Americas
title_fullStr Complete genome sequence of a novel secovirid infecting cassava in the Americas
title_full_unstemmed Complete genome sequence of a novel secovirid infecting cassava in the Americas
title_short Complete genome sequence of a novel secovirid infecting cassava in the Americas
title_sort complete genome sequence of a novel secovirid infecting cassava in the americas
topic Annotated Sequence Record
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844172/
https://www.ncbi.nlm.nih.gov/pubmed/34977988
http://dx.doi.org/10.1007/s00705-021-05325-2
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