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A protocol for full-rotation soft X-ray tomography of single cells

The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the...

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Detalles Bibliográficos
Autores principales:	Chen, Jian-Hua, Vanslembrouck, Bieke, Loconte, Valentina, Ekman, Axel, Cortese, Mirko, Bartenschlager, Ralf, McDermott, Gerry, Larabell, Carolyn A., Le Gros, Mark A., Weinhardt, Venera
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844304/ https://www.ncbi.nlm.nih.gov/pubmed/35199039 http://dx.doi.org/10.1016/j.xpro.2022.101176

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