Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging

Epithelial cells lining the oviduct/fallopian tube are essential in reproduction and have been identified as the cell-of-origin in high-grade serous ovarian carcinoma (HGSOC). This protocol describes the generation of organoids from mouse oviduct epithelial cells, providing a powerful in vitro tool...

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Autores principales: Ford, Matthew J., Harwalkar, Keerthana, Yamanaka, Yojiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844721/
https://www.ncbi.nlm.nih.gov/pubmed/35199031
http://dx.doi.org/10.1016/j.xpro.2022.101164
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author Ford, Matthew J.
Harwalkar, Keerthana
Yamanaka, Yojiro
author_facet Ford, Matthew J.
Harwalkar, Keerthana
Yamanaka, Yojiro
author_sort Ford, Matthew J.
collection PubMed
description Epithelial cells lining the oviduct/fallopian tube are essential in reproduction and have been identified as the cell-of-origin in high-grade serous ovarian carcinoma (HGSOC). This protocol describes the generation of organoids from mouse oviduct epithelial cells, providing a powerful in vitro tool to study epithelial homeostasis and malignant transformation. We also outline a protocol for whole-mount immunofluorescence and 3D confocal imaging. In addition, we describe approaches of viral transduction to investigate gene function in organoid development and epithelial cell behavior. For complete details on the use and execution of this profile, please refer to Ford et al. (2021).
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spelling pubmed-88447212022-02-22 Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging Ford, Matthew J. Harwalkar, Keerthana Yamanaka, Yojiro STAR Protoc Protocol Epithelial cells lining the oviduct/fallopian tube are essential in reproduction and have been identified as the cell-of-origin in high-grade serous ovarian carcinoma (HGSOC). This protocol describes the generation of organoids from mouse oviduct epithelial cells, providing a powerful in vitro tool to study epithelial homeostasis and malignant transformation. We also outline a protocol for whole-mount immunofluorescence and 3D confocal imaging. In addition, we describe approaches of viral transduction to investigate gene function in organoid development and epithelial cell behavior. For complete details on the use and execution of this profile, please refer to Ford et al. (2021). Elsevier 2022-02-09 /pmc/articles/PMC8844721/ /pubmed/35199031 http://dx.doi.org/10.1016/j.xpro.2022.101164 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ford, Matthew J.
Harwalkar, Keerthana
Yamanaka, Yojiro
Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging
title Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging
title_full Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging
title_fullStr Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging
title_full_unstemmed Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging
title_short Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging
title_sort protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3d imaging
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844721/
https://www.ncbi.nlm.nih.gov/pubmed/35199031
http://dx.doi.org/10.1016/j.xpro.2022.101164
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