Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices

Astrocytes are glial cells that exhibit calcium signaling-mediated activity. Here, we present a protocol to monitor and manipulate astrocyte calcium activity from mouse amygdala slices. In the first part of this protocol, we describe the procedure of astrocyte calcium imaging. In the second part, we...

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Autores principales: Baudon, Angel, Clauss-Creusot, Etienne, Darbon, Pascal, Patwell, Ryan, Grinevich, Valery, Charlet, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844720/
https://www.ncbi.nlm.nih.gov/pubmed/35199029
http://dx.doi.org/10.1016/j.xpro.2022.101159
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author Baudon, Angel
Clauss-Creusot, Etienne
Darbon, Pascal
Patwell, Ryan
Grinevich, Valery
Charlet, Alexandre
author_facet Baudon, Angel
Clauss-Creusot, Etienne
Darbon, Pascal
Patwell, Ryan
Grinevich, Valery
Charlet, Alexandre
author_sort Baudon, Angel
collection PubMed
description Astrocytes are glial cells that exhibit calcium signaling-mediated activity. Here, we present a protocol to monitor and manipulate astrocyte calcium activity from mouse amygdala slices. In the first part of this protocol, we describe the procedure of astrocyte calcium imaging. In the second part, we detail how to disrupt astrocyte calcium activity by patch-clamp-mediated loading of BAPTA. These two approaches are presented separately but they can also be used simultaneously to monitor the effects of disruption on an astrocyte network. For complete details on the use and execution of this protocol, please refer to Wahis et al. (2021).
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spelling pubmed-88447202022-02-22 Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices Baudon, Angel Clauss-Creusot, Etienne Darbon, Pascal Patwell, Ryan Grinevich, Valery Charlet, Alexandre STAR Protoc Protocol Astrocytes are glial cells that exhibit calcium signaling-mediated activity. Here, we present a protocol to monitor and manipulate astrocyte calcium activity from mouse amygdala slices. In the first part of this protocol, we describe the procedure of astrocyte calcium imaging. In the second part, we detail how to disrupt astrocyte calcium activity by patch-clamp-mediated loading of BAPTA. These two approaches are presented separately but they can also be used simultaneously to monitor the effects of disruption on an astrocyte network. For complete details on the use and execution of this protocol, please refer to Wahis et al. (2021). Elsevier 2022-02-08 /pmc/articles/PMC8844720/ /pubmed/35199029 http://dx.doi.org/10.1016/j.xpro.2022.101159 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Baudon, Angel
Clauss-Creusot, Etienne
Darbon, Pascal
Patwell, Ryan
Grinevich, Valery
Charlet, Alexandre
Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices
title Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices
title_full Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices
title_fullStr Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices
title_full_unstemmed Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices
title_short Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices
title_sort calcium imaging and bapta loading of amygdala astrocytes in mouse brain slices
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844720/
https://www.ncbi.nlm.nih.gov/pubmed/35199029
http://dx.doi.org/10.1016/j.xpro.2022.101159
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