A protocol for full-rotation soft X-ray tomography of single cells

The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the...

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Autores principales: Chen, Jian-Hua, Vanslembrouck, Bieke, Loconte, Valentina, Ekman, Axel, Cortese, Mirko, Bartenschlager, Ralf, McDermott, Gerry, Larabell, Carolyn A., Le Gros, Mark A., Weinhardt, Venera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844304/
https://www.ncbi.nlm.nih.gov/pubmed/35199039
http://dx.doi.org/10.1016/j.xpro.2022.101176
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author Chen, Jian-Hua
Vanslembrouck, Bieke
Loconte, Valentina
Ekman, Axel
Cortese, Mirko
Bartenschlager, Ralf
McDermott, Gerry
Larabell, Carolyn A.
Le Gros, Mark A.
Weinhardt, Venera
author_facet Chen, Jian-Hua
Vanslembrouck, Bieke
Loconte, Valentina
Ekman, Axel
Cortese, Mirko
Bartenschlager, Ralf
McDermott, Gerry
Larabell, Carolyn A.
Le Gros, Mark A.
Weinhardt, Venera
author_sort Chen, Jian-Hua
collection PubMed
description The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021).
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spelling pubmed-88443042022-02-22 A protocol for full-rotation soft X-ray tomography of single cells Chen, Jian-Hua Vanslembrouck, Bieke Loconte, Valentina Ekman, Axel Cortese, Mirko Bartenschlager, Ralf McDermott, Gerry Larabell, Carolyn A. Le Gros, Mark A. Weinhardt, Venera STAR Protoc Protocol The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021). Elsevier 2022-02-10 /pmc/articles/PMC8844304/ /pubmed/35199039 http://dx.doi.org/10.1016/j.xpro.2022.101176 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chen, Jian-Hua
Vanslembrouck, Bieke
Loconte, Valentina
Ekman, Axel
Cortese, Mirko
Bartenschlager, Ralf
McDermott, Gerry
Larabell, Carolyn A.
Le Gros, Mark A.
Weinhardt, Venera
A protocol for full-rotation soft X-ray tomography of single cells
title A protocol for full-rotation soft X-ray tomography of single cells
title_full A protocol for full-rotation soft X-ray tomography of single cells
title_fullStr A protocol for full-rotation soft X-ray tomography of single cells
title_full_unstemmed A protocol for full-rotation soft X-ray tomography of single cells
title_short A protocol for full-rotation soft X-ray tomography of single cells
title_sort protocol for full-rotation soft x-ray tomography of single cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844304/
https://www.ncbi.nlm.nih.gov/pubmed/35199039
http://dx.doi.org/10.1016/j.xpro.2022.101176
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