Cargando…

Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates

The overall success of a pathogenic microbe depends on its ability to efficiently adapt to challenging conditions in the human host. Long-term evolution experiments track and predict adaptive trajectories and have contributed significantly to our understanding of the driving forces of bacterial adap...

Descripción completa

Detalles Bibliográficos
Autores principales: Jeske, Alexander, Arce-Rodriguez, Alejandro, Thöming, Janne G., Tomasch, Jürgen, Häussler, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844440/
https://www.ncbi.nlm.nih.gov/pubmed/35165270
http://dx.doi.org/10.1038/s41522-022-00268-1
_version_ 1784651476841267200
author Jeske, Alexander
Arce-Rodriguez, Alejandro
Thöming, Janne G.
Tomasch, Jürgen
Häussler, Susanne
author_facet Jeske, Alexander
Arce-Rodriguez, Alejandro
Thöming, Janne G.
Tomasch, Jürgen
Häussler, Susanne
author_sort Jeske, Alexander
collection PubMed
description The overall success of a pathogenic microbe depends on its ability to efficiently adapt to challenging conditions in the human host. Long-term evolution experiments track and predict adaptive trajectories and have contributed significantly to our understanding of the driving forces of bacterial adaptation. In this study, we conducted a cross-sectional study instead of long-term longitudinal evolution experiments. We analyzed the transcriptional profiles as well as genomic sequence variations of a large number of clinical Pseudomonas aeruginosa isolates that have been recovered from different infected human sites. Convergent changes in gene expression patterns were found in different groups of clinical isolates. The majority of repeatedly observed expression patterns could be attributed to a defective lasR gene, which encodes the major quorum-sensing regulator LasR. Strikingly, the gene expression pattern of the lasR-defective strains appeared to reflect a transcriptional response that evolves in a direction consistent with growth within a biofilm. In a process of genetic assimilation, lasR-deficient P. aeruginosa isolates appear to constitutively express a biofilm-adapted transcriptional profile and no longer require a respective environmental trigger. Our results demonstrate that profiling the functional consequences of pathoadaptive mutations in clinical isolates reveals long-term evolutionary pathways and may explain the success of lasR mutants in the opportunistic pathogen P. aeruginosa in a clinical context.
format Online
Article
Text
id pubmed-8844440
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-88444402022-03-04 Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates Jeske, Alexander Arce-Rodriguez, Alejandro Thöming, Janne G. Tomasch, Jürgen Häussler, Susanne NPJ Biofilms Microbiomes Article The overall success of a pathogenic microbe depends on its ability to efficiently adapt to challenging conditions in the human host. Long-term evolution experiments track and predict adaptive trajectories and have contributed significantly to our understanding of the driving forces of bacterial adaptation. In this study, we conducted a cross-sectional study instead of long-term longitudinal evolution experiments. We analyzed the transcriptional profiles as well as genomic sequence variations of a large number of clinical Pseudomonas aeruginosa isolates that have been recovered from different infected human sites. Convergent changes in gene expression patterns were found in different groups of clinical isolates. The majority of repeatedly observed expression patterns could be attributed to a defective lasR gene, which encodes the major quorum-sensing regulator LasR. Strikingly, the gene expression pattern of the lasR-defective strains appeared to reflect a transcriptional response that evolves in a direction consistent with growth within a biofilm. In a process of genetic assimilation, lasR-deficient P. aeruginosa isolates appear to constitutively express a biofilm-adapted transcriptional profile and no longer require a respective environmental trigger. Our results demonstrate that profiling the functional consequences of pathoadaptive mutations in clinical isolates reveals long-term evolutionary pathways and may explain the success of lasR mutants in the opportunistic pathogen P. aeruginosa in a clinical context. Nature Publishing Group UK 2022-02-14 /pmc/articles/PMC8844440/ /pubmed/35165270 http://dx.doi.org/10.1038/s41522-022-00268-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jeske, Alexander
Arce-Rodriguez, Alejandro
Thöming, Janne G.
Tomasch, Jürgen
Häussler, Susanne
Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates
title Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates
title_full Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates
title_fullStr Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates
title_full_unstemmed Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates
title_short Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates
title_sort evolution of biofilm-adapted gene expression profiles in lasr-deficient clinical pseudomonas aeruginosa isolates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844440/
https://www.ncbi.nlm.nih.gov/pubmed/35165270
http://dx.doi.org/10.1038/s41522-022-00268-1
work_keys_str_mv AT jeskealexander evolutionofbiofilmadaptedgeneexpressionprofilesinlasrdeficientclinicalpseudomonasaeruginosaisolates
AT arcerodriguezalejandro evolutionofbiofilmadaptedgeneexpressionprofilesinlasrdeficientclinicalpseudomonasaeruginosaisolates
AT thomingjanneg evolutionofbiofilmadaptedgeneexpressionprofilesinlasrdeficientclinicalpseudomonasaeruginosaisolates
AT tomaschjurgen evolutionofbiofilmadaptedgeneexpressionprofilesinlasrdeficientclinicalpseudomonasaeruginosaisolates
AT hausslersusanne evolutionofbiofilmadaptedgeneexpressionprofilesinlasrdeficientclinicalpseudomonasaeruginosaisolates