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A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens
Environmentally sensitive fluorescent probes (including AIEgens) play pivotal roles in numerous biological studies. Many of these functional materials are developed based on the twisted intramolecular charge transfer (TICT) mechanism. However, the TICT tendency of dialkylated amino groups in biocomp...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844555/ https://www.ncbi.nlm.nih.gov/pubmed/34927375 http://dx.doi.org/10.1002/advs.202104609 |
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author | Miao, Rong Li, Jing Wang, Chao Jiang, Xuefeng Gao, Ying Liu, Xiaoling Wang, Dan Li, Xin Liu, Xiaogang Fang, Yu |
author_facet | Miao, Rong Li, Jing Wang, Chao Jiang, Xuefeng Gao, Ying Liu, Xiaoling Wang, Dan Li, Xin Liu, Xiaogang Fang, Yu |
author_sort | Miao, Rong |
collection | PubMed |
description | Environmentally sensitive fluorescent probes (including AIEgens) play pivotal roles in numerous biological studies. Many of these functional materials are developed based on the twisted intramolecular charge transfer (TICT) mechanism. However, the TICT tendency of dialkylated amino groups in biocompatible main‐stream fluorophores (i.e., coumarins and rhodamines) is weak, limiting their sensitivities. Herein, by replacing dialkylated amino donors with an N‐methylpyrrole group to enhance TICT, a simple and general method to engineer highly environmentally sensitive fluorescent probes is reported. This method yields a platter of colorful fluorescent probes that demonstrates outstanding polarity and viscosity sensitivity with large turn‐on ratios (up to 191 times for polarity and 14 times for viscosity), as well as distinct aggregation‐induced emission (AIE) characteristics. The utilities of these probes in both wash‐free bioimaging and protein detections are also successfully demonstrated. It is expected that this molecular design strategy will inspire the creation of many environmentally sensitive probes. |
format | Online Article Text |
id | pubmed-8844555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88445552022-02-24 A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens Miao, Rong Li, Jing Wang, Chao Jiang, Xuefeng Gao, Ying Liu, Xiaoling Wang, Dan Li, Xin Liu, Xiaogang Fang, Yu Adv Sci (Weinh) Research Articles Environmentally sensitive fluorescent probes (including AIEgens) play pivotal roles in numerous biological studies. Many of these functional materials are developed based on the twisted intramolecular charge transfer (TICT) mechanism. However, the TICT tendency of dialkylated amino groups in biocompatible main‐stream fluorophores (i.e., coumarins and rhodamines) is weak, limiting their sensitivities. Herein, by replacing dialkylated amino donors with an N‐methylpyrrole group to enhance TICT, a simple and general method to engineer highly environmentally sensitive fluorescent probes is reported. This method yields a platter of colorful fluorescent probes that demonstrates outstanding polarity and viscosity sensitivity with large turn‐on ratios (up to 191 times for polarity and 14 times for viscosity), as well as distinct aggregation‐induced emission (AIE) characteristics. The utilities of these probes in both wash‐free bioimaging and protein detections are also successfully demonstrated. It is expected that this molecular design strategy will inspire the creation of many environmentally sensitive probes. John Wiley and Sons Inc. 2021-12-19 /pmc/articles/PMC8844555/ /pubmed/34927375 http://dx.doi.org/10.1002/advs.202104609 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Miao, Rong Li, Jing Wang, Chao Jiang, Xuefeng Gao, Ying Liu, Xiaoling Wang, Dan Li, Xin Liu, Xiaogang Fang, Yu A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens |
title | A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens |
title_full | A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens |
title_fullStr | A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens |
title_full_unstemmed | A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens |
title_short | A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens |
title_sort | general method to develop highly environmentally sensitive fluorescent probes and aiegens |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844555/ https://www.ncbi.nlm.nih.gov/pubmed/34927375 http://dx.doi.org/10.1002/advs.202104609 |
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