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TRACE-seq: A transgenic system for unbiased and non-invasive transcriptome profiling of living cells

Dynamic profiling of changes in gene expression in response to stressors in specific microenvironments without requiring cellular destruction remains challenging. Current methodologies that seek to interrogate gene expression at a molecular level require sampling of cellular transcriptome and theref...

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Detalles Bibliográficos
Autores principales: Cherbonneau, François, Li, Guoping, Gokulnath, Priyanka, Sahu, Parul, Prunevieille, Aurore, Kitchen, Robert, Benichou, Gilles, Larghero, Jérôme, Domian, Ibrahim, Das, Saumya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844816/
https://www.ncbi.nlm.nih.gov/pubmed/35198871
http://dx.doi.org/10.1016/j.isci.2022.103806
Descripción
Sumario:Dynamic profiling of changes in gene expression in response to stressors in specific microenvironments without requiring cellular destruction remains challenging. Current methodologies that seek to interrogate gene expression at a molecular level require sampling of cellular transcriptome and therefore lysis of the cell, preventing serial analysis of cellular transcriptome. To address this area of unmet need, we have recently developed a technology allowing transcriptomic analysis over time without cellular destruction. Our method, TRACE-seq (TRanscriptomic Analysis Captured in Extracellular vesicles using sequencing), is characterized by a cell-type specific transgene expression. It provides data on the transcriptome inside extracellular vesicles that provides an accurate representation of stress-responsive cellular transcriptomic changes. Thus, the transcriptome of cells expressing TRACE can be followed over time without destroying the source cell, which is a powerful tool for many fields of fundamental and translational biology research.